Abstract:
The invention provides a cassette (100) for conducting reactions therein comprising one or more fluid exchange channels (141), a supply chamber (150), a reaction chamber (160) and a waste chamber (170).
Abstract:
An element (1) and method for easily performing liquid assays are disclosed. The element (1) uses capillary action to draw a predetermined volume of a liquid sample into a reaction chamber (66) charged with reagent, where reaction between the liquid sample and the reagent is monitored.
Abstract:
Fluidic multiwell bioreactors are provided as a microphysiological platform for in vitro investigation of multi-organ crosstalks with microbiome for an extended period of time of at least weeks and months. The platform has one or more improvements over existing bioreactors, including on-board pumping for pneumatically driven fluid flow, a redesigned spillway for self- leveling from source to sink, a non-contact built-in fluid level sensing device, precise control on fluid flow profile and partitioning, and facile reconfigurations such as daisy chaining and multilayer stacking. The platform supports the culture of multiple organs together with microbiome in a microphysiological, interacted systems, suitable for a wide range of biomedical applications including systemic toxicity studies and physiology- based pharmacokinetic and pharmacodynamic predictions. A process to fabricate the bioreactors is also provided.
Abstract:
The present invention concerns a microfluidic device or chip including at least one inlet for introducing at least one object into the device; an oil inlet for introducing an oil that supports droplet formation into the device or a droplet forming substance inlet for introducing a droplet forming substance into the device; a co-encapsulation area or structure where the at least one object is encapsulated by the droplet; a microfluidic tubing or channel for transporting the at least one object to an entrance of the co-encapsulation area or structure; an oil supporting droplet formation microchannel or droplet forming substance microchannel connected to the microfluidic tubing or channel to place a liquid of the microfluidic tubing or channel in direct contact with the oil that supports droplet formation or the droplet forming substance; and a droplet microchannel or tubing for transporting the droplet.
Abstract:
A microfluidic assay system including a microfluidic cartridge and an associated microfluidic operator system. The microfluidic cartridge comprises a base part, having a first face with a recess and a foil fixed to the base part for covering the recess and providing a microfluidic cartridge foil face. The base part with the recess and the foil forms a flow channel and a sink in fluid communication with each other. The microfluidic cartridge comprises an inlet opening into the flow channel upstream to the reaction section. The operator system includes a piston, a temperature regulating element and an actuator positioned such that the foil face of the microfluidic cartridge can be positioned in contact with the operative system with the reaction section in close proximity to the temperature regulating element while the actuator is associated to the sink section to depress the foil covering the sink section and the piston is associated to the flow channel at an upstream valve section to depress the foil to close off the flow channel upstream to the reaction section.
Abstract:
A device for monitoring fluid media, and methods of manufacture thereof are disclosed. The monitoring device may be implemented as a single monolithic unit made from a certain type of material without a separate packaging, and having a base element comprising at least one fluid port and at least one cavity or fluid flow path fluidly coupled to the at least one fluid port for enabling fluid exchange of fluid media therewith. The device comprises at least one sensing element associated with the cavity or fluid flow path and configured and operable for measuring at least one property or condition of fluid media introduced therein to, and generate measurement data or signals indicative thereof. Electrical contacts disposed on the base element of the device, and electrically coupled to the at least one sensing element are used for establishing electrical connection with the at least one sensing element.
Abstract:
Optical readers and alignment tools for detecting the level of an analyte. Described herein are small, disposable partially-encapsulated sensing chips for detecting an analyte level from a fluid sample (e.g., a blood sample) having an edge of the integrated sensing chip exposed to directly expose a plurality of excitation and a collection waveguides, as well as optical readers and methods of operating them. A fluid sample maybe applied to a sensing surface of the sensing chip in the housing so that an analyte level can be optically detected. Also described are methods of sensing an analyte using these devices and systems including an optical detector.
Abstract:
Die Erfindung betrifft eine Flusszelle, insbesondere zur Analyse oder/und Synthese von Substanzen mit wenigstens einem Speicherbereich (10;19;33), der mit einem Kanal (7-9;20) zum Transport von Fluid aus dem, in den oder/und durch den Speicherbereich verbunden ist, wobei der Kanal einen durch ein Substrat (1) und eine mit dem Substrat verbundene Folie (3) begrenzten Kanalbereich (14) aufweist, in welchem der Kanal verschlossen und an einer Sollbruchstelle unter Auslenkung der Folie aufschließbar ist. Die erfindungsgemäße Flusszelle ist dadurch gekennzeichnet, dass die Folie eine den Kanalbereich bildende Ausnehmung (15) in dem Substrat abdeckt und in der Ausnehmung eine den Kanal verschließende, einstückig mit dem Substrat verbundene Sperrwand (16) angeordnet ist, dass die Sollbruchstelle durch einen aufbrechbaren Verbindungsbereich zwischen der Folie und einem der Folie zugewandten Randabschnitt (17) der Sperrwand gebildet ist und dass für die Flächenausdehnung des Verbindungsbereichs die Abmessungen einer in dem Randabschnitt gebildeten, zu der Folie parallelen Randfläche der Sperrwand maßgebend sind.
Abstract:
A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.