公开(公告)号：US10975433B2

公开(公告)日：2021-04-13

申请号：US16017367

申请日：2018-06-25

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko Ito ,  Mika Inada ,  Koji Hashimoto

IPC: C12Q1/6876 ,  C12Q1/6848 ,  C12P19/34 ,  C12N15/10

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

公开(公告)号：US10557165B2

公开(公告)日：2020-02-11

申请号：US15691337

申请日：2017-08-30

Applicant: Kabushiki Kaisha Toshiba

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/68 ,  C12Q1/6837 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6851 ,  C12N15/09

Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

公开(公告)号：US20190285567A1

公开(公告)日：2019-09-19

申请号：US16115870

申请日：2018-08-29

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: G01N27/327 ,  B01L3/00 ,  C12Q1/6844

Abstract: According to one embodiment, a nucleic acid reaction tool includes a support having a first surface, a covering body having a second surface, and a groove opened on the second surface, and a primer set. The covering body is in contact with the support to form a reaction space surrounded by the first surface and the groove. The groove includes, on an inner surface of the reaction space, rising surfaces opposed to each other, and a rear surface connecting one end of the side surfaces, and a primer fixing region to which the primer set is fixed, the primer fixing region being located at a corner where the one end of the side surfaces connected to the rear surface in the reaction space.

公开(公告)号：US20180274022A1

公开(公告)日：2018-09-27

申请号：US15908044

申请日：2018-02-28

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Mika INADA ,  Koji Hashimoto ,  Keiko Ito

IPC: C12Q1/6853

CPC classification number: C12Q1/6853 ,  C12Q2521/107 ,  C12Q2525/101 ,  C12Q2525/207 ,  C12Q2527/101

Abstract: In general, according to one embodiment, a method containing, reverse-transcribing the first sequence in the target RNA, to obtain a reverse-transcription product containing a first′ sequence complementary to the first sequence, dissociating the reverse-transcription product from the target RNA, hybridizing an elongation primer and the reverse-transcription product to elongate both, thereby obtain an elongation product, and maintaining the amplification reaction liquid containing the elongation product, a primer set and Tin(exo-) DNA polymerase and/or Bsm DNA polymerase under an amplification reaction condition, to amplify the first′ sequence.

公开(公告)号：US20140148359A1

公开(公告)日：2014-05-29

申请号：US14173442

申请日：2014-02-05

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Masayoshi TAKAHASHI ,  Koji Hashimoto ,  Masaru Nikaido ,  Nobuhiro Gemma ,  Jun Okada ,  Daiji Hirosawa ,  Keiichi Yamamoto ,  Tetsuya Kuwabara ,  Madoka Takase ,  Akiko Shimojo ,  Akiyoshi Nakamura

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  B01J2219/00452 ,  B01J2219/00497 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L7/525 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/163

Abstract: According to one embodiment, a multi-nucleic-acid amplification reaction tool includes a support and a plurality of types of primer sets. The support is configured to be able to support a reaction field of a liquid phase. A plurality of types of primer sets are fixed in a releasable state, for each type, on mutually independent fixing regions of at least a surface of the support, which is in contact with the reaction field, when the liquid phase forms the reaction field. A plurality of types of primer sets are configured to amplify the respectively corresponding target sequences.

Abstract translation: 根据一个实施方案，多核酸扩增反应工具包括载体和多种类型的引物组。 支撑体构造成能够支撑液相的反应场。 当液相形成反应场时，多种类型的底漆组固定在每种类型的可释放状态下，在与反应场接触的至少一个表面的相互独立的固定区上。 多种类型的引物组被配置为放大相应的目标序列。

公开(公告)号：US11353418B2

公开(公告)日：2022-06-07

申请号：US16115870

申请日：2018-08-29

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: G01N27/327 ,  B01L3/00 ,  C12Q1/6844 ,  B01L7/00

Abstract: According to one embodiment, a nucleic acid reaction tool includes a support having a first surface, a covering body having a second surface, and a groove opened on the second surface, and a primer set. The covering body is in contact with the support to form a reaction space surrounded by the first surface and the groove. The groove includes, on an inner surface of the reaction space, rising surfaces opposed to each other, and a rear surface connecting one end of the side surfaces, and a primer fixing region to which the primer set is fixed, the primer fixing region being located at a corner where the one end of the side surfaces connected to the rear surface in the reaction space.

公开(公告)号：US20190127793A1

公开(公告)日：2019-05-02

申请号：US16017367

申请日：2018-06-25

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko ITO ,  Mika Inada ,  Koji Hashimoto

IPC: C12Q1/6876 ,  C12N15/10 ,  C12P19/34

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

公开(公告)号：US11952620B2

公开(公告)日：2024-04-09

申请号：US17095139

申请日：2020-11-11

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6851 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/00 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/18 ,  C12Q1/6851 ,  C12Q2527/101 ,  C12Q2531/101 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.

公开(公告)号：US11130988B2

公开(公告)日：2021-09-28

申请号：US15919947

申请日：2018-03-13

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko Ito ,  Koji Hashimoto

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6806

Abstract: According to one embodiment, a detection method is a method for detecting a plurality of target nucleic acids in a sample. The method includes (a) preparing a chain-elongation nucleic acid set group, a primer set, and a probe immobilized substrate, (b) obtaining the target nucleic acid and a long-chain nucleic acid group containing a first sub-chain-elongation nucleic acid and a second sub-chain-elongation nucleic acid, (c) obtaining an amplification product group by maintaining the long-chain nucleic acid group and the primer set under amplification conditions, (d) detecting presence/absence and/or an amount of hybridization, and (e) detecting the plurality of target nucleic acids.

公开(公告)号：US20180282792A1

公开(公告)日：2018-10-04

申请号：US15919947

申请日：2018-03-13

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko ITO ,  Koji Hashimoto

IPC: C12Q1/6816 ,  C12Q1/6806

Abstract: According to one embodiment, a detection method is a method for detecting a plurality of target nucleic acids in a sample. The method includes (a) preparing a chain-elongation nucleic acid set group, a primer set, and a probe immobilized substrate, (b) obtaining the target nucleic acid and a long-chain nucleic acid group containing a first sub-chain-elongation nucleic acid and a second sub-chain-elongation nucleic acid, (c) obtaining an amplification product group by maintaining the long-chain nucleic acid group and the primer set under amplification conditions, (d) detecting presence/absence and/or an amount of hybridization, and (e) detecting the plurality of target nucleic acids.