摘要:
Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要:
Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要:
A DNA chip includes a large number of minute spots formed by dripping sample solutions onto a base plate. The base plate is provided with positional deviation-correcting means for displacing the spots and automatically correcting any positional deviation of each of the spots. The positional deviation-correcting means includes any one of at least one projection, at least one recess and electric field-generating means for providing charged states on the base plate centered at a correct position for each of the spots supplied on the base plate.
摘要:
A liquid-drop discharge device includes an array of liquid-drop discharge units for discharging liquid from a float cell in communication with a liquid storage tank. A reaction cell, into which fluid is discharged, is provided in communication with the float cell. A differential adjusting tube is provided between the float cell and the reaction cell and includes a release valve for maintaining a specified pressure relationship between an internal pressure P2 of the float cell and an internal pressure P3 of the reaction cell. A driving circuit is provided to drive a pressurizing source of the liquid-drop discharge device and is connected to a control unit. The control unit is connected to a pressure sensor, which detects the internal pressure of the reaction cell and the release valve. The internal pressure P1 of the liquid storage tank is controlled to be larger than the internal pressure P3 of the reaction cell.
摘要:
In a method for producing a biochip comprising a large number of spots based on captures, the spots are arranged on a base plate 10 by supplying, onto the base plate 10, a plurality of types of the captures to be used to specifically react with a specimen in order to obtain information on a structure or a function of the specimen, an immobilization-reinforcing solution 16 is supplied onto the base plate 10 (first supply step). Subsequently, a sample 14, which is obtained in a sample preparation step, is supplied onto the immobilization-reinforcing solution 16 having been supplied onto the base plate 10 to produce the biochip thereby (second supply step).
摘要:
A dispenser includes a plurality of arranged micropipettes, each including a sample-pouring port for pouring a sample solution from the outside, a cavity for pouring and charging the sample solution thereinto, and a sample discharge port for discharging the sample solution, and formed on at least one or more substrates. The micropipette further includes an actuator section disposed on at least one wall surface of the substrate which forms the cavity so that the sample solution is movable in the cavity, and the sample solution is discharged from the sample discharge port of each of the micropipettes. A pin, which protrudes upwardly, is provided at the sample-pouring port of each of the micropipettes.
摘要:
A liquid-drop discharge device is provided including a float cell provided with a valve and communicating to a liquid storage tank, a liquid discharge mechanism for discharging liquid within the float cell, and a reaction cell provided with a space into which fluid is discharged from the liquid discharge mechanism for collecting the discharged fluid. Air is supplied to the reaction cell for collecting liquid or minute particles, and an internal pressure P1 of the liquid storage tank or the float cell and an internal pressure P3 of the reaction cell are set such that a relationship of P1=P3, in which the pressures are identical to atmospheric pressure, or P1>P3, to supply stronger air, is satisfied.
摘要:
A micropipette is provided including at least one substrate, an inlet port through which a sample is delivered from the outside, a cavity to be poured and filled with the sample, an introduction hole, and an injection port for expelling the sample are formed on the at least one substrate. The substrate for forming the cavity is made of ceramics, a piezoelectric/electrostrictive element is provided for at least one wall surface of the substrate, and the sample moves as a laminar flow in the cavity. The volume of the cavity is changed by driving the piezoelectric/electrostrictive element to expell a certain amount of the sample in the cavity from the injection port. According to the micropipette, it is possible to form microspots with high accuracy and high speed. According to a dispenser including the micropipette, it is possible to efficiently dispense hundreds to ten thousands of different samples at one time and form microspots. Therefore, productivity is remarkably improved.
摘要:
A micropipette is provided including at least one substrate, an inlet port through which a sample is delivered from the outside, a cavity to be poured and filled with the sample, an introduction hole, and an injection port for expelling the sample are formed on the at least one substrate. The substrate for forming the cavity is made of ceramics, a piezoelectric/electrostrictive element is provided for at least one wall surface of the substrate, and the sample moves as a laminar flow in the cavity. The volume of the cavity is changed by driving the piezoelectric/electrostrictive element to expell al certain amount of the sample in the cavity from the injection port. According to the micropipette, it is possible to form microspots with high accuracy and high speed. According to a dispenser including the micropipette, it is possible to efficiently dispense hundreds to ten thousands of different samples at one time and form microspots. Therefore, productivity is remarkably improved.
摘要:
A recording head including an electrically insulating layer, a plurality of recording electrodes and at least one return circuit electrode provided on the opposite sides of the insulating layer, the insulating layer and electrodes being adapted to be held, at a distal end of the head, in contact with the resistive layer. The electrically insulating layer has a surface waviness represented by maximum filtered waviness, of not larger than 0.40 .mu.m, where a high band cut-off value is 0.8 mm and a reference length is 2.5 mm, as measured in main scanning direction perpendicular to a stacking direction of the electrically insulating layer, the recording and return circuit electrodes. The insulating layer also has a surface roughness represented by center-line mean roughness, within a range of 0.02 .mu.m to 0.4 .mu.m, where a cut-off value is 0.8 mm and a measuring length is 2.5 mm, as measured in the main scanning direction.