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    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS
    3.
    发明申请
    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS 有权

    公开(公告)号：US20170145500A1

    公开(公告)日：2017-05-25

    申请号：US15423744

    申请日：2017-02-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS
    4.
    发明申请
    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS 有权
    双向顺序组合物和方法

    公开(公告)号：US20150353994A1

    公开(公告)日：2015-12-10

    申请号：US14832772

    申请日：2015-08-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    Abstract translation: 在一些实施方案中，从与支持物连接的核酸模板获得序列信息的方法包括将第一引物与连接于载体的模板链杂交，对核酸模板的一部分进行测序，从而形成延伸的第一引物产物， 与一部分核酸模板互补。在一些实施方案中，该方法还包括将切口引入与延伸的第一引物产物杂交的模板链的一部分中，使用下述方法从模板中降解模板链的一部分： 其中一部分延伸的第一引物保留与模板链的未降解部分杂交，并通过合成测序延伸的第一引物的至少一些单链部分。

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    5.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20190338343A1

    公开(公告)日：2019-11-07

    申请号：US16514814

    申请日：2019-07-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/6806 ,  C12Q1/6844

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS
    6.
    发明申请
    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS 审中-公开

    公开(公告)号：US20180030534A1

    公开(公告)日：2018-02-01

    申请号：US15730536

    申请日：2017-10-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    7.
    发明公开
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20240102079A1

    公开(公告)日：2024-03-28

    申请号：US18241617

    申请日：2023-09-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/6806 ,  C12Q1/6844

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    MULTIPLEX NUCLEIC ACID AMPLIFICATION
    9.
    发明申请
    MULTIPLEX NUCLEIC ACID AMPLIFICATION 审中-公开
    多功能核酸扩增

    公开(公告)号：US20150361481A1

    公开(公告)日：2015-12-17

    申请号：US14736509

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David JOUN ,  Chieh-Yuan LI ,  Brian REED ,  Craig OBERGFELL ,  Devin DRESSMAN ,  Abraham ROSENBAUM ,  Scott BENSON ,  Andi BROKA ,  Srinka GHOSH

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2525/191

    Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

    Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散载体的单个反应混合物和多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 靶核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和第二靶核酸群，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合到第二群珠中的珠粒。 单个反应混合物可以用于单层扩增第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

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