公开(公告)号：US20150090936A1

公开(公告)日：2015-04-02

申请号：US14566166

申请日：2014-12-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Hee Chol KANG

IPC: C09K11/06 ,  C07D209/26 ,  C07H19/207 ,  C07D401/10

CPC classification number: C07H19/207 ,  C07D209/14 ,  C07D209/26 ,  C07D401/10 ,  C07D401/14 ,  C09B23/0041 ,  C09B23/0066 ,  C09B23/086 ,  C09B23/164 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1044 ,  C09K2211/1059 ,  C09K2211/1074 ,  G01N33/52

Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

Abstract translation: 本文提供具有大的斯托克斯位移的七甲基氰花青染料及其盐和共轭物。 还提供了使用和制备这种大的斯托克斯位移染料作为荧光共振能量转移（FRET）受体或供体的方法。

公开(公告)号：US20140080966A1

公开(公告)日：2014-03-20

申请号：US14081263

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Nini H. KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang Ying ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C07F7/10 ,  C08K3/20 ,  C08F236/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20170369431A1

公开(公告)日：2017-12-28

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51 ,  C07C2603/50

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20170045496A1

公开(公告)日：2017-02-16

申请号：US15304800

申请日：2015-05-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Hee Chol KANG ,  Wenjun ZHOU ,  Bradley DUBBELS ,  Michael OLSZOWY ,  Michael O'GRADY

IPC: G01N33/50 ,  C09B11/28 ,  C07K16/28

CPC classification number: G01N33/5005 ,  C07C309/61 ,  C07C2603/24 ,  C07D471/04 ,  C07K16/2812 ,  C09B11/28 ,  G01N33/5014 ,  G01N33/5044

Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

Abstract translation: 本文描述的是荧光化合物和方法并且包含这些化合物。 本文公开的化合物是在紫外/可见光谱的红色部分中发荧光的卡吡泼因试剂，并提供明亮的荧光强度，均匀的细胞染色和活细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：US20180078662A1

公开(公告)日：2018-03-22

申请号：US15826593

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian AGNEW ,  Robert AGGELER ,  Hee Chol KANG ,  Aimei CHEN ,  Kyle GEE

IPC: A61K51/10 ,  G01N33/534 ,  C12P21/00 ,  C07K16/18 ,  G01N33/68 ,  C12P21/06 ,  C07K14/435 ,  G01N33/58

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

公开(公告)号：US20170247504A1

公开(公告)日：2017-08-31

申请号：US15491650

申请日：2015-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Xin WANG ,  Hee Chol KANG ,  Aimei CHEN ,  Yexin WU ,  Yi-Zhen HU ,  Robert AGGELER ,  Quentin LOW

IPC: C08G61/12 ,  G01N33/545 ,  G01N33/58 ,  G01N33/533 ,  C07F7/08 ,  C09K11/06

CPC classification number: C08G61/123 ,  C07F7/0816 ,  C07F7/083 ,  C08G2261/122 ,  C08G2261/124 ,  C08G2261/1424 ,  C08G2261/145 ,  C08G2261/1644 ,  C08G2261/1646 ,  C08G2261/3142 ,  C08G2261/3244 ,  C08G2261/334 ,  C08G2261/344 ,  C08G2261/411 ,  C08G2261/522 ,  C08G2261/5222 ,  C08G2261/72 ,  C08G2261/94 ,  C08L65/00 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/1491 ,  G01N33/533 ,  G01N33/545 ,  G01N33/582 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/0094

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US20150284496A1

公开(公告)日：2015-10-08

申请号：US14745815

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang YING ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C08F230/08

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。

公开(公告)号：US20170166603A1

公开(公告)日：2017-06-15

申请号：US15438006

申请日：2017-02-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Hee Chol KANG

IPC: C07H19/207 ,  C09K11/06 ,  C07D209/14 ,  C09B23/16 ,  C07D401/14

CPC classification number: C07H19/207 ,  C07D209/14 ,  C07D209/26 ,  C07D401/10 ,  C07D401/14 ,  C09B23/0041 ,  C09B23/0066 ,  C09B23/086 ,  C09B23/164 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1044 ,  C09K2211/1059 ,  C09K2211/1074 ,  G01N33/52

Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

公开(公告)号：US20160334393A1

公开(公告)日：2016-11-17

申请号：US15221230

申请日：2016-07-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Michael JANES ,  Iain JOHNSON ,  Hee Chol KANG

IPC: G01N33/52 ,  G01N33/58 ,  C07F9/54 ,  C07D491/16 ,  C07K5/02

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

公开(公告)号：US20160326291A1

公开(公告)日：2016-11-10

申请号：US15212532

申请日：2016-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang YING ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C08F230/08

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.