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    Process for the depletion or removal of endotoxins
    4.
    发明授权
    Process for the depletion or removal of endotoxins 失效
    内毒素消耗或去除的过程

    公开(公告)号：US07510826B2

    公开(公告)日：2009-03-31

    申请号：US11412130

    申请日：2006-04-27

    Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

    Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

    IPC: C12Q1/00 ,  G01N30/02 ,  G01N31/00 ,  G01N33/48

    CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10 ,  G01N30/461

    Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.

    Abstract translation: 从含有指定用于治疗用途的活性成分的制剂中消耗或除去内毒素的方法，其通过遗传工程和/或生物技术从天然来源获得，其中所述天然来源被裂解，所得的级分任选地离心， 用亲和层析法过滤或处理; 所述级分用盐水溶液和洗涤剂预先加热，用阴离子交换材料处理，然后用另一种盐溶液洗涤，活性成分从阴离子交换剂中洗脱，然后以本身已知的方式进一步纯化。

    Process for the preparation of endotoxin-free or endotoxin-depleted nucleic acids and/or oligonucleotides for gene therapy
    5.
    发明授权
    Process for the preparation of endotoxin-free or endotoxin-depleted nucleic acids and/or oligonucleotides for gene therapy 有权
    用于制备用于基因治疗的无内毒素或缺乏内毒素的核酸和/或寡核苷酸的方法

    公开(公告)号：US06297371B1

    公开(公告)日：2001-10-02

    申请号：US09253702

    申请日：1999-02-22

    Applicant: Metin Colpan ,  Joachim Schorr ,  Peter Moritz

    Inventor： Metin Colpan ,  Joachim Schorr ,  Peter Moritz

    IPC: C07H2100

    CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10 ,  G01N30/461

    Abstract: A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein said nucleic acids and/or oligonucleotides are isolated or purified from an essentially biological source, characterized in that said essentially biological sources are lysed, the fractions obtained are optionally freed or depleted from the remainder of said biological sources by per se known mechanical methods, such as centrifugation, filtration; the fractions thus treated are subsequently treated with affinity chromatographic material or with inorganic chromatographic material for the removal of endotoxins; followed by isolation of said nucleic acids and/or oligonucleotides on an anion exchanger which is designed such that DNA begins to desorb from the anion exchanger only at an ionic strength corresponding to a sodium chloride solution of a concentration higher by at least 100 mM than one corresponding to the ionic strength at which RNA begins to desorb from the anion exchanger material.

    Abstract translation: 用于分离和纯化用于基因治疗的核酸和/或寡核苷酸的方法，其中所述核酸和/或寡核苷酸从基本上生物来源分离或纯化，其特征在于基本上生物来源被裂解，获得的级分为 通过本身已知的机械方法，例如离心，过滤，任选地从所述生物源的剩余部分中释放或消耗;随后用亲和层析材料或用无机色谱材料处理如此处理的级分以除去内毒素; 然后在阴离子交换器上分离所述核酸和/或寡核苷酸，所述阴离子交换器被设计为使得DNA开始从阴离子交换剂解吸离子，其离子强度对应于浓度高于一个相应浓度至少100mM的氯化钠溶液 到RNA开始从阴离子交换材料解吸的离子强度。

    Process and a device for the isolation of cell components such as nucleic acids from natural sources
    6.
    发明授权
    Process and a device for the isolation of cell components such as nucleic acids from natural sources 失效
    方法和用于分离细胞组分如来自天然来源的核酸的装置

    公开(公告)号：US06277648B1

    公开(公告)日：2001-08-21

    申请号：US08253152

    申请日：1994-06-02

    Applicant: Metin Colpan

    Inventor： Metin Colpan

    IPC: B01D3700

    CPC classification number: C12N15/101 ,  B01D39/00 ,  C12N15/1017 ,  Y10T436/25375 ,  Y10T436/255

    Abstract: Described is a method of isolating cell components, such as nucleic acids, from natural sources by filtering a sample of the digested natural sources such as cells or cell fragments. The method is characterized in that the sample is passed through a filter, the pore size of which decreases in the direction of flow of the sample through the filter.

    Abstract translation: 描述了通过过滤消化的天然来源（例如细胞或细胞碎片）的样品从天然来源分离细胞成分（例如核酸）的方法。 该方法的特征在于，样品通过过滤器，其孔径在样品通过过滤器的流动方向上减小。

    Process and device for the isolation of cell components, such as nucleic acids, from natural sources
    7.
    发明授权
    Process and device for the isolation of cell components, such as nucleic acids, from natural sources 失效
    从天然来源分离细胞成分（如核酸）的方法和装置

    公开(公告)号：US06274371B1

    公开(公告)日：2001-08-14

    申请号：US08809072

    申请日：1997-06-19

    Applicant: Metin Colpan

    Inventor： Metin Colpan

    IPC: C12M133

    CPC classification number: C12N15/101 ,  C12N15/87 ,  Y10S435/975

    Abstract: A filtration process for the preparation of nucleic acids from natural sources is disclosed. The sources containing nucleic acids are lysed; the lysate is left to rest for some time; the resulting lysate passes a filter layer. The filter layer is selected from silica gel, aluminum oxide or packed diatomaceous earth, or interwoven or cemented non-wovens of glass fibers and silica gel. Other filter layers include cellulose, paper, compressed paper, and paper non-wovens. The fraction leaving the filter layer is then collected and the nucleic acid is subsequently isolated and purified from the collected fraction. Furthermore, the filter layer has not been modified with anion exchange groups.

    Abstract translation: 公开了一种用于从天然来源制备核酸的过滤方法。 含有核酸的来源被裂解; 裂解物待休息一段时间; 所得裂解物通过过滤层。 过滤层选自硅胶，氧化铝或填充的硅藻土，或玻璃纤维和硅胶的交织或粘合无纺布。 其它过滤层包括纤维素，纸，压缩纸和纸无纺布。 然后收集离开过滤层的级分，随后从收集的级分中分离和纯化核酸。 此外，过滤层未用阴离子交换基团修饰。

    Process for the separation and purification of nucleic acids from biological sources
    8.
    发明授权
    Process for the separation and purification of nucleic acids from biological sources 失效
    从生物来源分离和纯化核酸的方法

    公开(公告)号：US5990301A

    公开(公告)日：1999-11-23

    申请号：US687529

    申请日：1996-10-18

    Applicant: Metin Colpan ,  Joachim Schorr ,  Peter Moritz

    Inventor： Metin Colpan ,  Joachim Schorr ,  Peter Moritz

    IPC: C12N15/10 ,  C12N15/87 ,  C07H21/00 ,  C12N1/08

    CPC classification number: C12N15/87 ,  C12N15/101 ,  Y10S435/81

    Abstract: A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein nucleic acids and/or oligonucleotides are isolated or purified from an essentially biological source, characterized in thatessentially biological sources are lysed, the fractions obtained are optionally freed or depleted from the remainder of biological sources by per se known mechanical methods, such as centrifugation, filtration;the fractions thus treated are subsequently treated with affinity chromatographic material or with inorganic chromatographic material for the removal of endotoxins; followed byisolation of nucleic acids and/or oligonucleotides on an anion exchanger which is designed such that DNA begins to desorb from the anion exchanger only at an ionic strength corresponding to a sodium chloride solution of a concentration higher by at least 100 mM than one corresponding to the ionic strength at which RNA begins to desorb from the anion exchanger material.

    Abstract translation: PCT No.PCT / EP95 / 00389 371日期：1996年10月18日 102（e）日期1996年10月18日PCT提交1995年2月3日PCT公布。 第WO95 / 21177号公报 日期1995年8月10日一种用于分离和纯化用于基因治疗的核酸和/或寡核苷酸的方法，其中从基本上生物来源分离或纯化核酸和/或寡核苷酸，其特征在于基本上生物来源被裂解， 通过本身已知的机械方法，例如离心，过滤，任选地从所述生物来源的其余部分中释放或消耗所得的级分。 随后用亲和层析材料或用无机色谱材料处理如此处理的级分以除去内毒素; 随后在阴离子交换器上分离核酸和/或寡核苷酸，其被设计为使得DNA开始从阴离子交换剂解吸，仅在对应于浓度高于一个浓度的至少100mM的氯化钠溶液的离子强度 到RNA开始从阴离子交换材料解吸的离子强度。

    METHOD AND DEVICE FOR PERFORMING A NUCLEIC ACID PREPARATION AND/OR AMPLIFICATION
    9.
    发明申请
    METHOD AND DEVICE FOR PERFORMING A NUCLEIC ACID PREPARATION AND/OR AMPLIFICATION 审中-公开
    用于进行核酸制备和/或放大的方法和装置

    公开(公告)号：US20100159532A1

    公开(公告)日：2010-06-24

    申请号：US12640798

    申请日：2009-12-17

    Applicant: Metin COLPAN ,  Ralf Himmelreich ,  Thomas Rothmann

    Inventor： Metin COLPAN ,  Ralf Himmelreich ,  Thomas Rothmann

    IPC: C12P19/34 ,  C12M1/40

    CPC classification number: B01L3/502 ,  B01L3/505 ,  B01L3/5082 ,  B01L7/52 ,  B01L2300/0832 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2547/10

    Abstract: The invention relates to a method and a device for performing a nucleic acid preparation and/or amplification.For the implementation of the method, a flexible tube is provided, which is always sealed below and hence at one end during the implementation of the sample preparation and/or amplification. In this tube, a biological sample is prepared and/or amplified. The use of only one tube is very economical. The tube is a disposable item which after a preparation or amplification can with its contents be disposed of environmentally correctly. Since moreover the tube has only to be filled with sample substances and reagents at a given time and manipulated, the method can also be simply and economically performed automatically.

    Abstract translation: 本发明涉及一种用于进行核酸制备和/或扩增的方法和装置。 为了实现该方法，提供了柔性管，其在样品制备和/或扩增的实施期间总是被密封在下方，因此在其一端被密封。 在该管中，制备和/或扩增生物样品。 只使用一根管是非常经济的。 该管是一种一次性物品，其在制备或扩增后可以使其内容物在环境上被正确地处理。 此外，管子在给定的时间只需要填充样品物质和试剂并进行操作，该方法也可以简单和经济地自动执行。

    Process for the separation of double-stranded/single-stranded nucleic acid structures
    10.
    发明授权
    Process for the separation of double-stranded/single-stranded nucleic acid structures 失效
    分离双链/单链核酸结构的方法

    公开(公告)号：US06180778B2

    公开(公告)日：2001-01-30

    申请号：US08687610

    申请日：1996-08-08

    Applicant: Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

    Inventor： Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

    IPC: C07H2100

    CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6806

    Abstract: The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.

    Abstract translation: 本发明涉及通过将所述核酸整体同时吸收到矿物载体上，然后将其分离成双链和单链核酸的单链和单链核酸部分，将核酸混合物色谱分离成双链和单链核酸部分 通过分批洗脱或通过选择性地将液体样品的双链或单链核酸吸收到矿物载体，以及用于执行根据本发明的方法的溶液和试剂盒来代替。

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