摘要:
A DNA sequence for the gene therapy of tumors is described. In its essential elements, the DNA sequence is composed of an activator sequence, a promoter module and a gene for the active substance. The activator sequence is activated, in a cell-specific manner, in proliferating endothelial cells or in cells which are adjacent to these endothelial cells. This activation is regulated by the promoter module in a cell cycle-specific manner. The active substance is an inhibitor of angiogenesis or a cytostatic or cytotoxic molecule. The DNA sequence is inserted into a viral or non-viral vector which is supplemented with a ligand which possesses affinity for the activated endothelial cell.
摘要:
The invention relates to a method of using a pair of leucine zipper peptides for in vitro diagnosis, in particular, for the immunochemical detection and determination of an analyte in a biological liquid. In one method, the first leucine zipper peptide is immobilized by attaching it to a solid support, the second leucine zipper peptide is coupled to a specific binding partner for the analyte, the two peptides are brought into contact, the sample of the biological liquid is brought into contact with the immobilized first peptide and the specific binding partner for the analyte, and the amount of analyte bound to the binding partner is determined. The leucine zipper peptides are preferably v-fos and c-jun.
摘要:
The invention relates to promoter sequences for a gene comprising a tissue inhibitor of metalloproteinase-3 (TIMP-3). This inhibitor is found in particular in macrophages, synovial cells, and connective tissue cells. The invention also relates to cell-specific gene therapy of a subject, wherein expression of a gene in a tissue is regulated by the aforementioned promoter sequence operationally coupled to said gene. The promoter may also be used in diagnostic methods.
摘要:
Target cell-specific, non-viral vectors for inserting genes into cells, pharmaceuticals composition comprising such vectors, and methods of their use. Target cell-specific, non-viral vectors for inserting at least one gene into cells of an organism, comprising a complex comprising the following components:a) a non-viral carrier for the gene to be inserted,b) a ligand which can bind specifically to the desired target cell,c) a fusion protein for the penetration of the vector into the cytoplasm of the target cell, andd) the gene to be introducedare disclosed. Vectors of this nature are used, for example, in gene therapy.
摘要:
The production of polysaccharide networks, especially starch networks, having a high network density, high solidity, a low swelling degree, and exhibiting reduced water absorption and to the uses thereof, especially, in the filed of biodegradable plastics.
摘要:
Compositions and methods are disclosed for substantially liquid, gel, suspension, slurry, semisolid and/or colloid storage of biological samples following admixture with the herein disclosed storage composition, permitting substantial recovery of biological activity following storage without refrigeration. In certain embodiments, unfractionated blood or tissue samples may be stored without refrigeration for weeks, months or years in a form that permits recovery of intact DNA, RNA or protein components following the storage period.
摘要:
A printing press, in particular a sheet-fed offset printing press, includes at least one control device, a plurality of printing units, one or more varnishing units, and one or more dryers. Variables determining a dryness of the printing material are determined and used to optimize the drying process. For that purpose, the important material streams influencing the drying process are determined at least for the region of the printing press that contains the drying device(s).
摘要:
Polyvinyl alcohol gels, particularly hydrogels, which are produced by methods and formulas and are provided with a characteristic profile that is adjustable to a large extent, e.g., outstanding mechanical properties with moduli of elasticity of 30 MPa and stiffnesses of 25 MPa at a breaking elongation of 550 percent, or full transparency in a wide range of water contents.
摘要:
The present invention relates to nucleic acid sequences and proteins derivable therefrom that have been identified in Sorangium cellulosum, which proteins are catalytically active or participate in the biosynthetic pathway of disorazoles. The invention provides novel sequences which are necessary components of the disorazole biosynthetic pathway in addition to genes dszA-D.
摘要:
Polymer mixtures containing a first long-chain polymer and a second short-chain polymer. The structural parameters of the two polymers are coordinated in such a way that a polymeric material is obtained which is easier to process while being provided with improved mechanical properties and in part also improved thermal properties compared with the first polymer at suitable conditions for producing the polymer mixture as a result of networks being formed and heterocrystallization taking place.