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公开(公告)号：US20110281768A1
公开(公告)日：2011-11-17
申请号：US13019220
申请日：2011-02-01
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
摘要翻译： 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US20090298075A1
公开(公告)日：2009-12-03
申请号：US12413258
申请日：2009-03-27
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
IPC分类号： C12Q1/68
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US20120289408A1
公开(公告)日：2012-11-15
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08153375B2
公开(公告)日：2012-04-10
申请号：US12413258
申请日：2009-03-27
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08455193B2
公开(公告)日：2013-06-04
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08309330B2
公开(公告)日：2012-11-13
申请号：US13019220
申请日：2011-02-01
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
摘要翻译： 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US08802600B2
公开(公告)日：2014-08-12
申请号：US11731748
申请日：2007-03-29
申请人： David R. Rank , Jeffery Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto , Ron Cicero
发明人： David R. Rank , Jeffery Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto , Ron Cicero
IPC分类号： C40B99/00
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00427 , B01J2219/0045 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y20/00 , B82Y30/00 , C12Q1/6837 , Y10T29/49002 , C12Q2565/629 , C12Q2527/127
摘要： Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
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8.发明授权Articles having localized molecules disposed thereon and methods of producing same 有权具有位于其上的局部分子的制品及其制备方法公开(公告)号：US08772202B2
公开(公告)日：2014-07-08
申请号：US13487978
申请日：2012-06-04
申请人： David R. Rank , Jeffrey Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto , Ronald L. Cicero
发明人： David R. Rank , Jeffrey Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto , Ronald L. Cicero
IPC分类号： C40B99/00
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00427 , B01J2219/0045 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y20/00 , B82Y30/00 , C12Q1/6837 , Y10T29/49002 , C12Q2565/629 , C12Q2527/127
摘要： Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
摘要翻译： 通过使用底物的元素来辅助活性化学基团在基底的所需区域中的定位来制备具有选定的活性化学区域的基底的方法。 所述方法可以包括用于沉积，去除，激活和/或去活衬底的选定区域中的化学基团的光学，化学和/或机械方法，以提供衬底的选择性活性区域。
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公开(公告)号：US20080176769A1
公开(公告)日：2008-07-24
申请号：US11978171
申请日：2007-10-26
申请人： David R. Rank , Jeffery Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto
发明人： David R. Rank , Jeffery Wegener , Jonas Korlach , Daniel Roitman , Yue Xu , John Lyle , Stephen Turner , Paul Peluso , Geoff Otto
IPC分类号： C40B80/00
CPC分类号： C12Q1/6874 , B01J19/0046 , B01J2219/00427 , B01J2219/0045 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y20/00 , B82Y30/00 , C12Q1/6837 , Y10T29/49002 , C12Q2565/629 , C12Q2527/127
摘要： Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
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10.发明申请Protein engineering strategies to optimize activity of surface attached proteins 审中-公开蛋白质工程策略来优化表面附着蛋白质的活性公开(公告)号：US20100260465A1
公开(公告)日：2010-10-14
申请号：US11645135
申请日：2006-12-21
申请人： David Hanzel , Jonas Korlach , Paul Peluso , Geoff Otto , Thang Pham , David Rank , Stephen Turner
发明人： David Hanzel , Jonas Korlach , Paul Peluso , Geoff Otto , Thang Pham , David Rank , Stephen Turner
IPC分类号： G02B6/00 , C12N9/00 , C12N9/10 , C12N9/48 , C12N9/16 , C12N9/02 , C12N9/84 , C12N9/38 , C07K14/00 , B32B1/00 , B32B17/06 , B32B18/00 , B32B9/00
CPC分类号： C12N9/22 , C07K2319/21 , Y10T428/2991 , Y10T428/2993 , Y10T428/2996 , Y10T428/31663 , Y10T428/31768
摘要： Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.
摘要翻译： 提供了包括表面结合结构域，与其结合的活性酶的表面和将酶与表面偶联的方法的分离和/或重组酶。 酶可以包括用于表面偶联的大的和/或多个表面耦合域。
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