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公开(公告)号：US08771985B2
公开(公告)日：2014-07-08
申请号：US13066802
申请日：2011-04-25
CPC分类号： C12N9/22 , A01K67/0275 , A01K2207/05 , A01K2217/07 , A01K2227/105 , A01K2267/03 , A61K38/00 , C07K2319/81 , C12N15/102 , C12N15/907
摘要： Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白的罗莎基因座的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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公开(公告)号：US20120017290A1
公开(公告)日：2012-01-19
申请号：US13066802
申请日：2011-04-25
IPC分类号： A01K67/033 , C12N15/62 , C12N15/01 , A61K38/46 , A61K31/7088 , C12N9/96 , C12N5/10
CPC分类号： C12N9/22 , A01K67/0275 , A01K2207/05 , A01K2217/07 , A01K2227/105 , A01K2267/03 , A61K38/00 , C07K2319/81 , C12N15/102 , C12N15/907
摘要： Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白的罗莎基因座的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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3.发明授权Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases 有权使用工程化锌指蛋白核酸酶靶向破坏T细胞受体基因公开(公告)号：US08956828B2
公开(公告)日：2015-02-17
申请号：US12927292
申请日：2010-11-10
申请人： Maria Chiara Bonini , Pietro Genovese , Philip D. Gregory , Michael C. Holmes , Luigi Naldini , David Paschon , Elena Provasi , Lei Zhang
发明人： Maria Chiara Bonini , Pietro Genovese , Philip D. Gregory , Michael C. Holmes , Luigi Naldini , David Paschon , Elena Provasi , Lei Zhang
CPC分类号： A61K35/28 , A61K35/00 , A61K35/17 , A61K38/00 , C07K14/7051 , C07K2319/81 , C07K2319/95 , C12N9/22 , C12N15/01
摘要： Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
摘要翻译： 本文公开了在能够保持细胞活力的条件下使用锌指核酸酶（ZFN）灭活TCR基因的方法和组合物，所述锌指核酸酶包含锌指蛋白和切割结构域或切割半结构域。 还提供了编码ZFN的多核苷酸，包含编码ZFN的多核苷酸的载体和包含编码ZFN和/或包含ZFN的细胞的多核苷酸的细胞。 本文公开的也是在T淋巴细胞（包括具有中枢记忆表型的淋巴细胞）中不存在内源性TCR表达时表达功能性外源性TCR的方法和组合物。 还提供了编码外源性TCR的多核苷酸，包含编码外源性TCR的多核苷酸的载体和包含编码外源性TCR的多核苷酸的细胞和/或包含外源TCR的细胞。
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公开(公告)号：US20120060230A1
公开(公告)日：2012-03-08
申请号：US13188417
申请日：2011-07-21
申请人： Trevor Collingwood , Laurence J.N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
发明人： Trevor Collingwood , Laurence J.N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
IPC分类号： A61K48/00 , C12N15/11 , A61P5/38 , A61P19/00 , A61P37/00 , A61P7/02 , A61P3/00 , A61P31/14 , A61P5/14 , A61P29/00 , A61P17/06 , A61P19/02 , A61P17/00 , A61P19/04 , C12N9/96 , C12N15/63 , C12N1/19 , C12N5/10 , C12N5/071 , C12N5/0775 , C12N5/0735 , C12N5/0797 , A01K67/027 , C07K7/06
CPC分类号： C07K14/70539 , A61K35/17 , C07K2319/81 , C12N15/907 , C12N2800/80
摘要： Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.
摘要翻译： 本文公开了用于调节HLA基因座表达或选择性删除或操纵HLA基因座或HLA调节子的方法和组合物。
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5.发明申请Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases 有权使用工程化锌指蛋白核酸酶靶向破坏T细胞受体基因公开(公告)号：US20110158957A1
公开(公告)日：2011-06-30
申请号：US12927292
申请日：2010-11-10
申请人： Maria Chiara Bonini , Pietro Genovese , Philip D. Gregory , Michael C. Holmes , Luigi Naldini , David Paschon , Elena Provasi , Lei Zhang
发明人： Maria Chiara Bonini , Pietro Genovese , Philip D. Gregory , Michael C. Holmes , Luigi Naldini , David Paschon , Elena Provasi , Lei Zhang
IPC分类号： A61K35/12 , C12N9/16 , C07H21/04 , C12N15/63 , C12N5/07 , C12N5/00 , A61P35/00 , A61P37/06 , A61P31/00
CPC分类号： A61K35/28 , A61K35/00 , A61K35/17 , A61K38/00 , C07K14/7051 , C07K2319/81 , C07K2319/95 , C12N9/22 , C12N15/01
摘要： Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
摘要翻译： 本文公开了在能够保持细胞活力的条件下使用锌指核酸酶（ZFN）灭活TCR基因的方法和组合物，所述锌指核酸酶包含锌指蛋白和切割结构域或切割半结构域。 还提供了编码ZFN的多核苷酸，包含编码ZFN的多核苷酸的载体和包含编码ZFN和/或包含ZFN的细胞的多核苷酸的细胞。 本文公开的也是在T淋巴细胞（包括具有中枢记忆表型的淋巴细胞）中不存在内源性TCR表达时表达功能性外源性TCR的方法和组合物。 还提供了编码外源性TCR的多核苷酸，包含编码外源性TCR的多核苷酸的载体和包含编码外源性TCR的多核苷酸的细胞和/或包含外源TCR的细胞。
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公开(公告)号：US20110265198A1
公开(公告)日：2011-10-27
申请号：US13066801
申请日：2011-04-25
IPC分类号： C12N15/01 , A61K38/46 , C12N15/85 , A61P43/00 , C12N9/16 , C12N5/073 , C07H21/04 , A61K31/7088
CPC分类号： C12N9/22 , A01K67/0275 , A01K2207/05 , A01K2217/07 , A01K2227/105 , A01K2267/03 , A61K38/00 , C07K2319/81 , C12N15/102 , C12N15/907
摘要： Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a DNA binding domain and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含DNA结合结构域和切割结构域或裂解半结构域的融合蛋白的罗莎基因座的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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公开(公告)号：US20090263900A1
公开(公告)日：2009-10-22
申请号：US12386059
申请日：2009-04-13
CPC分类号： C12N15/907 , C12N15/63 , C12N15/8213
摘要： Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
摘要翻译： 本文公开了包含侧翼于一个或多个感兴趣序列的50-750个碱基对（例如，50-100个碱基对）的同源臂的线性供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列靶向整合到细胞基因组中的特定感兴趣区域的方法中。
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公开(公告)号：US08945868B2
公开(公告)日：2015-02-03
申请号：US13188417
申请日：2011-07-21
申请人： Trevor Collingwood , Laurence J. N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
发明人： Trevor Collingwood , Laurence J. N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
CPC分类号： C07K14/70539 , A61K35/17 , C07K2319/81 , C12N15/907 , C12N2800/80
摘要： Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.
摘要翻译： 本文公开了用于调节HLA基因座表达或选择性删除或操纵HLA基因座或HLA调节剂的方法和组合物。
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公开(公告)号：US08563314B2
公开(公告)日：2013-10-22
申请号：US12927557
申请日：2010-11-17
申请人： Philip D. Gregory , Michael C. Holmes , Matthew C. Mendel , Xiangdong Meng , David Paschon , Andreas Reik , Fyodor Urnov
发明人： Philip D. Gregory , Michael C. Holmes , Matthew C. Mendel , Xiangdong Meng , David Paschon , Andreas Reik , Fyodor Urnov
CPC分类号： A61K38/1709 , A61K35/17 , A61P31/12 , A61P35/00 , C07K2319/71 , C12N9/22
摘要： Disclosed herein are methods and compositions for modulating expression of a PD1 gene.
摘要翻译： 本文公开了调节PD1基因表达的方法和组合物。
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公开(公告)号：US20110027235A1
公开(公告)日：2011-02-03
申请号：US12798749
申请日：2010-04-08
申请人： Philip D. Gregory , Dirk Hockemeyer , Michael C. Holmes , Rudolf Jaenisch , Frank Soldner , Fyodor Urnov , Shuyuan Yao
发明人： Philip D. Gregory , Dirk Hockemeyer , Michael C. Holmes , Rudolf Jaenisch , Frank Soldner , Fyodor Urnov , Shuyuan Yao
CPC分类号： C12N15/86 , C12N9/22 , C12N2710/10343 , C12N2740/15043 , C12N2800/30 , C12N2830/008
摘要： Disclosed herein are methods and compositions for targeted integration of sequences of interest such as lineage-specific or cell fate reporter constructs or protein encoding sequences into stem cells.
摘要翻译： 本文公开了用于靶向整合感兴趣序列的方法和组合物，例如谱系特异性或细胞命运报道构建体或蛋白质编码序列到干细胞中。
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