利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.016 秒)

    Clinical disorders associated with carboxypeptidase E mutation
    1.
    发明授权
    Clinical disorders associated with carboxypeptidase E mutation 失效
    与羧肽酶E突变相关的临床疾病

    公开(公告)号：US5593837A

    公开(公告)日：1997-01-14

    申请号：US452262

    申请日：1995-05-26

    申请人： J urgen K. Naggert ,  Edward H. Leiter

    发明人： J urgen K. Naggert ,  Edward H. Leiter

    IPC分类号： A61K38/00 ,  C12N9/48 ,  C12G1/68

    CPC分类号： C12N9/48 ,  A61K38/00 ,  Y10S436/811

    摘要： Disclosed herein is a therapeutic method for treating a clinical disorder associated with a mutation in the carboxypeptidase E gene. In the therapeutic method of the invention, a molecule having carboxypeptidase activity is introduced into the plasma of the individual being treated. Such molecules include, for example, carboxypeptidase H, carboxypeptidase M, carboxypeptidase N, carboxypeptidase U and carboxypeptidase B. Also disclosed are methods for identifying individuals falling within the class for which the therapeutic method described above can be effective. These methods include, for example, the isolation of DNA encoding carboxypeptidase E followed by either: 1) sequence determination and comparison to wild-type; or 2) expression and comparison of activity to wild-type activity. Also disclosed are oligonucleotide probes useful for diagnosing a clinical disorder such as obesity, impaired glucose tolerance and diabetes in an individual, the clinical disorder being associated with a mutation in the carboxypeptidase E gene.

    摘要翻译： 本文公开了治疗与羧肽酶E基因突变相关的临床病症的治疗方法。 在本发明的治疗方法中，将具有羧肽酶活性的分子引入待治疗的个体的血浆中。 这样的分子包括例如羧肽酶H，羧肽酶M，羧肽酶N，羧肽酶U和羧肽酶B.还公开了鉴定属于上述治疗方法有效的类别内的个体的方法。 这些方法包括例如分离编码羧肽酶E的DNA，之后是：1）序列测定和与野生型的比较; 或2）活性与野生型活性的表达和比较。 还公开了可用于诊断临床病症如肥胖，糖耐量异常和个体糖尿病的寡核苷酸探针，所述临床病症与羧肽酶E基因中的突变有关。

    Enzyme-amplified lanthanide chelate luminescence
    3.
    发明授权
    Enzyme-amplified lanthanide chelate luminescence 失效
    酶扩增镧系元素螯合发光

    公开(公告)号：US5262299A

    公开(公告)日：1993-11-16

    申请号：US612171

    申请日：1990-11-13

    申请人： Ramon A. Evangelista ,  Eva F. G. Templeton ,  Alfred Pollak

    发明人： Ramon A. Evangelista ,  Eva F. G. Templeton ,  Alfred Pollak

    IPC分类号： C12Q1/26 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58 ,  C12G1/68

    CPC分类号： G01N33/542 ,  C12Q1/26 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/40 ,  Y10S435/968 ,  Y10S436/80 ,  Y10T436/13

    摘要： A method and compounds useful for this method are described for enzyme-amplified signal detection in analytical assays requiring extremely high detection sensitivity which uses a substrate capable of being transformed by an enzyme from a compound which does not form a luminescent lanthanide chelate into a product which forms a luminescent lanthanide chelate. The method in which a substrate not capable of forming a highly luminescent lanthanide chelate is enzymatically altered to produce a product which forms a highly luminescent lanthanide chelate and hence is particularly useful in time-resolved luminescence analysis as required in many different heterogeneous or homogeneous assay formats is described herein.

    摘要翻译： 描述了用于该方法的方法和化合物，用于在需要极高检测灵敏度的分析测定中进行酶扩增信号检测，其使用能够被酶从不形成发光镧系螯合物的化合物转化成产物的底物 形成发光的镧系元素螯合物。 不能形成高度发光的镧系元素螯合物的底物被酶促改变以产生形成高度发光的镧系元素螯合物的产物的方法，因此特别可用于许多不同的异构或均相测定形式所要求的时间分辨发光分析 在此描述。

    Clinical disorders associated with carboxypeptidase E mutation
    4.
    发明授权
    Clinical disorders associated with carboxypeptidase E mutation 失效

    公开(公告)号：US5690932A

    公开(公告)日：1997-11-25

    申请号：US734550

    申请日：1996-10-17

    申请人： Jurgen K. Naggert ,  Edward H. Leiter

    发明人： Jurgen K. Naggert ,  Edward H. Leiter

    IPC分类号： A61K38/00 ,  C12N9/48 ,  A61K38/46 ,  C12G1/68

    CPC分类号： C12N9/48 ,  A61K38/00 ,  Y10S436/811

    摘要： Disclosed herein is a therapeutic method for treating a clinical disorder associated with a mutation in the carboxypeptidase E gene. In the therapeutic method of the invention, a molecule having carboxypeptidase activity is introduced into the plasma of the individual being treated. Such molecules include, for example, carboxypeptidase H, carboxypeptidase M, carboxypeptidase N, carboxypeptidase U and carboxypeptidase B. Also disclosed are methods for identifying individuals falling within the class for which the therapeutic method described above can be effective. These methods include, for example, the isolation of DNA encoding carboxypeptidase E followed by either: 1) sequence determination and comparison to wild-type; or 2) expression and comparison of activity to wild-type activity. Also disclosed are oligonucleotide probes useful for diagnosing a clinical disorder such as obesity, impaired glucose tolerance and diabetes in an individual, the clinical disorder being associated with a mutation in the carboxypeptidase E gene.

    Process for the characterization of human rhinoviruses
    5.
    发明授权
    Process for the characterization of human rhinoviruses 失效
    人类鼻病毒表征的过程

    公开(公告)号：US5340713A

    公开(公告)日：1994-08-23

    申请号：US541907

    申请日：1990-06-22

    申请人： Helge Torgersen ,  Timothy Skern ,  Dieter Blaas

    发明人： Helge Torgersen ,  Timothy Skern ,  Dieter Blaas

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12G1/70 ,  C07H17/00 ,  C12P19/34 ,  C12G1/68

    CPC分类号： C12Q1/701 ,  C12Q1/683 ,  C12Q1/686

    摘要： The present invention relates to a method of characterizing viruses and the use thereof for classification. According to the invention, a cDNA copy of a first virus is made and the cDNA is amplified and cleaved using at least one restriction endonuclease. The location of restriction fragments is mapped and compared to that of other viruses. Primers useful for amplifying the cDNA may be derived from sequences found in the 5' non-coding regions of picornavirus including human rhinovirus.

    摘要翻译： 本发明涉及一种表征病毒的方法及其用于分类的用途。 根据本发明，制备第一病毒的cDNA拷贝，并使用至少一种限制性内切核酸酶扩增和切割cDNA。 限制片段的位置被映射并与其他病毒的位置进行比较。 用于扩增cDNA的引物可以衍生自包括人鼻病毒在内的小核糖核酸病毒5'非编码区的序列。

    Nucleotide probe for Neisseria gonrrhoeae
    6.
    发明授权
    Nucleotide probe for Neisseria gonrrhoeae 失效
    奈瑟氏球菌的核苷酸探针

    公开(公告)号：US5256536A

    公开(公告)日：1993-10-26

    申请号：US611528

    申请日：1990-11-09

    申请人： Charles G. Miyada ,  Teresa L. Born

    发明人： Charles G. Miyada ,  Teresa L. Born

    IPC分类号： C07K7/06 ,  C07K16/12 ,  C12N15/31 ,  C12Q1/68 ,  C12G1/68 ,  C07M15/12 ,  C12D12/34 ,  C12Q1/10

    CPC分类号： C12Q1/689 ,  Y10S435/822 ,  Y10S435/871

    摘要： A nucleotide sequence characteristic of Neisseria gonorrhoeae is disclosed. The sequence can be the basis for hybridization type, nucleic acid-based, rapid, in vitro diagnostic assays. The unique nature of the sequence makes it possible to clearly discriminate N. gonorrhoeae from other Neisseria species thus eliminating or substantially reducing the number of false positive readings. A 350 base pair N. gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. In further cloning experiments the sequences adjacent to the original 350 base pair fragment were determined. A portion of this sequence was shown to detect 105 of 106 N. gonorrhoeae strains and no other Neisseria species. In addition to use as detection probes, all or portions of the nucleotide sequence can be used as a ligand for the sandwich capture of N. gonorrhoeae sequences and as primers for in vitro amplification of N. gonorrhoeae sequences. The polypeptides encoded by the presently disclosed sequence, including antibodies thereto, are also disclosed as are their uses.

    摘要翻译： 披露了淋病奈瑟氏球菌特征的核苷酸序列。 该序列可以是杂交类型，基于核酸，快速，体外诊断测定的基础。 序列的独特性质使得可以清楚地区分淋病奈瑟氏球菌与其他奈瑟氏菌属物种，从而消除或显着减少假阳性读数的数量。 在与奈瑟氏球菌脑膜炎奈瑟菌DNA消减杂交后克隆了350碱基对淋病奈瑟氏球菌DNA限制性片段。 在进一步克隆实验中，确定与原始350碱基对片段相邻的序列。 该序列的一部分显示检测到106个淋病奈瑟氏球菌菌株中的105个，并且没有其他的奈瑟球菌属物种。 除了用作检测探针之外，核苷酸序列的全部或部分可以用作奈氏淋病奈瑟氏球菌序列的夹心捕获的配体，并且用作用于体外扩增淋病奈瑟氏球菌序列的引物。 本文公开的序列编码的多肽（包括其抗体）也被用于其用途。