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    NOVEL EXPRESSION VECTOR
    3.
    发明申请
    NOVEL EXPRESSION VECTOR 有权
    新的表达向导

    公开(公告)号：US20150337331A1

    公开(公告)日：2015-11-26

    申请号：US14396929

    申请日：2013-04-25

    申请人： JCR Pharmaceuticals Co., Ltd.

    发明人： Kenichi Takahashi ,  Shinji Kakimoto

    IPC分类号： C12N15/85 ,  C12N9/00 ,  C07K16/28 ,  C07K14/59 ,  C07K14/505 ,  C12N15/52 ,  C12N9/06

    CPC分类号： C12N15/85 ,  C07K14/505 ,  C07K14/59 ,  C07K16/2887 ,  C07K2317/14 ,  C12N9/003 ,  C12N9/93 ,  C12N15/52 ,  C12N2800/107 ,  C12N2830/00 ,  C12N2830/42 ,  C12N2830/50 ,  C12N2830/60 ,  C12N2840/60 ,  C12Y105/01003 ,  C12Y603/01002

    摘要： Disclosed are a novel expression vector for efficient expression of recombinant proteins in mammalian cells, a mammalian cell transformed with the vector, and a method for production of the mammalian cell. The expression vector is an expression vector for expression of a mammalian protein and includes a gene expression regulatory site, and a gene encoding the protein downstream thereof, and an internal ribosome entry site further downstream thereof, and a gene encoding a glutamine synthetase further downstream thereof, and a dihydrofolate reductase gene downstream of either the same gene expression regulatory site or another gene expression regulatory site in addition to the former.

    摘要翻译： 公开了一种用于在哺乳动物细胞中有效表达重组蛋白的新型表达载体，用载体转化的哺乳动物细胞，以及哺乳动物细胞的制备方法。 表达载体是用于表达哺乳动物蛋白质的表达载体，包括基因表达调节位点和编码其下游蛋白的基因，以及其下游的内部​​核糖体进入位点，以及编码其下游的谷氨酰胺合成酶的基因 ，以及除了前者之外的相同基因表达调节位点或另一基因表达调节位点下游的二氢叶酸还原酶基因。

    Puro-DHFR quadrifunctional marker and its use in protein production
    4.
    发明授权
    Puro-DHFR quadrifunctional marker and its use in protein production 有权
    Puro-DHFR四官能标记及其在蛋白质生产中的应用

    公开(公告)号：US09175284B2

    公开(公告)日：2015-11-03

    申请号：US14481983

    申请日：2014-09-10

    申请人： MERCK SERONO SA

    发明人： Michel Kobr ,  Philippe Dupraz

    IPC分类号： C12N15/63 ,  C12N15/85 ,  C12N9/96 ,  G01N33/74

    CPC分类号： C12N9/96 ,  C12N2510/02 ,  C12Y105/01003 ,  C12Y203/01005 ,  G01N33/74 ,  G01N2333/90666

    摘要： This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).

    摘要翻译： 本发明涉及蛋白质的工业化生产。 更具体地，本发明涉及res-DHFR替代标记，其对应于DHFR与赋予对有毒化合物的抗性或赋予代谢优势的蛋白质之间的融合。 本发明还涉及res-DHFR用于筛选高表达目的蛋白质的细胞的用途。 本发明由Puro-DHFR替代标记说明，其对应于嘌呤霉素N-乙酰转移酶和二氢叶酸还原酶（DHFR）之间的融合物。

    Methods and Products for Producing Engineered Mammalian Cell Lines With Amplified Transgenes
    5.
    发明申请
    Methods and Products for Producing Engineered Mammalian Cell Lines With Amplified Transgenes 审中-公开
    用于产生具有扩增转基因的工程哺乳动物细胞系的方法和产品

    公开(公告)号：US20140179005A1

    公开(公告)日：2014-06-26

    申请号：US14091572

    申请日：2013-11-27

    申请人： Precision Biosciences, Inc.

    发明人： Derek JANTZ ,  James Jefferson SMITH ,  Michael G. NICHOLSON

    IPC分类号： C12N15/90 ,  C12N9/22

    CPC分类号： C12N15/907 ,  C12N9/003 ,  C12N9/22 ,  C12N9/93 ,  C12Y105/01003 ,  C12Y301/04 ,  C12Y603/01002

    摘要： Methods of inserting genes into defined locations in the chromosomal DNA of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest. The invention also relates to meganucleases, vectors and engineered cell lines necessary for performing the methods, to cell lines resulting from the application of the methods, and use of the cell lines to produce protein products of interest.

    摘要翻译： 公开了将基因插入受基因扩增的培养的哺乳动物细胞系的染色体DNA中的限定位置的方法。 特别地，将感兴趣的序列（例如，编码生物治疗性蛋白的基因）插入到可扩增位点中的可选择基因的近端，并且对转化的细胞进行选择以诱导可选择基因和感兴趣的序列的共扩增。 本发明还涉及实施方法所需的大范围核酸酶，载体和工程细胞系，应用所述方法产生的细胞系，以及细胞系用于产生感兴趣的蛋白质产物的用途。