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公开(公告)号：US06607883B1
公开(公告)日：2003-08-19
申请号：US09623326
申请日：2001-02-08
IPC分类号： C12Q168
CPC分类号： C12N9/1252 , C07K2319/00
摘要： The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.
摘要翻译： 本发明涉及聚合酶嵌合体，其由表示结构域的氨基酸片段组成，并且其结合对于特定应用有利的天然存在的聚合酶的性质。 令人惊讶的是，各种酶的结构域在嵌合体中是活性的，并表现出合作行为。 此外，本发明涉及根据本发明的嵌合体的生产方法和这些嵌合体用于合成核酸的用途，例如， 在聚合酶链反应期间。 此外，本发明涉及包含根据本发明的聚合酶嵌合体的试剂盒。
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公开(公告)号：US07244602B2
公开(公告)日：2007-07-17
申请号：US10456129
申请日：2003-06-06
CPC分类号： C12N9/1252 , C07K2319/00
摘要： The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.
摘要翻译： 本发明涉及聚合酶嵌合体，其由表示结构域的氨基酸片段组成，并且其结合对于特定应用有利的天然存在的聚合酶的性质。 令人惊讶的是，各种酶的结构域在嵌合体中是活性的，并表现出合作行为。 此外，本发明涉及根据本发明的嵌合体的生产方法和这些嵌合体用于合成核酸的用途，例如， 在聚合酶链反应期间。 此外，本发明涉及包含根据本发明的聚合酶嵌合体的试剂盒。
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公开(公告)号：US5453372A
公开(公告)日：1995-09-26
申请号：US918318
申请日：1992-07-27
申请人： Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
发明人： Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
CPC分类号： C11D3/38663 , C11D3/386 , C12N9/00 , C12N9/20 , C12N9/54
摘要： Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.
摘要翻译： 针对离子表面活性剂的不稳定作用而进行的酶稳定化，以及生产这样的稳定化酶的方法，其中编码酶的DNA序列通过与定义的酶的表面区域相关的限定位置的定向突变来修饰， 突变位于的密码子现在编码与原始氨基酸不同的氨基酸。
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公开(公告)号：US20100003709A1
公开(公告)日：2010-01-07
申请号：US11793367
申请日：2005-12-13
申请人： Peter Reinemer , Heike Gielen-Haertwig , Ulrich Rosentreter , Volkhart Li , Axel Harrenga , Dietmar Schomburg , Karsten Niefind , Guido Hansen
发明人： Peter Reinemer , Heike Gielen-Haertwig , Ulrich Rosentreter , Volkhart Li , Axel Harrenga , Dietmar Schomburg , Karsten Niefind , Guido Hansen
IPC分类号： C12Q1/37 , C12N9/66 , G06G7/48 , C07D239/22
CPC分类号： C07D239/36 , C07K2299/00 , C12N9/6448 , C12Y304/21037
摘要： This invention relates to crystallised human neutrophil elastase and the use of its three-dimensional structure to design modulators for human neutrophil elastase.
摘要翻译： 本发明涉及结晶人嗜中性粒细胞弹性蛋白酶及其三维结构用于设计人嗜中性粒细胞弹性蛋白酶调节剂的用途。
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公开(公告)号：US5352603A
公开(公告)日：1994-10-04
申请号：US661378
申请日：1991-02-28
申请人： Roman Vetter , Detlef Wilke , Antoine Amory , Andre Clippe , Dietmar Schomburg , Wolfgang Aehle
发明人： Roman Vetter , Detlef Wilke , Antoine Amory , Andre Clippe , Dietmar Schomburg , Wolfgang Aehle
CPC分类号： C12N9/54
摘要： Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.
摘要翻译： 通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。 突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得，通过定点诱变（点突变）以定点诱变（点突变）改变这些DNA序列，使得点突变位于的密码子现在 编码比原始氨基酸更强碱性的氨基酸。 结果是高碱性蛋白酶，其中原始氨基酸已被更强碱性氨基酸替代，优选由氨基酸赖氨酸或精氨酸代替。 还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸，DNA序列，载体和转化微生物。
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公开(公告)号：US4987072A
公开(公告)日：1991-01-22
申请号：US907446
申请日：1986-09-11
申请人： Hans Reichenbach , Gerhard Hofle , Hermann Augustiniak , Norbert Bedorf , Klaus Gerth , Herbert Irschik , Rolf Jansen , Brigitte Kunze , Dietmar Schomburg , Heinrich Steinmetz , Wolfram Trowitzsch-Kienast , Victor Wray
发明人： Hans Reichenbach , Gerhard Hofle , Hermann Augustiniak , Norbert Bedorf , Klaus Gerth , Herbert Irschik , Rolf Jansen , Brigitte Kunze , Dietmar Schomburg , Heinrich Steinmetz , Wolfram Trowitzsch-Kienast , Victor Wray
IPC分类号： C07D323/00 , A61K31/357 , A61K31/365 , A61P31/04 , A61P31/12 , C07D493/22 , C07H17/08 , C12P17/18 , C12P19/62 , C12R1/01
CPC分类号： C07D493/22 , C07H17/08 , C12P19/623
摘要： The invention relates to cyclic antibiotics of formula ##STR1## wherein the prefix R represents that the substituents on the adjacent carbon atom are in the R configuration; the prefix S represents that the substituents on the adjacent carbon atom are in the S configuration; R.sub.1 is hydrogen or hydroxy and R.sub.2 is hydroxy or .beta.-glucopyranosyloxy, and to the hydrates and salts thereof, especially pharmaceutically acceptable salts.The compounds of formula I are prepared by fermentation using the strain So ce 12 (NCIB 12134) of the species Sorangium celulosum and are effective antiobiotics.
摘要翻译： 本发明涉及式（I）的环状抗生素，其中前缀R表示相邻碳原子上的取代基为R构型; 前缀S表示相邻碳原子上的取代基为S构型; R1是氢或羟基，R2是羟基或β-吡喃葡萄糖基氧基，以及其水合物和盐，特别是药学上可接受的盐。 式Ⅰ化合物是通过使用种类Sorangium celulosum的菌株So ce 12（NCIB 12134）进行发酵而制备的，并且是有效的抗生素。
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公开(公告)号：US5096922A
公开(公告)日：1992-03-17
申请号：US512963
申请日：1990-04-23
申请人： Hans Reichenbach , Gerhard Hofle , Hermann Augustiniak , Norbert Bedorf , Klaus Gerth , Herbert Irschik , Rolf Jansen , Brigitte Kunze , Dietmar Schomburg , Heinrich Steinmetz , Wolfram Trowitzsch-Kienast , Victor Wray
发明人： Hans Reichenbach , Gerhard Hofle , Hermann Augustiniak , Norbert Bedorf , Klaus Gerth , Herbert Irschik , Rolf Jansen , Brigitte Kunze , Dietmar Schomburg , Heinrich Steinmetz , Wolfram Trowitzsch-Kienast , Victor Wray
IPC分类号： C07D323/00 , A61K31/357 , A61K31/365 , A61P31/04 , A61P31/12 , C07D493/22 , C07H17/08 , C12P17/18 , C12P19/62 , C12R1/01
CPC分类号： C07D493/22 , C07H17/08 , C12P19/623
摘要： The invention relates to cyclic antibiotics of formula ##STR1## wherein the prefix R represents that the substituents on the adjacent carbon atom are in the R configuration; the prefix S represents that the substituents on the adjacent carbon atom are in the S configuration;R.sub.1 is hydrogen or hydroxy and R.sub.2 is hydroxy and salts thereof, especially pharmaceutically acceptable salts.The compounds of formula I are prepared by fermentation using the strain So ce 12 (NCIB 12134) of the species Sorangium cellulosum and are effective antibiotics.
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