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41 条记录 (耗时 0.021 秒)

    Controlling use of oligonucleotide sequences released from arrays
    1.
    发明申请
    Controlling use of oligonucleotide sequences released from arrays 失效
    控制从阵列释放的寡核苷酸序列的使用

    公开(公告)号：US20070037175A1

    公开(公告)日：2007-02-15

    申请号：US11203328

    申请日：2005-08-11

    申请人： Eric Leproust ,  Michael Booth

    发明人： Eric Leproust ,  Michael Booth

    IPC分类号： C12Q1/68

    CPC分类号： C40B50/18 ,  B01J19/0046 ,  B01J2219/00454 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  C40B40/06

    摘要： A method for controlling the use of oligonucleotide sequences released from arrays, comprises synthesizing a chemical array of oligonucleotides on a substrate under conditions for producing an array of cleavable oligonucleotides that are blocked from enzymatic reactions after cleavage. Methods may also include receiving a chemical array of cleavable oligonucleotides on a substrate, and cleaving the oligonucleotides from the array, wherein the oligonucleotides are blocked from enzymatic reactions after cleavage. Arrays, populations of oligonucleotides and kits are also provided to facilitate the methods.

    摘要翻译： 用于控制从阵列释放的寡核苷酸序列的使用的方法包括在产生可切割寡核苷酸阵列的条件下在底物上合成寡核苷酸的化学阵列，所述可切割寡核苷酸阵列在切割后被阻止酶反应。 方法还可以包括在底物上接收可切割寡核苷酸的化学阵列，并从阵列切割寡核苷酸，其中寡核苷酸在切割后被阻止酶反应。 还提供了阵列，寡核苷酸和试剂盒的种群以便于该方法。

    Linkers and Co-Coupling Agents for Optimization of Oligonucleotide Synthesis and Purification on Solid Supports
    2.
    发明申请
    Linkers and Co-Coupling Agents for Optimization of Oligonucleotide Synthesis and Purification on Solid Supports 有权
    用于优化固体支持物上的寡核苷酸合成和纯化的接头和共偶联剂

    公开(公告)号：US20100093974A1

    公开(公告)日：2010-04-15

    申请号：US12493985

    申请日：2009-06-29

    申请人： Xiaolian Gao ,  Hua Zhang ,  Peilin Yu ,  Eric Leproust ,  Jean Philippe Pellois ,  Qin Xiang ,  Xiaochuan Zhou

    发明人： Xiaolian Gao ,  Hua Zhang ,  Peilin Yu ,  Eric Leproust ,  Jean Philippe Pellois ,  Qin Xiang ,  Xiaochuan Zhou

    IPC分类号： C07K9/00 ,  C07H21/00

    CPC分类号： C07H19/06 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07K2/00 ,  C40B40/06 ,  C40B50/18 ,  C40B80/00

    摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.

    摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。

    Quantification of depurination through the use of two cleavage sites
    3.
    发明申请
    Quantification of depurination through the use of two cleavage sites 审中-公开
    通过使用两个切割位点进行定量分析

    公开(公告)号：US20070224601A1

    公开(公告)日：2007-09-27

    申请号：US11388727

    申请日：2006-03-24

    申请人： Heather McCuen ,  Eric Leproust

    发明人： Heather McCuen ,  Eric Leproust

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837

    摘要： The invention is directed an array comprising at least one depurination probe including a depurination sensitive first cleavage site adjacent a first tag region; and a base-cleavable second cleavage site adjacent a second tag region wherein the first tag region and the second tag region are independently quantifiable upon release from the depurination probe; and a method for use thereof to quantify depurination side reactions and overall yield during in situ array synthesis.

    摘要翻译： 本发明涉及一种阵列，其包括至少一个包含与第一标签区相邻的去血清敏感的第一切割位点的去血清探针; 以及与第二标签区相邻的可裂解的第二切割位点，其中第一标签区和第二标记区在从脱血针探针释放时可独立地定量; 以及其用于定量原位阵列合成期间的脱嘌呤副反应和总产率的方法。

    Oligonucleotide arrays comprising background probes
    5.
    发明申请
    Oligonucleotide arrays comprising background probes 审中-公开
    包含背景探针的寡核苷酸阵列

    公开(公告)号：US20060057587A1

    公开(公告)日：2006-03-16

    申请号：US10939650

    申请日：2004-09-13

    申请人： Scott Vacha ,  Eric Leproust

    发明人： Scott Vacha ,  Eric Leproust

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6837 ,  C12Q2545/101 ,  C12Q2525/161

    摘要： The invention relates to chemical arrays comprising a plurality of features comprising biopolymers at known locations on the substrate, wherein at least one feature comprises specific hybridization probes designed or selected to bind to specific target sequences and background probes for evaluating non-specific binding of target. Methods, systems, and kits for making and using the arrays are also described.

    摘要翻译： 本发明涉及包含多个特征的化学阵列，其包含在底物上已知位置处的生物聚合物，其中至少一个特征包括设计或选择以结合特异性靶序列的特异性杂交探针和用于评估靶的非特异性结合的背景探针。 还描述了用于制造和使用阵列的方法，系统和套件。

    In Situ Nucleic Acid Array Synthesis Compositions
    7.
    发明申请
    In Situ Nucleic Acid Array Synthesis Compositions 审中-公开
    原位核酸阵列合成组合物

    公开(公告)号：US20090325821A1

    公开(公告)日：2009-12-31

    申请号：US12340796

    申请日：2008-12-22

    申请人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    发明人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    IPC分类号： C40B50/18 ,  C40B60/14

    CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00527

    摘要： In situ nucleic acid synthesis compositions are provided. The compositions include a solvent; and at least one of: a viscosity modifier; and a surface tension modifier. During use, the compositions further include one of a phosphoramidite and an activator. Also provide are methods of using the compositions, e.g., in the synthesis of nucleic acid arrays, and systems kits for practicing the methods.

    摘要翻译： 提供原位核酸合成组合物。 组合物包括溶剂; 和至少一种：粘度调节剂; 和表面张力调节剂。 在使用期间，组合物还包括亚磷酰胺和活化剂之一。 还提供使用组合物的方法，例如在核酸阵列的合成中以及用于实施该方法的系统试剂盒。

    In situ nucleic acid array synthesis compositions
    8.
    发明申请
    In situ nucleic acid array synthesis compositions 审中-公开
    原位核酸阵列合成组合物

    公开(公告)号：US20080085513A1

    公开(公告)日：2008-04-10

    申请号：US11545931

    申请日：2006-10-10

    申请人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    发明人： Eric Leproust ,  Bill J. Peck ,  Xiaohua C. Huang

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00527

    摘要： In situ nucleic acid synthesis compositions are provided. The compositions include a solvent; and at least one of: a viscosity modifier; and a surface tension modifier. During use, the compositions further include one of a phosphoramidite and an activator. Also provide are methods of using the compositions, e.g., in the synthesis of nucleic acid arrays, and systems kits for practicing the methods.

    摘要翻译： 提供原位核酸合成组合物。 组合物包括溶剂; 和至少一种：粘度调节剂; 和表面张力调节剂。 在使用期间，组合物还包括亚磷酰胺和活化剂之一。 还提供使用组合物的方法，例如在核酸阵列的合成中以及用于实施该方法的系统试剂盒。

    Chemical arrays and methods of using the same
    10.
    发明申请
    Chemical arrays and methods of using the same 有权

    公开(公告)号：US20060121543A1

    公开(公告)日：2006-06-08

    申请号：US11008384

    申请日：2004-12-08

    申请人： Mel Kronick ,  Eric Leproust

    发明人： Mel Kronick ,  Eric Leproust

    IPC分类号： G01N33/543 ,  G01N33/537

    CPC分类号： C40B30/04 ,  G01N33/54366

    摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.