公开(公告)号：US20240216581A1

公开(公告)日：2024-07-04

申请号：US17911188

申请日：2021-09-17

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Xu HAN ,  Hanlian LIU ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Jun WANG

IPC: A61L27/52 ,  A61L27/20 ,  A61L27/22 ,  B33Y10/00 ,  B33Y80/00 ,  C08J3/075

CPC classification number: A61L27/52 ,  A61L27/20 ,  A61L27/222 ,  B33Y10/00 ,  B33Y80/00 ,  C08J3/075 ,  C08J2305/04 ,  C08J2401/28 ,  C08J2405/00 ,  C08J2405/08

Abstract: A photocurable composite hydrogel matrix precursor, a preparation method thereof and a scaffold with same. The photocurable composite hydrogel matrix precursor includes gelatin methacrylate, sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate, where the mass ratio of a photoinitiator to the gelatin methacrylate to the sodium alginate to the sodium carboxymethyl cellulose to the chondroitin sulfate is (0.2-0.3):(8-10):(1-3):(0.6-0.8):(0.05-0.07). By adoption of the precursor, a cell-loaded printing hydrogel scaffold can be obtained through an extrusion-based 3D bio-printing technology, and the scaffold is controllable in form, good in moldability, high in precision, and has good stability; the biocompatibility and bioactivity are high, so that a good growing environment can be provided for fibroblasts; and the preparation process is simple and can be completed within a short time, moreover, the porosity and mechanical performance of the 3D printing hydrogel scaffold can be adjusted by adjusting the raw material ratio.

公开(公告)号：US20220105682A1

公开(公告)日：2022-04-07

申请号：US16479828

申请日：2018-10-17

Applicant: SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhen WANG ,  Jun WANG ,  Bin ZOU ,  Hanlian LIU ,  Hongtao ZHU ,  Peng YAO

IPC: B29C64/35 ,  B08B3/12 ,  B08B3/08 ,  B33Y40/20

Abstract: A self-rotation cleaning device has outer and inner housings, a workpiece rotating system, an ultrasonic cleaning system and a fluid perfusion system. The inner housing is in the outer housing in a horizontal direction. A cylindrical cavity is inside the inner housing. One end of the inner housing has a sealing cover detachably connected thereto, and the other end is closed. The workpiece rotating system is in the cavity for fixing a member to be cleaned, and realizes self-rotation of the member. The ultrasonic cleaning system supplies mechanical energy to the cleaning liquid in the inner housing to generate bubbles therein. The bubbles remove residual resin attached to the cleaned member surface by continuous vibration and burst. The fluid perfusion system provides self-rotation power for the cleaned member, and continuously delivers the cleaning liquid to the inside of the cleaned member, and the cleaning liquid is carried out after cleaning.

公开(公告)号：US20240302352A1

公开(公告)日：2024-09-12

申请号：US18182091

申请日：2023-03-10

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhuang CHEN ,  Hanlian LIU ,  Peng YAO ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Zhen WANG ,  Jun WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU ,  Minting WANG ,  Yabin GUAN

IPC: G01N33/50 ,  B33Y70/00 ,  B33Y80/00 ,  C12N5/079 ,  C12N5/0793 ,  C12N5/0797

CPC classification number: G01N33/5082 ,  B33Y70/00 ,  B33Y80/00 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2501/13 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/74

Abstract: A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.

公开(公告)号：US20240067567A1

公开(公告)日：2024-02-29

申请号：US17955237

申请日：2022-09-28

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zexin LI ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Jun WANG ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU

IPC: C04B35/117 ,  B23B27/14 ,  C04B35/626 ,  C04B35/645

CPC classification number: C04B35/117 ,  B23B27/148 ,  C04B35/6261 ,  C04B35/6264 ,  C04B35/62655 ,  C04B35/645 ,  C04B2235/3206 ,  C04B2235/3217 ,  C04B2235/3224 ,  C04B2235/3258 ,  C04B2235/3856 ,  C04B2235/3886 ,  C04B2235/606 ,  C04B2235/656 ,  C04B2235/6581 ,  C04B2235/9607

Abstract: An alumina-based ceramic tool material with low thermal expansion and a preparation process thereof, accordingly, the ceramic tool material may have both the high hardness of alumina ceramics after the hot pressing sintering, and reduces the thermal expansion coefficient of the overall ceramic material by adding the Sc2W3O12 as the negative thermal expansion phase, which improves the thermal shock resistance of ceramic tools in high-speed cutting engineering and meets the requirements of large temperature range during the machining of nickel-based superalloys. Moreover, the composite material does not use metal binder and has strong thermal stability even in the high-speed machining under extreme heat-force-chemistry coupling, so it has a high machining compatibility for nickel-based superalloys.

公开(公告)号：US20230405187A1

公开(公告)日：2023-12-21

申请号：US17939524

申请日：2022-09-07

Applicant: YANSHAN UNIVERSITY ,  SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Xu HAN ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Dun LIU ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG

IPC: A61L27/52 ,  C08J3/075 ,  C12N5/00 ,  A61L27/26 ,  B33Y70/00 ,  B33Y80/00 ,  B33Y40/00 ,  B33Y10/00

CPC classification number: A61L27/52 ,  C08J3/075 ,  C12N5/0062 ,  A61L27/26 ,  B33Y70/00 ,  B33Y80/00 ,  B33Y40/00 ,  B33Y10/00 ,  C08J2489/04 ,  C08J2301/28 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/78

Abstract: A composite hydrogel for light-cured 3D cell-laden printing and a preparation method and application thereof. The composite hydrogel of the present disclosure combines advantages of gelatin methacryloyl, sodium carboxymethylcellulose, hyaluronic acid-glutamic acid polymer, and the like. The provided composite hydrogel for 3D printing has the characteristics of low toxicity, good biocompatibility and adjustable mechanical properties, can provide cells with a three-dimensional living environment, promotes cell adhesion and migration on gradient scaffolds, and is suitable for tissue engineering scaffolds and cell-laden printing of tissues. The printing process of a scaffold is simple and can be completed within a short time, and the porosity and mechanical properties of the 3D printed hydrogel scaffold can be adjusted by adjusting the proportion of HA-Glu and Col in the hydrogel system.

公开(公告)号：US20230303453A1

公开(公告)日：2023-09-28

申请号：US17943596

申请日：2022-09-13

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Xinyao CUI ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Xiaolan BAI ,  Zhen WANG ,  Longhua XU ,  Dun LIU ,  Shuiquan HUANG ,  Hongtao ZHU ,  Bin ZOU

IPC: C04B35/58 ,  C04B35/626 ,  C04B35/645

CPC classification number: C04B35/58021 ,  C04B35/6261 ,  C04B35/645 ,  C04B35/6264 ,  C04B2235/3886 ,  C04B2235/3843 ,  C04B2235/3891 ,  C04B2235/405 ,  C04B2235/404 ,  C04B2235/75 ,  C04B2235/786 ,  C04B2235/785 ,  C04B2235/604 ,  C04B2235/6562 ,  C04B2235/6567 ,  B26D2001/002

Abstract: A crack self-healing functionally gradient material for ceramic cutting tools and a preparation method thereof. The material for ceramic cutting tools has a symmetrical gradient structure, and based on the percentage by mass, components of each layer include 50%-80% of Ti(C7,N3), 25%-5% of (W7,Ti3)C and 20%-0% of TiSi2; contents of components of layers that are symmetrical relative to a central layer are the same and a thickness is symmetrically distributed; a content of Ti(C7,N3) gradually increases from the surface layer to the central layer, contents of (W7,Ti3)C and Ti Si2 gradually decrease by 5% from the surface layer to the central layer, and the contents of Ni and Mo gradually increase from the surface layer to the central layer.

公开(公告)号：US20230279345A1

公开(公告)日：2023-09-07

申请号：US17851731

申请日：2022-06-28

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhichao WANG ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Dun LIU ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG ,  Minting WANG ,  Hongtao ZHU ,  Bin ZOU

IPC: C12N5/00 ,  B33Y10/00 ,  B33Y80/00 ,  B29C64/106

CPC classification number: C12N5/0068 ,  B33Y10/00 ,  B33Y80/00 ,  B29C64/106 ,  C12N2533/30 ,  B29L2031/7534

Abstract: A vascular structure-containing large-scale biological tissue and a construction method thereof. In the existing three-dimensional cell culture, it is contradictory for the elastic modulus of the scaffold material in ensuring structural stability and biocompatibility, and the vascular structure is required to provide channels for nutrient exchange when a large-scale structure is prepared. A cell-laden matrix material is poured into a hollow scaffold serving as a supporting scaffold. The overall stability of the scaffold structure can be ensured by regulating the mechanical properties of the supporting scaffold, thereby resolving the contradiction in ensuring structural stability and biocompatibility for the mechanical properties of the scaffold material in the conventional three-dimensional cell culture. A coaxially printing outer material contains a thermosensitive material. The removal of the outer thermosensitive material can increase the porosity of the vascular walls, and further increase the diffusion in the hollow vascular ducts.

公开(公告)号：US20230166342A1

公开(公告)日：2023-06-01

申请号：US17762960

申请日：2021-04-12

Applicant: SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhen CHEN ,  Hanlian LIU ,  Hongtao ZHU ,  Bin ZOU ,  Peng YAO ,  Jun WANG

IPC: B23C5/26

CPC classification number: B23C5/26 ,  B23C2210/0407 ,  B23C2210/54

Abstract: A milling machine fly-cutter with adjustable cutting-tool geometric angle and milling machine includes a fly-cutter plate body, a plurality of combination grooves are evenly set in the circumferential direction of the fly-cutter plate body, and adjusting assemblies are mounted in the combination grooves. Wherein, the adjusting assemblies include an adjusting block for rake angle and relief angle, an adjusting block for tool cutting edge angle and minor cutting edge angle, an adjusting block for tool cutting edge inclination angle. A first side of the adjusting block is attached with a cutter handle, and a second side is connected with the adjusting block for rake angle and relief angle by the adjusting block for tool cutting edge angle and minor cutting edge angle. The milling machine fly-cutter realizes adjustment of the geometric angles of cutting tool, and is able to adapt to cutter handles of a variety of different specifications.

公开(公告)号：US20220404238A1

公开(公告)日：2022-12-22

申请号：US17618692

申请日：2021-01-15

Applicant: SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Binghao LI ,  Hanlian LIU ,  Hongtao ZHU ,  Bin ZOU ,  Peng YAO ,  Jun WANG

IPC: G01N1/08 ,  B23B49/00

Abstract: A BTA drilling quick-stop device (QSD) and a method, wherein the BTA drilling QSD includes a base. The base includes a hollow cavity. A side portion of the base has an opening communicating with the hollow cavity. The opening is closed by a through cover. A horizontally movable fixture is disposed in the hollow cavity. A stopper is disposed at a top portion of the base. A bottom portion of the stopper is disposed lower than a top portion of the fixture to stop the fixture from moving during processing. An elastic element is disposed between the through cover and a side portion of the fixture to push the fixture to move after processing.

公开(公告)号：US20250002660A1

公开(公告)日：2025-01-02

申请号：US18692195

申请日：2022-01-11

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhichao WANG ,  Hanlian LIU ,  Peng YAO ,  Wei WANG ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG ,  Jun WANG ,  Hongtao ZHU ,  Bin ZOU

IPC: C08J3/075 ,  B33Y80/00 ,  C08J3/28 ,  C08J9/26 ,  C08J9/28 ,  C12N5/00

Abstract: A method for prepring a porous aerogel scaffold includes: adding a photoinitiator and polyethylene glycol diacrylate in a buffer solution, dissolving by heating and evenly mixing, adding Pluronic F127 into the mixed solution, and standing at a low temperature to obtain an aerogel scaffold material; printing a hydrogel scaffold by using a 3D printing technology, and performing UV irradiation so that a cross-linking of the hydrogel scaffold is caused to form a three-dimensional scaffold with a stable structure, performing low-temperature soaking to remove Pluronic F127, and then freeze drying the three-dimensional scaffold to obtain the porous aerogel scaffold. Wherein, Pluronic F127 serves as a sacrificial material which is removed after the 3D printing of the hydrogel scaffold is completed, and then a porous structure can be formed in the scaffold in combination with a freeze drying technology, which facilitates the survival, growth and proliferation of cells during the three-dimensional culture.