中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

27 records (took 0.028 seconds)

    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES
    1.
    发明申请
    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开

    公开(公告)号：US20200017846A1

    公开(公告)日：2020-01-16

    申请号：US16542121

    申请日：2019-08-15

    Applicant: Life Technologies Corporation

    Inventor： John DAVIDSON ,  Theo NIKIFOROV ,  Guobin LUO

    IPC: C12N11/10 ,  C12N9/12

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    3.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20170260512A1

    公开(公告)日：2017-09-14

    申请号：US15481858

    申请日：2017-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    4.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20200277580A1

    公开(公告)日：2020-09-03

    申请号：US16850629

    申请日：2020-04-16

    Applicant: Life Technologies Corporation

    Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Guobin LUO ,  Mindy LANDES ,  Daniel MAZUR ,  Eileen TOZER ,  Tommie LINCECUM

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权

    公开(公告)号：US20220162660A1

    公开(公告)日：2022-05-26

    申请号：US17667334

    申请日：2022-02-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Hua YU ,  Theo NIKIFOROV ,  Abraham ROSENBAUM

    IPC: C12P19/34 ,  C12Q1/6853 ,  C12Q1/6844

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5′ domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3′ domain, wherein the primer is not extendable by a polymerase, and wherein the 3′ domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    8.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 有权

    公开(公告)号：US20220162573A1

    公开(公告)日：2022-05-26

    申请号：US17645146

    申请日：2021-12-20

    Applicant: Life Technologies Corporation

    Inventor： Peter VANDER HORN ,  Theo NIKIFOROV ,  Guobin LUO ,  Mindy LANDES ,  Daniel MAZUR ,  Eileen TOZER ,  Tommie LINCECUM

    IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME
    9.
    发明申请
    POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 有权

    公开(公告)号：US20210371834A1

    公开(公告)日：2021-12-02

    申请号：US17445047

    申请日：2021-08-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter VANDER HORN ,  Daniel MAZUR ,  Theo NIKIFOROV ,  Mindy LANDES ,  Eileen TOZER

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS
    10.
    发明申请
    BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS 审中-公开

    公开(公告)号：US20180030534A1

    公开(公告)日：2018-02-01

    申请号：US15730536

    申请日：2017-10-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

Patent Agency Ranking