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    Highly alkaline proteases
    4.
    发明授权
    Highly alkaline proteases 失效
    高碱性蛋白酶

    公开(公告)号：US5352603A

    公开(公告)日：1994-10-04

    申请号：US661378

    申请日：1991-02-28

    申请人： Roman Vetter ,  Detlef Wilke ,  Antoine Amory ,  Andre Clippe ,  Dietmar Schomburg ,  Wolfgang Aehle

    发明人： Roman Vetter ,  Detlef Wilke ,  Antoine Amory ,  Andre Clippe ,  Dietmar Schomburg ,  Wolfgang Aehle

    IPC分类号： C12N1/21 ,  C12N9/54 ,  C12N15/57 ,  C11D3/386 ,  C11D7/42 ,  C12N9/52

    CPC分类号： C12N9/54

    摘要： Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

    摘要翻译： 通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。 突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得，通过定点诱变（点突变）以定点诱变（点突变）改变这些DNA序列，使得点突变位于的密码子现在 编码比原始氨基酸更强碱性的氨基酸。 结果是高碱性蛋白酶，其中原始氨基酸已被更强碱性氨基酸替代，优选由氨基酸赖氨酸或精氨酸代替。 还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸，DNA序列，载体和转化微生物。

    Polymerase chimeras
    5.
    发明授权
    Polymerase chimeras 有权
    聚合酶嵌合体

    公开(公告)号：US06607883B1

    公开(公告)日：2003-08-19

    申请号：US09623326

    申请日：2001-02-08

    申请人： Bruno Frey ,  Britta Villbrandt ,  Dietmar Schomburg ,  Harald Sobek ,  Waltraud Ankenbauer

    发明人： Bruno Frey ,  Britta Villbrandt ,  Dietmar Schomburg ,  Harald Sobek ,  Waltraud Ankenbauer

    IPC分类号： C12Q168

    CPC分类号： C12N9/1252 ,  C07K2319/00

    摘要： The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.

    摘要翻译： 本发明涉及聚合酶嵌合体，其由表示结构域的氨基酸片段组成，并且其结合对于特定应用有利的天然存在的聚合酶的性质。 令人惊讶的是，各种酶的结构域在嵌合体中是活性的，并表现出合作行为。 此外，本发明涉及根据本发明的嵌合体的生产方法和这些嵌合体用于合成核酸的用途，例如， 在聚合酶链反应期间。 此外，本发明涉及包含根据本发明的聚合酶嵌合体的试剂盒。

    Polymerase chimeras
    6.
    发明授权
    Polymerase chimeras 有权
    聚合酶嵌合体

    公开(公告)号：US07244602B2

    公开(公告)日：2007-07-17

    申请号：US10456129

    申请日：2003-06-06

    申请人： Bruno Frey ,  Britta Villbrandt ,  Dietmar Schomburg ,  Harald Sobek ,  Waltraud Ankenbauer

    发明人： Bruno Frey ,  Britta Villbrandt ,  Dietmar Schomburg ,  Harald Sobek ,  Waltraud Ankenbauer

    IPC分类号： C12N9/12 ,  C12Q1/68

    CPC分类号： C12N9/1252 ,  C07K2319/00

    摘要： The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.

    摘要翻译： 本发明涉及聚合酶嵌合体，其由表示结构域的氨基酸片段组成，并且其结合对于特定应用有利的天然存在的聚合酶的性质。 令人惊讶的是，各种酶的结构域在嵌合体中是活性的，并表现出合作行为。 此外，本发明涉及根据本发明的嵌合体的生产方法和这些嵌合体用于合成核酸的用途，例如， 在聚合酶链反应期间。 此外，本发明涉及包含根据本发明的聚合酶嵌合体的试剂盒。