摘要:
Pseudomonas saccharophila G4 forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.
摘要:
We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.
摘要翻译:我们描述了可从具有非麦芽糖苷外切酶活性的亲本多肽衍生的PS4变体多肽,其中PS4变体多肽在选自以下的一个或多个位置包含氨基酸突变:121,161,223,146,157 ,158,198,229,303,306,309,316,353,26,70,205,188,272,339,参考SEQ ID NO:1所示的假单胞菌属的嗜酸芽胞弧菌外切酶序列的位置编号。
摘要:
There is provided an anti-fouling composition comprising (i) a surface coating material; (ii) an enzyme obtained or obtainable from a marine organism; and (iii) (a) a substrate for the enzyme; and/or (b) a precursor enzyme and a precursor substrate, wherein the precursor enzyme and the precursor substrate are selected such that a substrate for the enzyme is generatable by action of the precursor enzyme on the precursor substrate; wherein the enzyme and the substrate are selected such that an anti-foulant compound is generatable by action of the enzyme on the substrate.
摘要:
An anti-microbial protein comprising a peptide having the amino acid sequence: AA1-AA2-AA3-Cys-AA5-AA6-AA7-AA8-AA9-Cys-AA11-AA12-AA13-AA14-Cys-Cys-AA17-AA18-AA19-AA20-AA21-Cys-AA23-AA24-AA25-AA26-AA27-AA28-Cys-AA30. An anti-microbial protein having the sequence depicted in any one of SEQ ID Nos. 1-3. Recombinant DNA encoding such proteins. A vector comprising such DNA which is expressible in plants and which is linked to a plant operable promoter and terminator. Plants transformed with such recombinant DNA; the progeny of such plants which contain the DNA stably incorporated and hereditable in a Mendelian manner, and/or the seeds of such plants or such progeny.
摘要翻译:包含具有氨基酸序列的肽的抗微生物蛋白质:AA1-AA2-AA3-Cys-AA5-AA6-AA7-AA8-AA9-Cys-AA11-AA12-AA13-AA14-Cys-Cys-AA17-AA18- AA19-AA20-AA21-CYS-AA23-AA24-AA25-AA26-AA27-AA28-CYS-AA30。 具有SEQ ID No.3-1中任一项所示序列的抗微生物蛋白质。 编码这种蛋白质的重组DNA。 包含这样的DNA的载体,其在植物中可表达并与植物可操作的启动子和终止子连接。 用这种重组DNA转化的植物; 这些植物的后代含有以孟德尔方式稳定掺入和遗传的DNA,和/或这种植物或其后代的种子。
摘要:
A method of preparing alpha -1,4-glucan lyase enzymes is described. The method comprises isolating the enzymes from a fungally infected algae. The amino acid sequences of the enzymes have been determined. The nucleic acid sequences coding for the enzymes have also been determined.
摘要:
The present invention relates to the use of an amylase and a lipolytic enzyme in combination to improve the stackability of bread, methods of preparing dough and baked products having a combination of such enzymes, as well as bread having particular bread stackability profiles.
摘要:
The present invention relates to the solubilisation of cereal bran, for preparing compositions comprising soluble fractions of cereal bran and the use of these compositions comprising solubilised cereal bran for the preparation of food products, such as bread.
摘要:
We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G)..
摘要:
The present invention relates to the modification of lipids in lipid-containing plant material, such as cereal bran for the generation of functional lipids. The present invention further relates to the preparation of compositions comprising such functional lipids as well as the use of these compositions comprising functional lipids and other functional compounds derived from the action of lipid modifying enzymes for the preparation of composition suitable for the preparation of bio-ethanol as well as food products, such as bread.
摘要:
Pseudomonas saccharophila G4 forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.