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    Sample extraction chip and biological reaction device
    1.
    发明授权
    Sample extraction chip and biological reaction device 有权

    公开(公告)号：US11666911B2

    公开(公告)日：2023-06-06

    申请号：US17121337

    申请日：2020-12-14

    申请人： Capitalbio Corporation

    发明人： Longtang Zheng ,  Lei Wang ,  Liang Bai ,  Xinying Zhou ,  Fei Wen ,  Yanan Wang ,  Xiang Chen ,  Tengfei Guo ,  Baolian Li ,  Li Ma ,  Jing Cheng

    IPC分类号： B01L3/00

    CPC分类号： B01L3/502738 ,  B01L2300/0803

    摘要： A sample extraction chip and a biological reaction device are disclosed according to the present disclosure. The sample extraction chip includes a chip body and a sample extraction module provided on the chip body, the sample extraction module includes a sample-loading lysis unit, a liquid release-control unit, an extraction unit, a liquid switch-control unit, a liquid collection unit and a sample collection unit, which are connected through flow channels in a sequence of extraction. The liquid release-control unit is configured to store and release liquid reagents, and the liquid switch-control unit is configured to switch between communication of the liquid collection unit and the extraction unit and communication of the sample collection unit and the extraction unit. The sample collection unit includes a front collection portion and a rear collection portion which are both in communication with the liquid switch-control unit.

    AN INTEGRATED MICROFLUIDIC CHIP AND METHODS OF USE
    4.
    发明申请
    AN INTEGRATED MICROFLUIDIC CHIP AND METHODS OF USE 审中-公开

    公开(公告)号：US20190143324A1

    公开(公告)日：2019-05-16

    申请号：US16302081

    申请日：2017-05-18

    申请人： CAPITALBIO CORPORATION

    发明人： Lei WANG ,  Yao ZHOU ,  Wanli XING ,  Hu WANG ,  Jing CHENG ,  Xiang CHEN ,  Mingxian LIN

    IPC分类号： B01L3/00 ,  B01F13/00 ,  B01F15/02

    摘要： An integrated microfluidic chip, wherein at least one integrated reaction unit is provided on its substrate, and the integrated reaction unit comprises at least a sample cell (1), a mixing cell (2) and a reaction cell (3) connected through liquid channels (6). In one aspect, one end of the sample cell (1) is provided with a sample inlet (4), and the chip further comprises an internal air circulating system/circuit. One end of the internal air circulating system/circuit is connected with the mixing cell (2), while the other end comprises at least a first circulation branch circuit connected with the end of the sample cell (1) distal to the sample inlet (4).

    LUMINOPHORE-LABELED MOLECULES COUPLED WITH PARTICLES FOR MICROARRAY-BASED ASSAYS
    9.
    发明申请
    LUMINOPHORE-LABELED MOLECULES COUPLED WITH PARTICLES FOR MICROARRAY-BASED ASSAYS 审中-公开
    与基于微量元素测定的颗粒偶联的荧光标记分子

    公开(公告)号：US20160298179A1

    公开(公告)日：2016-10-13

    申请号：US15100970

    申请日：2014-12-02

    申请人： CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

    发明人： Yang LI ,  Guanbin ZHANG ,  Di JIANG ,  Guangxin XIANG ,  Wanli XING ,  Jing CHENG

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/58 ,  C12Q2537/143 ,  C12Q2563/103 ,  C12Q2563/149

    摘要： A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules.

    摘要翻译： 使用微阵列芯片标记与颗粒偶联用于检测靶分子的靶分子的方法，包括：提供官能化微粒，其中所述微粒涂覆有一个或多个官能团; 在待检测的每个靶分子上提供修饰基团以形成修饰的靶分子; 使官能化的微粒与修饰的靶分子接触; 将发光体耦合到功能化微粒和修饰的靶分子之间的复合物上，由此用发光体直接或间接标记每个修饰的靶分子。 通过用发光体直接或间接标记目标分子，该方法降低荧光检测的成本，并避免衍生自传统荧光标记分子的PCR抑制。

    FLUIDIC CHIP FOR FLOW CYTOMETRY AND METHODS OF USE
    10.
    发明申请
    FLUIDIC CHIP FOR FLOW CYTOMETRY AND METHODS OF USE 审中-公开
    用于流动细胞计数的流体芯片和使用方法

    公开(公告)号：US20160231223A1

    公开(公告)日：2016-08-11

    申请号：US15019715

    申请日：2016-02-09

    申请人： CapitalBio Corporation ,  Tsinghua University

    发明人： Dong WANG ,  Zhen CHENG ,  Danyang MEI ,  Dongliang AN ,  Wei YANG ,  Xiaokun QIN

    IPC分类号： G01N15/14 ,  B01L3/00

    CPC分类号： G01N15/1404 ,  B01L3/5027 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2400/0439 ,  B07C5/02 ,  B07C5/342 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/142 ,  G01N2015/149

    摘要： The present disclosure in some embodiments provides a flow cell sorting system, a focus detection method, as well as a fluidic chip. In one aspect, the fluidic chip comprises a cover sheet and one or more substrate layers, and one or more channels in each of the fluidic chips. In one aspect, the cells or other analytes in a sample are focused onto the same plane as they flow through the fluidic channel. In another aspect, the cells or other analytes on the same plane are irradiated with the same light intensity, for example, by a flat-top laser beam. This combination eliminates or reduces variation caused by cells at different positions receiving different intensities of the excitation light, and therefore improves accuracy of the flow cytometry analysis. In another aspect, a sorting apparatus is designed at the junction of the detection area and the outlet channel to separate the target cells.

    摘要翻译： 在一些实施例中的本公开提供了流动池分选系统，焦点检测方法以及流体芯片。 一方面，流体芯片包括覆盖片和一个或多个衬底层，以及每个流体碎片中的一个或多个通道。 在一个方面，样品中的细胞或其他分析物在流过流体通道时聚焦在相同的平面上。 在另一方面，例如通过平顶激光束以相同的光强照射同一平面上的细胞或其它分析物。 该组合消除或减少了在接收不同激发光强度的不同位置处由细胞引起的变化，因此提高了流式细胞术分析的准确性。 在另一方面，在检测区域和出口通道的连接处设计分选装置以分离靶细胞。