公开(公告)号：US11596876B2

公开(公告)日：2023-03-07

申请号：US16267579

申请日：2019-02-05

申请人： CLEMSON UNIVERSITY RESEARCH FOUNDATION

发明人： R. Kenneth Marcus ,  Terri F. Bruce ,  Lei Wang ,  Sisi Huang ,  Tyler Y. Slonecki ,  Rhonda Reigers Powell

IPC分类号： B01D15/32 ,  G01N30/00 ,  C07K14/47 ,  C12N9/00 ,  C12N15/10 ,  G01N1/40 ,  G01N33/50 ,  A61K35/68 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/286

摘要： A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biologically active nanoparticles from other components of a mixture. Biologically active nanoparticles can include natural nanoparticles (e.g., exosomes, lysosomes, virus particles) as well as synthetic nanoparticles (liposomes, genetically modified virus particles, etc.)

公开(公告)号：US20230060770A1

公开(公告)日：2023-03-02

申请号：US17821120

申请日：2022-08-19

申请人： Cephalon LLC

发明人： Lu WANG ,  Albert KAO ,  Zhaoqing ZHANG ,  Mi JIN ,  Tianyi ZHOU

IPC分类号： B01J20/286 ,  C07K1/22 ,  C07K16/18 ,  B01D15/36 ,  B01J20/28 ,  C12Q1/686 ,  C07K1/18 ,  C07K16/00 ,  C07K16/28

摘要： A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations.

公开(公告)号：US20220339628A1

公开(公告)日：2022-10-27

申请号：US17753707

申请日：2020-09-07

申请人： COMMISSARIAT À L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES ALTERNATIVES ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNIVERSITE PARIS-SACLAY

发明人： Carole Bresson ,  Marta Garcia-Cortes ,  Claude Vidaud ,  Thuy Tran

IPC分类号： B01L3/00 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  C07K1/04 ,  C01G43/025

摘要： A method for preparing, in the internal volume of at least one channel, a monolithic support on which uranyl cations are immobilised. The method comprises: (a) activating the inner surface of the channel(s); (b) introducing, into the internal volume of the channel(s), a polymerisation solution comprising: a monomer comprising a phosphate group, at least one crosslinking agent, several solvents, and a radical polymerisation initiator; (c) polymerising the polymerisation solution; (d) rinsing the monolithic support obtained in step (c); and (e) contacting the monolithic support previously rinsed, with a solution comprising uranyl cations. A method for capturing proteins that selectively bind uranium by means of a monolithic support prepared by the above-mentioned method, as well as to a method for recovering proteins that selectively bind uranium with the capture method.

公开(公告)号：US11426706B2

公开(公告)日：2022-08-30

申请号：US16014778

申请日：2018-06-21

申请人： Cephalon, Inc.

发明人： Lu Wang ,  Albert Kao ,  Zhaoqing Zhang ,  Mi Jin ,  Tianyi Zhou

IPC分类号： B01D15/36 ,  C07K1/18 ,  B01J20/286 ,  B01J20/28 ,  C07K1/22 ,  C07K16/18 ,  C12Q1/686 ,  C07K16/00 ,  C07K16/28

摘要： A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations.

公开(公告)号：US20220034852A1

公开(公告)日：2022-02-03

申请号：US17370589

申请日：2021-07-08

申请人： Waters Technologies Corporation

发明人： Matthew A. Lauber ,  Stephan M. Koza ,  Pamela C. Iraneta ,  Kevin D. Wyndham

IPC分类号： G01N30/06 ,  G01N30/72 ,  C08F230/08 ,  B01D15/30 ,  C08L33/14 ,  C08F220/54 ,  C08F220/56 ,  B01J20/28 ,  B01J20/32 ,  C08F222/38 ,  B01J20/286

摘要： The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

公开(公告)号：US20210355098A1

公开(公告)日：2021-11-18

申请号：US17173193

申请日：2019-08-12

申请人： HURON BOTANICALS, LLC

发明人： Paul J. Kerry ,  Brad J. Maurer

IPC分类号： C07D311/80 ,  B01J20/10 ,  B01J20/18 ,  B01J20/14 ,  B01J20/28 ,  B01J20/286 ,  B01J20/283 ,  C11B7/00 ,  B01D11/02 ,  B01D39/06

摘要： A closed loop extraction process contains a filter assembly 20 for vacuum filtration of an extraction solvent 50 after it has extracted desired constituents from a plant material. High purity products formed from the process are also provided. A closed loop extraction system 10 contains a filter assembly 20 for filtering an extraction solvent 50 and extract prior to collection of desired products within a collection vessel 34. A filter assembly 20, used in the aforementioned process and system 10, provides a novel enhancement in the current strategies to extract active ingredients from plant materials 52.

公开(公告)号：US20210346866A1

公开(公告)日：2021-11-11

申请号：US17376567

申请日：2021-07-15

申请人： Puridify Ltd.

发明人： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Law

IPC分类号： B01J20/26 ,  B01D15/20 ,  B01D15/38 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07K1/16 ,  B01J20/286

摘要： Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.

公开(公告)号：US11124539B2

公开(公告)日：2021-09-21

申请号：US16348534

申请日：2017-11-14

申请人： Cytiva BioProcess R&D AB

发明人： Johan William Ohman ,  Mats Ander ,  Therese Graner ,  Anneli Jorsback ,  Jinyu Zou

IPC分类号： C07K1/22 ,  B01J20/32 ,  B01D15/38 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286

摘要： The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.

公开(公告)号：US11084851B2

公开(公告)日：2021-08-10

申请号：US16113403

申请日：2018-08-27

申请人： EMD Millipore Corporation

发明人： Nanying Bian ,  Neil Soice ,  Shari Spector ,  Kara Levine

IPC分类号： C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32

摘要： The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.

公开(公告)号：US20210016266A1

公开(公告)日：2021-01-21

申请号：US17022304

申请日：2020-09-16

申请人： THE BOARD OF REGENTS, UNIVERSITY OF TEXAS SYSTEM ,  DIONEX CORPORATION

发明人： Purnendu K. DASGUPTA ,  Weixiong HUANG ,  Christopher A. POHL

IPC分类号： B01J39/26 ,  G01N30/60 ,  B01J20/32 ,  B01J20/286 ,  B01D15/36 ,  B01D15/20 ,  B01D15/22 ,  B01J41/20 ,  B01J47/02

摘要： Open tubular capillary columns for liquid and ion chromatography, based upon an ionically impermeable polyolefin capillary having a bore with a sulfonate-group- or amine-group-functionalized internal surface. The capillary columns may include a coating of ion exchanging nanoparticles electrostatically bound to the functionalized internal surface. The capillary columns may be made by exposing the interior surface to a sulfonating reagent comprising chlorosulfonic acid (CISO3H), preferably from 85 wt % to 95 wt % chlorosulfonic acid at a process temperature of 20 to 25° C. The interior surface may be subsequently exposed to an asymmetrical diamine to form a sulfonic mid-linkage to the diamine, i.e., to form a sulfonamide-linked, amine-group-functionalized internal surface. The coating may be provided by subsequently exposing the interior surface to an aqueous suspension of ion exchanging nanoparticles to electrostatically bond the ion exchanging nanoparticles to the functionalized internal surface.