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13 条记录 (耗时 0.015 秒)

    Cationic liposomes containing immune response generating moieties
    1.
    发明授权
    Cationic liposomes containing immune response generating moieties 有权
    含有免疫应答产生部分的阳离子脂质体

    公开(公告)号：US07790696B2

    公开(公告)日：2010-09-07

    申请号：US12101107

    申请日：2008-04-10

    申请人： Gregory Gregoriadis

    发明人： Gregory Gregoriadis

    IPC分类号： A61K31/70 ,  A61K9/127 ,  C12P21/06 ,  C12N21/00

    CPC分类号： C12N15/88 ,  A61K9/1272 ,  A61K48/00 ,  Y10S977/803 ,  Y10S977/904 ,  Y10S977/907 ,  Y10S977/917 ,  Y10S977/918

    摘要： Cationic liposomes with entrapped polynucleotide in the intravesicular space are described. The liposomes include cationic components such as cationic lipids such as DOTAP. Preferably the method of forming liposomes uses the dehydration-rehydration method in the presence of the polynucleotide. The polynucleotide preferably operatively encodes an antigen capable of eliciting a desired immune response, that is, is a gene vaccine.

    摘要翻译： 描述了在囊内空间内具有夹带多核苷酸的阳离子脂质体。 脂质体包括阳离子组分如阳离子脂质如DOTAP。 优选地，形成脂质体的方法在多核苷酸的存在下使用脱水 - 再水化方法。 多核苷酸优选有效地编码能够引发所需免疫应答的抗原，即基因疫苗。

    Compositions and methods for inhibiting expression of a gene from the JC virus
    5.
    发明授权
    Compositions and methods for inhibiting expression of a gene from the JC virus 失效
    用于抑制基因从JC病毒表达的组合物和方法

    公开(公告)号：US07691824B2

    公开(公告)日：2010-04-06

    申请号：US11741205

    申请日：2007-04-27

    申请人： Pamela Tan ,  Dinah Sah ,  Birgit Bramlage

    发明人： Pamela Tan ,  Dinah Sah ,  Birgit Bramlage

    IPC分类号： A61K31/70 ,  C07H21/04 ,  C12N21/00 ,  C12N15/00 ,  C12N5/00

    CPC分类号： C12N15/1131 ,  A61K48/005 ,  C07H21/02 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3521

    摘要： The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a gene from the JC Virus (JC virus genome), comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of a gene from the JC Virus. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by JC virus expression and the expression of a gene from the JC Virus using the pharmaceutical composition; and methods for inhibiting the expression of a gene from the JC Virus in a cell.

    摘要翻译： 本发明涉及用于抑制来自JC病毒（JC病毒基因组）的基因表达的双链核糖核酸（dsRNA），其包含长度小于30个核苷酸的核苷酸序列的反义链，通常为19- 长度为25个核苷酸，并且其与来自JC病毒的基因的至少一部分基本互补。 本发明还涉及包含dsRNA与药学上可接受的载体的药物组合物; 用于治疗由JC病毒表达引起的疾病的方法和使用该药物组合物从JC病毒中表达基因的方法; 以及用于抑制细胞中来自JC病毒的基因表达的方法。

    Liposomes
    6.
    发明授权
    Liposomes 有权
    脂质体

    公开(公告)号：US07384923B2

    公开(公告)日：2008-06-10

    申请号：US10617734

    申请日：2003-07-14

    申请人： Gregory Gregoriadis

    发明人： Gregory Gregoriadis

    IPC分类号： A61K31/70 ,  A61K9/127 ,  C12P21/06 ,  C12N21/00

    CPC分类号： C12N15/88 ,  A61K9/1272 ,  A61K2039/53

    摘要： Cationic liposomes with entrapped polynucleotide in the intravesicular space are described. The liposomes include cationic components such as cationic lipids such as DOTAP. Preferably the method of forming liposomes uses the dehydration-rehydration method in the presence of the polynucleotide. The polynucleotide preferably operatively encodes an antigen capable of eliciting a desired immune response, that is, is a gene vaccine.

    摘要翻译： 描述了在囊内空间内具有夹带多核苷酸的阳离子脂质体。 脂质体包括阳离子组分如阳离子脂质如DOTAP。 优选地，形成脂质体的方法在多核苷酸的存在下使用脱水 - 再水化方法。 多核苷酸优选有效地编码能够引发所需免疫应答的抗原，即基因疫苗。

    Increased wax production in plants
    7.
    发明授权
    Increased wax production in plants 有权
    增加植物中的蜡产量

    公开(公告)号：US07253342B2

    公开(公告)日：2007-08-07

    申请号：US11253300

    申请日：2005-10-18

    申请人： Jiyi Zhang ,  Zengyu Wang

    发明人： Jiyi Zhang ,  Zengyu Wang

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  C12N21/00 ,  C12N15/29

    CPC分类号： C12N15/8247 ,  C07K14/415 ,  C12N15/8273

    摘要： The invention provides genes that activate wax biosynthesis in plants. Also provided are constructs comprising these sequences, plants transformed therewith and methods of use thereof. The invention allows the modification of plants for increased wax production, particularly in leaves. The inventors have demonstrated increased drought tolerance in connection with increased leaf wax production engineered in plants.

    摘要翻译： 本发明提供了在植物中活化蜡生物合成的基因。 还提供了包含这些序列的构建体，用其转化的植物及其使用方法。 本发明允许改变植物以增加蜡产生，特别是在叶中。 本发明人已经证明，与在植物中工程化的叶蜡生产增加有关的耐旱性增加。

    Method for cloning of a rare, specifically mutated cell
    9.
    发明授权
    Method for cloning of a rare, specifically mutated cell 失效
    用于克隆罕见的，特异性突变的细胞的方法

    公开(公告)号：US06929917B2

    公开(公告)日：2005-08-16

    申请号：US10298850

    申请日：2002-11-18

    申请人： Richard Metz ,  Mike DiCola ,  R. Michael Blaese

    发明人： Richard Metz ,  Mike DiCola ,  R. Michael Blaese

    IPC分类号： C12N20060101 ,  C12N15/00 ,  C12N15/09 ,  C12N15/10 ,  C12N15/85 ,  C12Q1/68 ,  C12N21/00

    CPC分类号： C12N15/1034

    摘要： The invention concerns a new method of detecting a rare product of a directed genetic alteration of a cultured cell. The method is applicable to any method of making the alteration provided that a pair of closely linked alterations can be made. The method consists of sequentially using allele specific polymerase chain reaction (PCR) to preferentially amplify sequences containing one of the two linked alterations coupled with a second method that detects the second change in the PCR product. The second method can be restriction digestion, traditional sequencing or pyro-sequencing. Experiments indicate that alterations as rare as one correctly altered copy in 10,000 cells can be detected.

    摘要翻译： 本发明涉及一种检测培养细胞的定向遗传改变的稀有产物的新方法。 该方法适用于任何进行变更的方法，条件是可以进行一对紧密连接的变更。 该方法由顺序地使用等位基因特异性聚合酶链反应（PCR）优先扩增含有两个连接的改变之一的序列，并与检测PCR产物的第二个变化的第二种方法相结合。 第二种方法可以是限制性消化，传统测序或热解测序。 实验表明，可以检测到10,000个细胞中一个正确改变的拷贝稀少的改变。