公开(公告)号：US11896969B2

公开(公告)日：2024-02-13

申请号：US17012108

申请日：2020-09-04

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Christopher Belisle ,  Xuemei He ,  Hong Chen ,  Yueping Xu ,  Jiali Liao

IPC: B01J41/07 ,  B01J47/02 ,  C07K1/18 ,  C12N9/88 ,  B01J47/014 ,  B01J41/14

CPC classification number: B01J41/14 ,  B01J41/07 ,  B01J47/014 ,  B01J47/02 ,  C07K1/18 ,  C12N9/88 ,  C12Y402/01045 ,  B01J2220/52

Abstract: Chromatography resins having anionic exchange-hydrophobic mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US11590486B2

公开(公告)日：2023-02-28

申请号：US17008212

申请日：2020-08-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jiali Liao ,  Lee Olech ,  Hong Chen ,  Xuemei He ,  Russell Frost

IPC: B01J39/26 ,  C07K16/06 ,  C07K1/16 ,  C07K1/22

Abstract: A method for manufacturing a mixed-mode chromatography medium is provided. The method can include, for example, oxidizing diol groups on diol-functionalized solid particles having pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter and having a diol density of from about 200 to about 300 μmol/mL to aldehyde groups, thereby converting said diol-functionalized solid particles to aldehyde-functionalized solid particles; and coupling amine-functionalized ligands to said aldehyde-functionalized solid particles, said amine-functionalized ligands comprising an amine-substituted hydrophobic group joined to an acid moiety selected from the group consisting of a carboxyl group and a sulfo group.

公开(公告)号：US11666888B2

公开(公告)日：2023-06-06

申请号：US16967429

申请日：2019-02-05

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Christopher Belisle ,  Hong Chen ,  Yueping Xu ,  Jiali Liao ,  Xuemei He

IPC: B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/289 ,  B01J20/32 ,  C07K16/06 ,  B01J20/26

CPC classification number: B01J20/262 ,  B01D15/363 ,  B01J20/289 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3227 ,  B01J20/3248 ,  B01J20/3253 ,  B01J20/3285 ,  C07K16/065 ,  B01D15/327 ,  B01D15/3847

Abstract: Chromatography resins having mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US10792654B2

公开(公告)日：2020-10-06

申请号：US15063235

申请日：2016-03-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jiali Liao ,  Lee Olech ,  Hong Chen ,  Xuemei He ,  Russell Frost

IPC: B01J39/26 ,  C07K16/06 ,  C07K1/16 ,  C07K1/22

Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand comprising benzamidoacetic acid to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between a phenyl ring and the support matrix.

公开(公告)号：US20130102761A1

公开(公告)日：2013-04-25

申请号：US13654574

申请日：2012-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jiali Liao ,  Lee Olech ,  Hong Chen ,  Xuemei He ,  Russell Frost

IPC: C07K1/22 ,  C08F271/02 ,  C07K16/00

CPC classification number: B01J39/26 ,  C07K1/165 ,  C07K1/22 ,  C07K16/065

Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.

Abstract translation: 蛋白质通过将配体与阳离子交换和疏水功能连接到大孔载体基质形成的混合模式色谱系统来纯化，配体和载体基质之间的唯一连接是具有不超过三个 疏水基团和载体基质之间的原子。

公开(公告)号：US12194432B2

公开(公告)日：2025-01-14

申请号：US18307991

申请日：2023-04-27

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Christopher Belisle ,  Hong Chen ,  Yueping Xu ,  Jiali Liao ,  Xuemei He

IPC: B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/289 ,  B01J20/32 ,  C07K16/06

Abstract: Chromatography resins having mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US09309282B2

公开(公告)日：2016-04-12

申请号：US13654574

申请日：2012-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jiali Liao ,  Lee Olech ,  Hong Chen ,  Xuemei He ,  Russell Frost

IPC: C07K1/22 ,  C07K1/16 ,  C07K16/06 ,  B01D15/08

CPC classification number: B01J39/26 ,  C07K1/165 ,  C07K1/22 ,  C07K16/065

Abstract: Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.

Abstract translation: 蛋白质通过将配体与阳离子交换和疏水功能连接到大孔载体基质形成的混合模式色谱系统来纯化，配体和载体基质之间的唯一连接是具有不超过三个 疏水基团和载体基质之间的原子。