Reverse transcription primers and methods of design
    1.
    发明授权
    Reverse transcription primers and methods of design 有权
    逆转录引物和设计方法

    公开(公告)号:US08268987B2

    公开(公告)日:2012-09-18

    申请号:US12950179

    申请日:2010-11-19

    IPC分类号: C12N15/11 C07H21/04

    摘要: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.

    摘要翻译: 本发明提供了用于设计寡核苷酸的新颖算法,所述寡核苷酸与大部分其他转录物杂交,基本上不与少量不想要的转录物杂交。 这样的寡核苷酸特别可用作逆转录的引物。 本发明还提供了含有寡核苷酸的组合物,其与大部分其他转录物杂交而基本上不与少量不想要的转录物杂交。

    REVERSE TRANSCRIPTION PRIMERS AND METHODS OF DESIGN
    2.
    发明申请
    REVERSE TRANSCRIPTION PRIMERS AND METHODS OF DESIGN 有权
    反向转录原型和设计方法

    公开(公告)号:US20110257039A1

    公开(公告)日:2011-10-20

    申请号:US12950179

    申请日:2010-11-19

    IPC分类号: C40B40/06 C40B50/00

    摘要: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.

    摘要翻译: 本发明提供了用于设计寡核苷酸的新颖算法,所述寡核苷酸与大部分其他转录物杂交,基本上不与少量不想要的转录物杂交。 这样的寡核苷酸特别可用作逆转录的引物。 本发明还提供了含有寡核苷酸的组合物,其与大部分其他转录物杂交而基本上不与少量不想要的转录物杂交。

    Reverse transcription primers and methods of design
    3.
    发明授权
    Reverse transcription primers and methods of design 有权
    逆转录引物和设计方法

    公开(公告)号:US08809513B2

    公开(公告)日:2014-08-19

    申请号:US13592278

    申请日:2012-08-22

    摘要: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.

    摘要翻译: 本发明提供了用于设计寡核苷酸的新颖算法,所述寡核苷酸与大部分其他转录物杂交,基本上不与少量不想要的转录物杂交。 这样的寡核苷酸特别可用作逆转录的引物。 本发明还提供了含有寡核苷酸的组合物,其与大部分其他转录物杂交而基本上不与少量不想要的转录物杂交。

    Recombinant reverse transcriptases
    5.
    发明申请
    Recombinant reverse transcriptases 有权
    重组逆转录酶

    公开(公告)号:US20050232934A1

    公开(公告)日:2005-10-20

    申请号:US10827498

    申请日:2004-04-19

    CPC分类号: C12N9/1276 C12Y207/07049

    摘要: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

    摘要翻译: 本发明涉及编码具有DNA聚合酶活性和显着降低的RNA酶H活性的多反应逆转录酶的基因,含有本发明转化的基因和宿主细胞的载体。 本发明还包括使用本发明的逆转录酶产生过度逆转录酶,从mRNA产生cDNA的方法,使用过度反转录酶制备的试剂盒和测定模板。