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1.发明授权Bacteria containing aspartate-semialdehyde dehydrogenase, phosphoenolpyruvate carboxylase, and transhydrogenase to produce L-lysine in escherichia, and methods of using same 有权含有天冬氨酸 - 半醛脱氢酶，磷酸烯醇丙酮酸羧化酶和转氢酶在埃希氏菌中产生L-赖氨酸的细菌及其使用方法公开(公告)号：US07723081B1
公开(公告)日：2010-05-25
申请号：US10149450
申请日：2000-01-21
申请人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
发明人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
IPC分类号： C12P13/08
摘要： An Escherichia bacterium (1) which harbors dihydrodipicolinate synthase of which feedback inhibition by L-lysine is desensitized and aspartokinase of which feedback inhibition by L-lysine is desensitized, (2) in which intracellular activity of dihydrodipicolinate reductase is enhanced, and (3) in which a diaminopimelate dehydrogenase gene is introduced or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase are enhanced, wherein intracellular activity of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase is enhanced, is cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.
摘要翻译： 具有L-赖氨酸的反馈抑制脱敏的二氢吡啶并吡啶合酶的大肠杆菌细菌（1），L-赖氨酸的反馈抑制的天冬氨酸脱敏，（2）二氢吡啶二羧酸还原酶的细胞内活性增强，（3） 其中引入二氨基庚二酸脱氢酶基因或增加四氢二吡啶甲酰基琥珀酰基酶和琥珀酰二氨基庚二酸脱酰基酶的细胞内活性，其中天冬氨酸半醛脱氢酶或磷酸烯醇丙酮酸羧化酶的细胞内活性增强，在合适的培养基中培养以在培养物中产生和积累L-赖氨酸 ，并从培养物中收集L-赖氨酸。
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公开(公告)号：US08062869B2
公开(公告)日：2011-11-22
申请号：US12721813
申请日：2010-03-11
申请人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
发明人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
IPC分类号： C12P13/08
摘要： An Escherichia bacterium having dihydrodipicolinate synthase and aspartokinase, both of which are desensitized to feedback inhibition by L-lysine. The intracellular activity of dihydrodipicolinate reductase in this bacterium can also be enhanced. Furthermore, a diaminopimelate dehydrogenase gene can be introduced into this bacterium, or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase can be enhanced. Finally, the intracellular activities of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase can be enhanced in this bacterium. The bacterium can be cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.
摘要翻译： 具有二氢吡啶二羧酸合成酶和天冬氨酸激酶的埃希氏菌属细菌，它们都被L-赖氨酸反馈抑制脱敏。 该细菌中二氢吡啶二糖还原酶的细胞内活性也可以提高。 此外，可以向该细菌中引入二氨基庚二酸脱氢酶基因，或者可以提高四氢吡啶二酸酐琥珀酰基酶和琥珀酰二氨基庚二酸酯脱酰酶的细胞内活性。 最后，这种细菌可以增强天冬氨酸半醛脱氢酶或磷酸烯醇丙酮酸羧化酶的细胞内活性。 可以在合适的培养基中培养细菌以在培养物中产生和积累L-赖氨酸，并从培养物中收集L-赖氨酸。
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公开(公告)号：US20100173368A1
公开(公告)日：2010-07-08
申请号：US12721813
申请日：2010-03-11
申请人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
发明人： Kazuo Nakanishi , Yoshimi Kikuchi , Junichiro Kojima , Tomoko Suzuki , Yasushi Nishimura , Hiroyuki Kojima
IPC分类号： C12P13/08
摘要： An Escherichia bacterium having dihydrodipicolinate synthase and aspartokinase, both of which are desensitized to feedback inhibition by L-lysine. The intracellular activity of dihydrodipicolinate reductase in this bacterium can also be enhanced. Furthermore, a diaminopimelate dehydrogenase gene can be introduced into this bacterium, or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase can be enhanced. Finally, the intracellular activities of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase can be enhanced in this bacterium. The bacterium can be cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.
摘要翻译： 具有二氢吡啶二羧酸合成酶和天冬氨酸激酶的埃希氏菌属细菌，它们都被L-赖氨酸反馈抑制脱敏。 该细菌中二氢吡啶二糖还原酶的细胞内活性也可以提高。 此外，可以向该细菌中引入二氨基庚二酸脱氢酶基因，或者可以提高四氢吡啶二酸酐琥珀酰基酶和琥珀酰二氨基庚二酸酯脱酰酶的细胞内活性。 最后，这种细菌可以增强天冬氨酸半醛脱氢酶或磷酸烯醇丙酮酸羧化酶的细胞内活性。 可以在合适的培养基中培养细菌以在培养物中产生和积累L-赖氨酸，并从培养物中收集L-赖氨酸。
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公开(公告)号：US5932453A
公开(公告)日：1999-08-03
申请号：US950737
申请日：1997-10-15
IPC分类号： C12N15/09 , C07C229/26 , C07H21/04 , C12N1/21 , C12N9/12 , C12N15/00 , C12N15/54 , C12P13/08 , C12R1/19 , C12N15/63
CPC分类号： C12N9/1217 , C12P13/08
摘要： A DNA encoding aspartokinase III derived from bacteria of the genus Escherichia and having mutation by which feedback inhibition with L-lysine is released is introduced into cells to form transformant bacteria of the genus Escherichia. These bacteria are incubated in an appropriate culture medium. An L-amino acid is produced and accumulated in the culture, and collected from this culture.
摘要翻译： 将来自埃希氏杆菌属的细菌的天冬氨酸激酶III的DNA和释放了L-赖氨酸的反馈抑制的突变导入细胞，形成埃希氏杆菌属的转化细菌。 将这些细菌在合适的培养基中培养。 在培养物中产生并积累L-氨基酸，并从该培养物中收集。
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公开(公告)号：US5827698A
公开(公告)日：1998-10-27
申请号：US849212
申请日：1997-06-09
申请人： Yoshimi Kikuchi , Tomoko Suzuki , Hiroyuki Kojima
发明人： Yoshimi Kikuchi , Tomoko Suzuki , Hiroyuki Kojima
摘要： L-lysine is produced efficiently by cultivating, in a liquid medium, a microorganism belonging to the genus Escherichia with decreased or disappeared lysine decarboxylase activity relevant to decomposition of L-lysine, for example, a bacterium belonging to the genus Escherichia with restrained expression of a novel gene coding for lysine decarboxylase and/or a known gene cadA to allow L-lysine to be produced and accumulated in a culture liquid, and collecting it.
摘要翻译： PCT No.PCT / JP95 / 02481 Sec。 371日期：1997年6月9日 102（e）日期1997年6月9日PCT提交1995年12月5日PCT公布。 出版物WO96 / 17930 PCT 日期：1996年6月13日L-赖氨酸通过在液体培养基中培养属于埃希氏杆菌属的微生物，与赖氨酸分解相关的赖氨酸脱羧酶活性降低或消失，例如属于该属的细菌 大肠杆菌具有限制性表达编码赖氨酸脱羧酶的新基因和/或已知基因cadA以允许L-赖氨酸在培养液中产生和积累并收集。
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公开(公告)号：US07972829B2
公开(公告)日：2011-07-05
申请号：US12714151
申请日：2010-02-26
IPC分类号： C12N9/10 , C12N1/20 , C12N15/00 , C12P21/04 , A61K38/00 , C07H21/04 , C07H21/02 , C07K1/00 , C12Q1/68 , C12Q1/00
CPC分类号： C12N9/1044 , C07K2319/02 , C07K2319/036 , C12N9/52 , C12N15/625 , C12Y203/02013
摘要： The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.
摘要翻译： 本发明涉及通过棒状细菌分泌外源蛋白质，特别是转谷氨酰胺酶的方法。 根据本发明，提供了通过制备棒状细菌以产生工业上有用的外源蛋白质，特别是转谷氨酰胺酶并有效地将细胞外释放产物（即，通过转运谷氨酰胺酶）分泌外源蛋白质，特别是转谷氨酰胺酶的方法， 分泌生产）。 通过使用表达构建体产生预期的外源蛋白质，特别是转谷氨酰胺酶，其中含有前结构部分，特别是原转谷氨酰胺酶基因序列的预期外源蛋白的基因序列连接到序列的下游 编码来自棒状细菌的信号肽区域，将该表达遗传构建体引入棒状杆菌型细菌，培养由此转化的棒状杆菌型细菌，用蛋白酶等处理细胞外释放的蛋白质以切割和消除前体部分。
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公开(公告)号：US20110137007A1
公开(公告)日：2011-06-09
申请号：US12983500
申请日：2011-01-03
CPC分类号： C12P13/008 , C08G73/22 , C12N9/16 , C12N9/88
摘要： Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.
摘要翻译： 提供了通过培养具有编码不经反馈抑制的突变型天冬氨酸激酶的基因的棒状细菌，并且用含有编码的DNA的重组载体转化的有效产生3-氨基-4-羟基苯甲酸型化合物的方法 具有从磷酸二羟丙酮和天冬氨酸半醛形成3-氨基-4-羟基苯甲酸的活性的蛋白质。
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公开(公告)号：US20100297729A1
公开(公告)日：2010-11-25
申请号：US12714151
申请日：2010-02-26
CPC分类号： C12N9/1044 , C07K2319/02 , C07K2319/036 , C12N9/52 , C12N15/625 , C12Y203/02013
摘要： The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.
摘要翻译： 本发明涉及通过棒状细菌分泌外源蛋白质，特别是转谷氨酰胺酶的方法。 根据本发明，提供了通过制备棒状细菌以产生工业上有用的外源蛋白质，特别是转谷氨酰胺酶并有效地释放产物的方法（即，通过使细胞外有效释放外源蛋白质，特别是转谷氨酰胺酶） 分泌生产）。 通过使用表达构建体产生预期的外源蛋白质，特别是转谷氨酰胺酶，其中含有前结构部分，特别是原转谷氨酰胺酶基因序列的预期外源蛋白的基因序列连接到序列的下游 编码来自棒状细菌的信号肽区域，将该表达遗传构建体引入棒状杆菌型细菌，培养由此转化的棒状杆菌型细菌，用蛋白酶等处理细胞外释放的蛋白质以切割和消除前体部分。
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公开(公告)号：US07704707B2
公开(公告)日：2010-04-27
申请号：US11218780
申请日：2005-09-06
CPC分类号： C12N9/1044 , C12N9/6489
摘要： The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.
摘要翻译： 本发明提供来自放线菌的中性金属蛋白酶，其选择性地切割微生物转谷氨酰胺酶的前体结构部分和编码所述中性金属蛋白酶的基因。 具有前体结构部分切割的活性微生物转谷氨酰胺酶可以通过培养从引入放线菌中产生中性金属蛋白酶的本发明的编码中性金属蛋白酶的基因的微生物获得，其中通过从放线菌产生中性金属蛋白酶， 微生物抗转谷氨酰胺酶。
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公开(公告)号：US20070184525A1
公开(公告)日：2007-08-09
申请号：US11550972
申请日：2006-10-19
申请人： Masayo Date , Yoshimi Kikuchi , Hiroshi Itaya , Nami Nakamura
发明人： Masayo Date , Yoshimi Kikuchi , Hiroshi Itaya , Nami Nakamura
摘要： The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5′-end to 3′-end, and secretory producing the heterologous protein by coryneform bacteria.
摘要翻译： 本发明提供了一种在棒状细菌中有效生产工业上有用的蛋白质的方法，更具体地，涉及使用常规的蛋白质分泌途径有效制备难以分泌的蛋白质的方法。 特别地，本发明提供了一种有效生产异源蛋白的方法，包括培养含有在棒状细菌中起作用的启动子序列的遗传构建的棒状细菌，编码Tat系统依赖性信号肽区的核酸序列和核酸 从5'端到3'端的方向编码异源蛋白的序列，以及通过棒状细菌产生异源蛋白的分泌。
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