摘要:
A method of producing immunoglobulin preparations for intravenous injection which starts with an immunoblobulin-containing fraction and comprises the treatment steps of:(a) treating said fraction with 4-10 weight/volume percent of polyethylene glycol having a molecular weight of 1,000-10,000 under conditions of pH 4-6, ion strength 0.0001-0.1M and temperature 0.degree.-4.degree. C. and recovering the supernatant,(b) treating the supernatant obtained in step (a) with 10-15 weight/volume percent of polyethylene glycol having a molecular weight of 1,000-10,000 under conditions of pH 6-9, ion strength 0.0001-0.1M and temperature 0.degree.-4.degree. C. and recovering the resulting precipitate, and(c) heat-treating, in any desired step, said immunoglobulin in the presence of a stabilizer under conditions sufficient to inactivate contaminant viruses.The preparations obtained according to the invention retain immunoglobulins without substantial inactivation thereof, and are substantially free of such contaminants as anti-human blood group substance antibodies. With contaminant viruses inactivated as a result of the heat treatment, said preparations have good solubility and are sufficiently low in anticomplement activity.
摘要:
An intravenously injectable solution of chemically unmodified .gamma.-globulin having a complete molecular structure is disclosed, said solution containing sorbitol as a stabilizer and having a low electrical conductivity and a pH of about 5.5.+-.0.2. The injectable solution does not cause an increase of .gamma.-globulin polymer, a rise of anticomplement titer, or impairment of the activities of the .gamma.-globulin, either during preservation or upon administration to a living body.
摘要:
A process for heat treating an aqueous solution containing chemically unmodified .gamma.-globulin, wherein said heat treating is carried out in the presence of sorbitol is disclosed. By the heat treatment, impurity viruses can be inactivated without causing impairment of activities of chemically unmodified .gamma.-globulin, increasing polymer contents or increasing an anticomplement titer.
摘要:
Virus-contaminated immunoglobulin can be virus-inactivated by heating it in a substantially dry state at a temperature of 30.degree. to 100.degree. C. for a period of time sufficient for inactivating virus with maintaining original activity of the immunoglobulin. The addition of glycine, sodium chloride, sodium acetate, polyethylene glycol, albumin or mannitol enhances the effect and gives good solubility and good state to the solution of the virus-inactivated immunoglobulin.
摘要:
A method of producing a virus-inactivated protein-containing composition from a protein-containing composition which may be contaminated with virus. The method according to the present invention permits production of pharmaceutically safer virus-inactivated protein preparations without spoiling the protein activity.
摘要:
A method of treating hypoproteinemia is disclosed in which an enteric film coated IgA-rich gamma globulin is administered orally to a person suffering therefrom until total serum content and plasma content are restored. The film coating is soluble in neutral or alkaline media by insoluble in acid media.
摘要:
Highly purified urokinase is obtained in a high yield by adsorbing urokinase from an aqueous crude urokuinase solution including human urine at a pH of about 4.0 to about 7.0 on a water-insoluble polysaccharide containing agarose and agaropectin, for example, agar and Sepharose, and by eluting urokinase with an alkaline solution (pH of about 8 to 11) or concentrated salt solution.
摘要:
A method for stabilizing plasminogen which comprises adding a physiologically acceptable inorganic salt to a plaminogen-containing aqueous solution to give a final concentration of the salt of 0.002 to 0.4 M.
摘要:
A process for recovering interferon which comprises contacting a solution containing an interferon produced by the induced cells of human origin with a water-insolubilized sulfated polysaccharide to allow the interferon to be adsorbed on the water-insolubilized sulfated polysaccharide and then selectively eluting the interferon with an aqueous solution of an inorganic salt.
摘要:
An antithrombin preparation, secure and stable for a long period of time, can be obtained by freeze-drying an aqueous solution containing antithrombin-III as main component in the presence of an effective quantity of at least one member selected from the group consisting of proteins, sugars, amino acids, inorganic salts and salts of organic acids as stabilizer.