Method for the manufacture of a concentrate of a bifidogen factor
    2.
    发明授权
    Method for the manufacture of a concentrate of a bifidogen factor 失效
    制造双歧杆菌浓度的方法

    公开(公告)号:US3556801A

    公开(公告)日:1971-01-19

    申请号:US3556801D

    申请日:1967-01-18

    申请人: URSINA AG

    摘要: THIS INVENTION RELATES TO A METHOD FOR THE MANUFACTURE OF A BIFIDOGEN FACTOR CALLED "BIFIDUS 2" (BF2) WITH ENHANCED ACTIVITY. THE PROCESS OF THE INVENTION IS CHARACTERIZED BY PASSING A PROTEIN HYDROLYSATE CONTAINING THE FACTOR BF2 AT LEAST TWOCE THROUGH A CHROMATOGRAPHIC COLUMN OF AN ION-EXCHANGE RESIN OR THROUGH A DEXTRAN GEL. THE FIRST CHROMATOGRAPHY IS CHARACTERIZED BY PASSING THE HYDROLYSATE THROUGH A COLUMN OF A STRONG ACID CATION EXCHANGE RESIN IN THE ACID FORM. THE SECOND CHROMATOGRAPHY IS CHARACTERIZED BY PASSING THE CONCENTRATED ELUATE FROM THE FIRST CHROMATOGRAPHY PROCESS THROUGH A COLUMN OF A WEAK ACID CATION EXCHANGE RESIN. THE BF2 CONCENTRATE IS THEN OBTAINED BY A FURTHER CHROMATOGRAPHIC PROCESS USING A BASIC ANION EXCHANGE RESIN, OR FILTERING THE SOLUTION THROUGH GEL. THE PROCESS IS DIRECTED TOWARDS OBTAINING AN IMPROVEMENT IN THE YIELD OF BF2 CONCENTRATE FROM PROTEIN HYDROLYSATE SOLUTION.

    Chromatographic fractionation of whey
    9.
    发明授权
    Chromatographic fractionation of whey 失效
    乳清色谱分级

    公开(公告)号:US3969337A

    公开(公告)日:1976-07-13

    申请号:US455484

    申请日:1974-03-27

    摘要: The process for the chromatographic fractionation of whey comprising passing whey through a column of an ion exchange material, thereafter passing water through said column as eluant, and collecting the aqueous effluent from said column as a plurality of fractions. Advantageously the ion exchange material is a polystyrene resin containing sulfonic acid groups neutralized with calcium ions and cross-linked with about 2 to 4% by weight of divinyl-benzene, and the eluant is demineralized water.

    摘要翻译: 乳清色谱分级方法包括使乳清通过离子交换材料的柱,然后将水作为洗脱液通过所述柱,并以多种馏分收集来自所述塔的含水流出物。 有利地,离子交换材料是含有用钙离子中和的磺酸基的聚苯乙烯树脂,并与约2-4重量%的二乙烯基 - 苯进行交联,洗脱剂是软化水。