公开(公告)号：US08153764B2

公开(公告)日：2012-04-10

申请号：US12531430

申请日：2008-03-13

申请人： Ai Wakamatsu ,  Junichi Yamamoto ,  Takao Isogai

发明人： Ai Wakamatsu ,  Junichi Yamamoto ,  Takao Isogai

IPC分类号： C07K14/705 ,  C12N15/63

CPC分类号： G01N33/6896 ,  A61K38/00 ,  C07K14/47 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N2800/28

摘要： The invention provides a polypeptide and a specific partial peptide thereof, as well as a polynucleotide and a specific partial nucleotide thereof, that can be used as a biomarker specific for the brain/nerves or specific for nerve differentiation; an expression vector for such a polynucleotide and a specific partial peptide thereof; a transformant incorporating such an expression vector; an antisense molecule, RNAi-inducing nucleic acid (e.g., siRNA), aptamer, or antibody for such a biomarker, and a composition comprising the same; a mammalian cell or non-human mammal wherein the expression or a function of such a biomarker is regulated; a measuring means (e.g., primer set, nucleic acid probe, antibody, aptamer) for such a biomarker, and a reagent comprising the same and the like.

摘要翻译： 本发明提供了可用作脑/神经特异性或特异于神经分化的生物标志物的多肽及其特定部分肽以及其多核苷酸和特定的部分核苷酸; 这种多核苷酸及其特定部分肽的表达载体; 包含这种表达载体的转化体; 用于这种生物标志物的反义分子，RNAi诱导核酸（例如siRNA），适体或抗体，以及包含其的组合物; 哺乳动物细胞或非人哺乳动物，其中调节这种生物标志物的表达或功能; 用于这种生物标志物的测量装置（例如，引物组，核酸探针，抗体，适体）和包含该生物标记的试剂等。

公开(公告)号：US20110189789A1

公开(公告)日：2011-08-04

申请号：US12632905

申请日：2009-12-08

申请人： Akito Tanaka ,  Akira Yamazaki ,  Takeshi Tsutsumi ,  Tomohiro Terada ,  Masayuki Haramura

发明人： Akito Tanaka ,  Akira Yamazaki ,  Takeshi Tsutsumi ,  Tomohiro Terada ,  Masayuki Haramura

IPC分类号： G01N33/53

CPC分类号： C07D231/12 ,  A61K45/06 ,  C07C317/44 ,  C07C323/62

摘要： The present invention provides a pharmaceutical composition containing, as an active ingredient, a compound that specifically binds to KSRP or a functional fragment thereof, and a screening method for the compound. KSRP is a novel target protein for anticancer agents; a compound capable of regulating the expression and activity of such a protein and a pharmaceutical composition containing it are highly useful for proliferative diseases, particularly as anticancer agents. By providing the novel target protein, the mechanism behind the anticancer effect that has conventionally been unexplainable can be elucidated.

摘要翻译： 本发明提供含有与KSRP或其功能片段特异性结合的化合物作为活性成分的药物组合物和该化合物的筛选方法。 KSRP是抗癌剂的新型靶蛋白; 能够调节这种蛋白质的表达和活性的化合物和含有该蛋白质的药物组合物对于增殖性疾病，特别是作为抗癌剂是非常有用的。 通过提供新的靶蛋白，可以阐明通常不可解释的抗癌作用背后的机制。

公开(公告)号：US20100094026A1

公开(公告)日：2010-04-15

申请号：US12092208

申请日：2006-08-11

申请人： Akito Tanaka ,  Akinobu Nakanishi ,  Masayuki Haramura ,  Mikio Takeuchi

发明人： Akito Tanaka ,  Akinobu Nakanishi ,  Masayuki Haramura ,  Mikio Takeuchi

IPC分类号： A61K31/35 ,  C07D407/12 ,  G01N33/566

CPC分类号： G01N33/6893 ,  G01N2500/00 ,  G01N2800/24

摘要： The present invention provides a method of screening for a compound useful for treatment of an allergic disease by identifying a molecule targeted by Intal upon exertion of its efficacy as an anti-allergic agent (hereinafter referred as a target) and using the target, as well as a new type of anti-allergic agent comprising a compound obtainable by the screening as an active ingredient for treatment of an allergic disease.

摘要翻译： 本发明提供一种筛选用于治疗过敏性疾病的化合物的方法，其通过鉴定作为抗过敏剂（以下称为靶标）的功效的Intal作为靶标的分子，以及使用该靶标 作为新型的抗过敏剂，其包含通过筛选获得的化合物作为治疗过敏性疾病的活性成分。

公开(公告)号：US20130095107A1

公开(公告)日：2013-04-18

申请号：US13324610

申请日：2011-12-13

申请人： Ai WAKAMATSU ,  Junichi YAMAMOTO ,  Takao ISOGAI

发明人： Ai WAKAMATSU ,  Junichi YAMAMOTO ,  Takao ISOGAI

IPC分类号： A61K39/395 ,  C12N15/11 ,  C12N15/63 ,  C12N1/21 ,  A61P25/28 ,  C07K16/00 ,  C12N5/16 ,  A61K31/7088 ,  C12N1/19 ,  C12N5/10 ,  C07K2/00 ,  C07H21/00

CPC分类号： G01N33/6896 ,  A61K38/00 ,  C07K14/47 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N2800/28

摘要： The invention provides a novel polypeptide and a specific partial peptide thereof, as well as a novel polynucleotide and a specific partial nucleotide thereof, that can be used as a biomarker specific for the brain/nerves or specific for nerve differentiation; an expression vector for such a polynucleotide and a specific partial peptide thereof; a transformant incorporating such an expression vector; an antisense molecule, RNAi-inducing nucleic acid (e.g., siRNA), aptamer, or antibody for such a biomarker, and a composition comprising the same; a mammalian cell or non-human mammal wherein the expression or a function of such a biomarker is regulated; a measuring means (e.g., primer set, nucleic acid probe, antibody, aptamer) for such a biomarker, and a reagent comprising the same and the like.

摘要翻译： 本发明提供了一种新的多肽及其特定的部分肽，以及一种新的多核苷酸及其特定的部分核苷酸，可用作对脑/神经特异性或特异于神经分化的生物标志物; 这种多核苷酸及其特定部分肽的表达载体; 包含这种表达载体的转化体; 用于这种生物标志物的反义分子，RNAi诱导核酸（例如siRNA），适体或抗体，以及包含其的组合物; 哺乳动物细胞或非人哺乳动物，其中调节这种生物标志物的表达或功能; 用于这种生物标志物的测量装置（例如，引物组，核酸探针，抗体，适体）和包含该生物标记的试剂等。

公开(公告)号：US07754497B2

公开(公告)日：2010-07-13

申请号：US10569883

申请日：2004-08-24

申请人： Tsuyoshi Yamada ,  Yorimasa Suwa ,  Takeshi Tsutsumi

发明人： Tsuyoshi Yamada ,  Yorimasa Suwa ,  Takeshi Tsutsumi

IPC分类号： G01N33/543

CPC分类号： G01N33/543 ,  Y10T436/25 ,  Y10T436/255

摘要： This invention provides a method for immobilizing proteins comprising: step 1 of purifying target proteins to be immobilized, which have a first tag portion and a second tag portion, with the use of the first tag portion; step 2 of activating reactive groups capable of covalently binding to the proteins on a carrier for immobilization; and step 3 of allowing a solution containing the proteins purified in step 1 to react with the carrier after step 2, wherein, in step 3, the proteins are immobilized on the carrier via interactions between the second tag portion and the site of the carrier to which the second tag portion binds and via covalent binding between the reactive groups and the proteins. This method enables the stable immobilization of various types of target proteins on a carrier regardless of the amounts of target proteins and without nonspecific immobilization of contaminating proteins.

摘要翻译： 本发明提供了一种固定蛋白质的方法，包括：使用第一标签部分纯化具有第一标签部分和第二标签部分的待固定的靶蛋白质的步骤1; 启动能够共价结合载体上的蛋白质以进行固定的反应性基团的步骤2; 和步骤3，使步骤2中纯化的蛋白质溶液与载体反应，其中在步骤3中，蛋白质通过第二标签部分和载体位点之间的相互作用固定在载体上， 其中第二标签部分结合并且通过反应性基团和蛋白质之间的共价结合。 这种方法可以使各种类型的靶蛋白稳定地固定在载体上，而不管靶蛋白的量如何，并且不污染蛋白质的非特异性固定。

公开(公告)号：US20100136524A1

公开(公告)日：2010-06-03

申请号：US11577350

申请日：2005-10-18

申请人： Tadakazu Yamauchi ,  Hideaki Sueoka ,  Kouichi Tsuchiya ,  Katsuhisa Murayama ,  Kazuo Komiya ,  Morikazu Kito ,  Takeshi Tsutsumi ,  Yuko Isono ,  Motoi Tobita ,  Yorimasa Suwa

发明人： Tadakazu Yamauchi ,  Hideaki Sueoka ,  Kouichi Tsuchiya ,  Katsuhisa Murayama ,  Kazuo Komiya ,  Morikazu Kito ,  Takeshi Tsutsumi ,  Yuko Isono ,  Motoi Tobita ,  Yorimasa Suwa

IPC分类号： C12Q1/68 ,  C07D403/12 ,  C07D401/12 ,  C07K14/00 ,  C12N15/63

CPC分类号： C07D211/22 ,  C07D235/30 ,  C07D235/32 ,  C07D295/08 ,  C07D409/06 ,  C07D417/04 ,  G01N33/6893 ,  G01N2500/00

摘要： The present invention provides a novel target protein and a gene for drug discovery, and a means that enables development of a novel pharmaceutical agent by using the same. More particularly, the present invention provides CARP and genes thereof; screening methods for drugs (e.g., antiallergic drugs); a regulator of diseases (e.g., allergic diseases); a drug derivative production method; a complex comprising a drug and CARP, and a production method thereof; kits comprising a drug or a salt thereof; determination methods for the onset or risk of onset of a specified disease, determination methods for susceptibility to a drug, and determination kits used for said methods, and the like.

摘要翻译： 本发明提供了一种新的靶蛋白和用于药物发现的基因，并且是通过使用它们能够开发新型药剂的方法。 更具体地，本发明提供CARP及其基因; 药物筛选方法（如抗过敏药物）; 疾病调节剂（如过敏性疾病）; 药物衍生物生产方法; 包含药物和CARP的复合物及其制备方法; 包含药物或其盐的试剂盒; 确定特定疾病发病或发病风险的方法，对药物易感性的测定方法和用于所述方法的测定试剂盒等。

公开(公告)号：US20090156413A1

公开(公告)日：2009-06-18

申请号：US12320961

申请日：2009-02-10

申请人： Renpei Nagashima ,  Takao Isogai ,  Noriaki Hirayama

发明人： Renpei Nagashima ,  Takao Isogai ,  Noriaki Hirayama

IPC分类号： C40B30/00

CPC分类号： C40B30/04 ,  G01N33/6803 ,  G01N33/6845

摘要： Disclosed in this invention are methods, systems, databases, user-interfaces, software, media, and services useful for evaluating interactions between chemical compounds and proteins and for utilizing the information resulting from such evaluation for the purpose of discovering chemical compounds for medical and other fields. An approach termed “reverse proteomics” is disclosed. This invention generates an enormously large pool of new target proteins for drug discovery, novel methods for designing of new drugs, and a previously unthinkable pool of virtually synthesized small molecules for therapeutic uses. This invention is also applicable, for example, to discovery of substitutes for environmentally hazardous chemicals, more effective agrochemicals, and healthier food additives.

摘要翻译： 在本发明中公开的是用于评估化合物和蛋白质之间的相互作用的方法，系统，数据库，用户接口，软件，媒体和服务，以及为了发现用于医学和其它 领域。 公开了一种称为“反向蛋白质组学”的方法。 本发明产生了一大量用于药物发现的新靶蛋白，新的药物设计新方法和用于治疗用途的以前不可思议的几乎合成的小分子池。 本发明也可应用于例如发现环境危害化学品替代物，更有效的农用化学品和更健康的食品添加剂。

公开(公告)号：US07456022B2

公开(公告)日：2008-11-25

申请号：US11572972

申请日：2005-07-28

申请人： Masayuki Haramura ,  Akito Tanaka

发明人： Masayuki Haramura ,  Akito Tanaka

IPC分类号： B01J20/26 ,  G01N33/547

CPC分类号： B01J20/289 ,  B01J20/261 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  B01J2220/54 ,  G01N33/54353 ,  Y10T436/207497

摘要： The present invention provides a method of analyzing the interaction between a ligand and a target molecule, which uses a solid support wherein the ligand is immobilized via a linker cleavable by photoirradiation, particularly a method of searching and purifying a target molecule. By intervening a linker specifically cleavable by photoirradiation between the ligand molecule and the solid support, release and elution of the target molecule from the solid support without using a ligand or salt at a high concentration can be enabled, and suppression of release and elution of a nonspecific protein can be enabled.

摘要翻译： 本发明提供了一种分析配体和靶分子之间的相互作用的方法，其使用固体支持物，其中通过光照射可切割的接头固定配体，特别是搜索和纯化靶分子的方法。 通过插入通过配体分子和固体支持体之间的光照射可以特异性地切割的接头，可以使得目标分子从固体支持物中释放和洗脱而不使用高浓度的配体或盐，并且抑制释放和洗脱 可以启用非特异性蛋白质。

公开(公告)号：US20080102457A1

公开(公告)日：2008-05-01

申请号：US11718946

申请日：2005-11-09

申请人： Yorimasa Suwa ,  Tadakazu Yamauchi ,  Tsuyoshi Yamada ,  Hironori Oosaki ,  Morikazu Kito

发明人： Yorimasa Suwa ,  Tadakazu Yamauchi ,  Tsuyoshi Yamada ,  Hironori Oosaki ,  Morikazu Kito

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C07K16/00

CPC分类号： G01N33/5023 ,  G01N33/5008 ,  G01N33/502

摘要： The present invention provides target proteins and target genes for bioactive substances such as drugs, and means that enable the development of novel bioactive substances using the same. To be specific, the present invention provides target proteins and target genes for bioactive substances; screening methods for substances capable of regulating bioactivities; bioactivity regulators; a bioactive substance derivative production method; a complex comprising a bioactive substance and a target protein, and a method of producing the complex; and kits comprising a bioactive substance or a salt thereof; determination methods for the onset or risk of onset of a specified disease or condition, determination methods for susceptibility to a bioactive substance, and determination kits used for the determination methods, and the like.

摘要翻译： 本发明提供了用于生物活性物质如药物的靶蛋白和靶基因，并且使得能够开发使用其的新型生物活性物质的手段。 具体而言，本发明提供了用于生物活性物质的靶蛋白和靶基因; 能够调节生物活性的物质的筛选方法; 生物活性调节剂 生物活性物质衍生物生产方法; 包含生物活性物质和靶蛋白质的复合物，以及生产该复合物的方法; 和包含生物活性物质或其盐的试剂盒; 特定疾病或病症的发病或发病风险的确定方法，对生物活性物质易感性的测定方法和用于测定方法的测定试剂盒等。

公开(公告)号：US20090298954A1

公开(公告)日：2009-12-03

申请号：US12308151

申请日：2007-06-08

申请人： Ken Hosoya ,  Tomoko Mori ,  Akito Tanaka ,  Teruki Takahashi

发明人： Ken Hosoya ,  Tomoko Mori ,  Akito Tanaka ,  Teruki Takahashi

IPC分类号： A61K47/32

CPC分类号： B01J20/26 ,  B01J20/264 ,  B01J20/265 ,  B01J20/267 ,  C08F220/26 ,  C08F220/36 ,  C08F222/1006

摘要： An affinity carrier not of a particle aggregation type but in a gel state having a bicontinuous structure composed of pores having an average diameter of micrometer size arranged continuously in a three-dimensional network structure and a skeletal phase abundant in organic matters, comprisingat least one compound having at least two functionalities as a crosslinking agent of a vinyl monomer compound, a methacrylate compound or an acrylate compound; anda copolymer of at least one monofunctional hydrophilic monomer as a ligand-carrying monomer and at least one monofunctional thinning monomer as a thinning agent for arbitrarily controlling a hydrophilic/hydrophobic environment in which a ligand carried by said ligand-carrying monomer is put,wherein a volume ratio of said crosslinking agent to “said copolymer of the monofunctional hydrophilic monomer and the mono functional thinning monomer” in said affinity carrier is 100 to 10:0 to 90.

摘要翻译： 不具有粒子聚集型但凝胶状态的亲和载体，其具有由三维网状结构中连续排列的平均微米尺寸直径的孔和有机物丰富的骨架相构成的双连续结构，所述亲和载体包含至少一个 具有至少两种官能团的化合物作为乙烯基单体化合物，甲基丙烯酸酯化合物或丙烯酸酯化合物的交联剂; 和至少一种单官能亲水单体作为配体携带单体和至少一种单功能稀释单体的共聚物，作为用于任意控制所述配体携带单体携带的配位体的亲水/疏水环境的稀释剂，其中 所述亲和载体中所述交联剂与单功能亲水单体和单官能疏水单体的所述共聚物的体积比为100〜10：0〜90。