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14 条记录 (耗时 0.02 秒)

    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION
    1.
    发明申请
    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION 审中-公开
    DROPLET标记和放大的方法和系统

    公开(公告)号：US20170029813A1

    公开(公告)日：2017-02-02

    申请号：US15303893

    申请日：2015-04-17

    申请人： President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusets General Hospital

    发明人： David A. Weitz ,  Anthony John Iafrate ,  Zongli Zheng ,  Huidan Zhang

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    摘要： The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfiuidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid “tags,” and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

    摘要翻译： 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的方法和系统。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微液滴。 通过将多个核酸“标签”并入可选地扩增核酸，例如在液滴内，液滴暴露于的条件可由核酸编码。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。

    BARCODING SYSTEMS AND METHODS FOR GENE SEQUENCING AND OTHER APPLICATIONS
    2.
    发明公开
    BARCODING SYSTEMS AND METHODS FOR GENE SEQUENCING AND OTHER APPLICATIONS 审中-公开

    公开(公告)号：US20240043893A1

    公开(公告)日：2024-02-08

    申请号：US18353058

    申请日：2023-07-14

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Huidan Zhang ,  John Heyman ,  Allon Moshe Klein

    IPC分类号： C12P19/34 ,  C12Q1/6869 ,  C12N15/10

    CPC分类号： C12P19/34 ,  C12Q1/6869 ,  C12N15/1093 ,  C12N15/1006 ,  C12N15/1065 ,  C12Q2600/156

    摘要： The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets or other compartments, for instance, arising from a cell. In one set of embodiments, particles may be prepared containing oligonucleotides that can be used to determine target nucleic acids, e.g., attached to the surface of the particles. The oligonucleotides may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together or removed from the droplets. Certain embodiments of the invention are generally directed to systems and methods for attaching additional or arbitrary sequences to the nucleic acids within microfluidic droplets or other compartments, e.g., recognition sequences that can be used to selectively determine or amplify a desired sequence suspected of being present within a droplet. Such systems may be useful, for example, for selective amplification in various applications, such as high-throughput sequencing applications.

    DETERMINATION OF RNA IN BLOOD OR OTHER FLUIDS
    3.
    发明申请
    DETERMINATION OF RNA IN BLOOD OR OTHER FLUIDS 审中-公开

    公开(公告)号：US20190153427A1

    公开(公告)日：2019-05-23

    申请号：US16315194

    申请日：2017-07-07

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Huidan Zhang ,  Nai Wen Cui

    IPC分类号： C12N15/10 ,  C01C1/24 ,  C01C1/14 ,  C12N9/22

    摘要： The present invention generally relates to systems and methods for determining RNA in blood or other fluids. In certain embodiments, blood or other fluids may be treated to isolate or separate RNA, for example, from DNA, cells, and other material. In some cases, the RNA may arise from bacteria or other pathogens or foreign organisms that may be found within the blood or other fluid. In some cases, RNA stabilizing reagents, such as ammonium sulfate, may be added to stabilize RNA, then cells within the blood may be lysed to release the RNA (and other materials) from the cells, thereby producing a lysate. The lysate may be treated, e.g., to separate nucleic acids from other components within the lysate, and in some cases, DNA may be degraded, e.g., using DNAses or other suitable enzymes, leaving behind the RNA. The RNA can then be studied, purified, analyzed, amplified, stored, or the like.

    MICROFLUIDIC SEQUENCING TECHNIQUES
    5.
    发明申请
    MICROFLUIDIC SEQUENCING TECHNIQUES 审中-公开

    公开(公告)号：US20190185800A1

    公开(公告)日：2019-06-20

    申请号：US16319196

    申请日：2017-07-20

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Huidan Zhang ,  Nai Wen Cui

    IPC分类号： C12M3/06 ,  C12Q1/6858 ,  C12M1/00 ,  C12Q1/6806

    CPC分类号： C12M23/16 ,  C12M3/00 ,  C12M47/06 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q2521/301 ,  C12Q2525/131 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2565/629

    摘要： The present invention generally relates to microfluidics and, in some embodiments, to the determination of cells. In some aspects, primers able to introduce restriction sites into certain amplified nucleic acids are used. For example, the primers may introduce restriction sites into normal (wild-type) nucleic acids, but be unable to introduce restriction sites into mutant nucleic acids, e.g., due to a mismatch in the nucleic acid sequences caused by the mutant. After amplification, the nucleic acids may be exposed to a suitable restriction enzyme, which may cleave normal nucleic acids but not the mutant nucleic acids. In this way, mutant nucleic acids may be relatively quickly identified. In some embodiments, cells may be contained within microfluidic droplets and assayed to determine the mutant cells. In certain cases, for example, the nucleic acids may be amplified within droplets and attached to suitable tags, e.g., prior to breaking or merging the droplets and sequencing of the nucleic acids.

    SYSTEMS, METHODS, AND KITS FOR AMPLIFYING OR CLONING WITHIN DROPLETS
    6.
    发明申请
    SYSTEMS, METHODS, AND KITS FOR AMPLIFYING OR CLONING WITHIN DROPLETS 审中-公开

    公开(公告)号：US20180016622A1

    公开(公告)日：2018-01-18

    申请号：US15544942

    申请日：2016-01-22

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  John Heyman ,  Huidan Zhang ,  Linas Mazutis

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00

    摘要： The present invention generally relates to droplet-based microfluidic devices, including systems, methods, and kits for amplifying or cloning within droplets. In some embodiments, the present invention is generally directed to systems, methods, or kits for amplifying a plurality of nucleic acids, e.g., without substantially selectively amplifying some nucleic acids over others. The nucleic acids may be contained within the droplets. In addition, in some embodiments, a plurality of microfluidic droplet containing a species of interest, such as a nucleic acid, may be mixed with microfluidic droplets free of the species, then pipetted or otherwise transferred such that, on average, a predetermined number of droplets containing species of interest is transferred.

    DETERMINATION OF CELLS USING AMPLIFICATION
    9.
    发明申请
    DETERMINATION OF CELLS USING AMPLIFICATION 有权

    公开(公告)号：US20210254129A1

    公开(公告)日：2021-08-19

    申请号：US16953547

    申请日：2020-11-20

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Huidan Zhang

    IPC分类号： C12Q1/686 ,  C12Q1/689 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6886

    摘要： The present invention generally relates to microfluidics and, in particular, to systems and methods for determining cells using amplification. In one set of embodiments, cells are encapsulated within droplets and nucleic acids from the cells amplified within the droplets. The droplets may then be pooled together and the amplified nucleic acids can be determined using PCR or other suitable techniques. In some embodiments, techniques such as these can be used to detect relatively rare cells that may be present, e.g., if the droplets are amplified using conditions able to selectively amplify nucleic acids arising from the relatively rare cells.

    DEVICES AND METHODS FOR DETERMINING NUCLEIC ACIDS USING DIGITAL DROPLET PCR AND RELATED TECHNIQUES
    10.
    发明申请
    DEVICES AND METHODS FOR DETERMINING NUCLEIC ACIDS USING DIGITAL DROPLET PCR AND RELATED TECHNIQUES 有权

    公开(公告)号：US20230045126A1

    公开(公告)日：2023-02-09

    申请号：US17790450

    申请日：2021-01-13

    申请人： President and Fellows of Harvard College

    发明人： David A. Weitz ,  Huidan Zhang

    IPC分类号： C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/686 ,  B01L3/00 ,  C12Q1/6834

    摘要： The present disclosure generally relates, in certain aspects, to droplet-based microfluidic devices and methods. In certain aspects, target nucleic acids contained within droplets are amplified within droplets in a first step, where multiple primers may be present. However, multiple primers may cause multiple target nucleic acids to be amplified within the droplets, which can make it difficult to identify which nucleic acids were amplified. In a second step, the amplified nucleic acids may be determined. For example, the droplets may be broken and the amplified nucleic acids can be pooled together and sequenced. As an example, new droplets may be formed containing the amplified nucleic acids, and those nucleic acids within the droplets amplified by exposure to certain primers.