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公开(公告)号：US06465190B1
公开(公告)日：2002-10-15
申请号：US09694135
申请日：2000-10-23
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译： 公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤， 以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。 样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤 。
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公开(公告)号：US06649343B1
公开(公告)日：2003-11-18
申请号：US09693228
申请日：2000-10-20
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/0819 , B01L2400/0415 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/00237 , G01N2035/1039 , C12Q2565/507
摘要： A DNA chip includes a large number of minute spots formed by dripping sample solutions onto a base plate. The base plate is provided with positional deviation-correcting means for displacing the spots and automatically correcting any positional deviation of each of the spots. The positional deviation-correcting means includes any one of at least one projection, at least one recess and electric field-generating means for providing charged states on the base plate centered at a correct position for each of the spots supplied on the base plate.
摘要翻译： DNA芯片包括通过将样品溶液滴落到基板上而形成的大量微小斑点。 基板设置有用于移动斑点并自动校正每个斑点的任何位置偏差的位置偏差校正装置。 位置偏差校正装置包括至少一个突起，至少一个凹部和电场产生装置中的任何一个，用于在基板上以提供在基板上的每个点的正确位置为中心提供充电状态。
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公开(公告)号：US06554405B1
公开(公告)日：2003-04-29
申请号：US09669933
申请日：2000-09-26
IPC分类号： B41J2045
CPC分类号： B01J4/008 , B01J2219/00162 , B01J2219/002 , B01J2219/00213 , B01J2219/00231 , B01J2219/0027 , B41J2/175
摘要： A liquid-drop discharge device includes an array of liquid-drop discharge units for discharging liquid from a float cell in communication with a liquid storage tank. A reaction cell, into which fluid is discharged, is provided in communication with the float cell. A differential adjusting tube is provided between the float cell and the reaction cell and includes a release valve for maintaining a specified pressure relationship between an internal pressure P2 of the float cell and an internal pressure P3 of the reaction cell. A driving circuit is provided to drive a pressurizing source of the liquid-drop discharge device and is connected to a control unit. The control unit is connected to a pressure sensor, which detects the internal pressure of the reaction cell and the release valve. The internal pressure P1 of the liquid storage tank is controlled to be larger than the internal pressure P3 of the reaction cell.
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公开(公告)号：US07407746B2
公开(公告)日：2008-08-05
申请号：US10068292
申请日：2002-02-06
IPC分类号： C12Q1/68
CPC分类号： G01N33/54366 , B01J19/0046 , B01J2219/00351 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00722 , B01L3/5088 , B82Y5/00 , C12Q1/6837 , G01N33/54353
摘要： In a method for producing a biochip comprising a large number of spots based on captures, the spots are arranged on a base plate 10 by supplying, onto the base plate 10, a plurality of types of the captures to be used to specifically react with a specimen in order to obtain information on a structure or a function of the specimen, an immobilization-reinforcing solution 16 is supplied onto the base plate 10 (first supply step). Subsequently, a sample 14, which is obtained in a sample preparation step, is supplied onto the immobilization-reinforcing solution 16 having been supplied onto the base plate 10 to produce the biochip thereby (second supply step).
摘要翻译： 在基于捕获的包含大量斑点的生物芯片的制造方法中，通过向基板10上提供多种类型的捕捉物来将斑点布置在基板10上，以用于与 试样为了获得关于试样的结构或功能的信息，将固定补强溶液16供给到基板10上（第一供给步骤）。 随后，将在样品制备步骤中获得的样品14供给到已经供应到基板10上的固定补强溶液16，从而生成生物芯片（第二供给步骤）。
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公开(公告)号：US06814937B1
公开(公告)日：2004-11-09
申请号：US09694130
申请日：2000-10-23
IPC分类号： B01L302
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00659 , B01J2219/00722 , B01L3/022 , B01L3/0241 , B01L3/0268 , B01L2200/027 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： A dispenser includes a plurality of arranged micropipettes, each including a sample-pouring port for pouring a sample solution from the outside, a cavity for pouring and charging the sample solution thereinto, and a sample discharge port for discharging the sample solution, and formed on at least one or more substrates. The micropipette further includes an actuator section disposed on at least one wall surface of the substrate which forms the cavity so that the sample solution is movable in the cavity, and the sample solution is discharged from the sample discharge port of each of the micropipettes. A pin, which protrudes upwardly, is provided at the sample-pouring port of each of the micropipettes.
摘要翻译： 分配器包括多个布置的微量移液器，每个微量移液器包括用于从外部倾倒样品溶液的样品注入口，用于将样品溶液倒入和填充的腔体，以及用于排出样品溶液的样品排出口，并形成在 至少一个或多个基底。 微量移液管还包括设置在基板的至少一个壁表面上的致动器部分，其形成空腔，使得样品溶液可在空腔中移动，并且样品溶液从每个微量移液管的样品排出口排出。 在每个微量移液管的取样口处设置有向上突出的销。
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公开(公告)号：US06776960B2
公开(公告)日：2004-08-17
申请号：US10128777
申请日：2002-04-24
IPC分类号： G01N1506
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译： 公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤， 以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。 样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤 。
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公开(公告)号：US06443366B1
公开(公告)日：2002-09-03
申请号：US09685492
申请日：2000-10-10
IPC分类号： B05B108
CPC分类号： B41J2/02
摘要： A liquid-drop discharge device is provided including a float cell provided with a valve and communicating to a liquid storage tank, a liquid discharge mechanism for discharging liquid within the float cell, and a reaction cell provided with a space into which fluid is discharged from the liquid discharge mechanism for collecting the discharged fluid. Air is supplied to the reaction cell for collecting liquid or minute particles, and an internal pressure P1 of the liquid storage tank or the float cell and an internal pressure P3 of the reaction cell are set such that a relationship of P1=P3, in which the pressures are identical to atmospheric pressure, or P1>P3, to supply stronger air, is satisfied.
摘要翻译： 本发明提供一种液滴放电装置，包括具有阀并与液体储存罐连通的浮子池，用于在浮子池内排出液体的液体排出机构，以及设置有流体从其排出的空间的反应池 用于收集排出的流体的液体排出机构。 将空气供给到用于收集液体或微小颗粒的反应池中，并且将液体储存罐或浮子池的内部压力P1和反应池的内部压力P3设定为P1 = P3的关系，其中 压力与大气压力相同，或P1> P3，以提供更强的空气。
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公开(公告)号：US20050145032A1
公开(公告)日：2005-07-07
申请号：US11060988
申请日：2005-02-18
申请人： Yukihisa Takeuchi , Takao Ohnishi , Koji Kimura
发明人： Yukihisa Takeuchi , Takao Ohnishi , Koji Kimura
IPC分类号： B06B1/06 , G01G3/16 , G01H11/08 , G01N35/00 , G01P15/09 , G02B26/02 , H01L41/09 , H01L41/113 , H01L41/187 , G01H13/00 , G01G3/13 , G01G9/00
CPC分类号： G01P15/0922 , B06B1/06 , B06B1/0648 , G01G3/16 , G01H11/08 , G01N2035/00217 , G01N2035/00237 , G01N2291/02827 , G01P15/09 , G01P2015/0814 , H01L41/0973 , H01L41/1132
摘要： A mass sensor in which a connection plate and a diaphragm are joined together at their respective sides; a sensing plate is joined to the connection plate at their respective sides in the direction perpendicular to the direction where the diaphragm is joined to the connection plate; a piezoelectric element consisting of a piezoelectric film and an electrode is installed on at least either one of plate surfaces of the sensing plate; and a resonating portion consisting of the diaphragm, the sensing plate, the connection plate, and the piezoelectric element is joined to a sensor substrate. Change in the mass of the diaphragm is measured by measuring change in the resonant frequency of the resonating portion accompanying the change in the mass of the diaphragm.
摘要翻译： 质量传感器，其中连接板和隔膜在其各自的侧面接合在一起; 感测板在垂直于隔膜接合方向的连接板的相应侧面处连接到连接板; 由压电膜和电极构成的压电元件安装在感测板的板表面的至少任一个上; 并且由隔膜，感测板，连接板和压电元件组成的谐振部分接合到传感器基板。 通过测量伴随隔膜质量变化的谐振部分的谐振频率的变化来测量隔膜质量的变化。
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公开(公告)号：US20050016277A1
公开(公告)日：2005-01-27
申请号：US10925514
申请日：2004-08-25
申请人： Yukihisa Takeuchi , Takao Ohnishi , Koji Kimura
发明人： Yukihisa Takeuchi , Takao Ohnishi , Koji Kimura
IPC分类号： B06B1/06 , G01G3/16 , G01H11/08 , G01N35/00 , G01P15/09 , G02B26/02 , H01L41/09 , H01L41/113 , H01L41/187 , G01H13/00
CPC分类号： G01P15/0922 , B06B1/06 , B06B1/0648 , G01G3/16 , G01H11/08 , G01N2035/00217 , G01N2035/00237 , G01N2291/02827 , G01P15/09 , G01P2015/0814 , H01L41/0973 , H01L41/1132
摘要： A mass sensor in which a connection plate and a diaphragm are joined together at their respective sides; a sensing plate is joined to the connection plate at their respective sides in the direction perpendicular to the direction where the diaphragm is joined to the connection plate; a piezoelectric element consisting of a piezoelectric film and an electrode is installed on at least either one of plate surfaces of the sensing plate; and a resonating portion consisting of the diaphragm, the sensing plate, the connection plate, and the piezoelectric element is joined to a sensor substrate. Change in the mass of the diaphragm is measured by measuring change in the resonant frequency of the resonating portion accompanying the change in the mass of the diaphragm.
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公开(公告)号：US06457361B1
公开(公告)日：2002-10-01
申请号：US09387012
申请日：1999-08-31
申请人： Yukihisa Takeuchi , Takao Ohnishi , Koji Kimura
发明人： Yukihisa Takeuchi , Takao Ohnishi , Koji Kimura
IPC分类号： G01H1300
CPC分类号： G01G3/13
摘要： A mass sensor including: a connecting plate having one or more slit(s) and/or opening portion(s) formed therein and/or having a thin-walled portion and a thick-walled portion formed therein; a diaphragm joined with the connecting plate at respective side surfaces; a piezoelectric element; a sensing plate with the piezoelectric element being provided at least at one part on at least one surface of the sensing plate, which has its side surface joined with a side surface of the connecting plate in the direction perpendicular to the joining direction of the diaphragm and the connecting plate; and a sensor substrate with which at least a part of side surfaces of the connecting plate as well as the sensing plate are joined, and the diaphragm, the connecting plate, the sensing plate, and the piezoelectric element form a resonating portion. The mass sensor can conveniently be used for determining the mass of a substance to be sensed by measuring changes in resonant frequencies caused by changes in the mass of the diaphragm on which a catching substance for catching a substance to be sensed by reacting only with the object of sensing is applied.
摘要翻译： 一种质量传感器，包括：连接板，其中形成有一个或多个狭缝和/或开口部分，和/或具有形成在其中的薄壁部分和厚壁部分; 在相应的侧表面处连接有连接板的隔膜; 压电元件; 具有压电元件的检测板至少在传感板的至少一个表面上的一个部分上设置，其侧表面在垂直于隔膜的接合方向的方向上与连接板的侧表面接合， 连接板; 以及传感器基板，连接板的至少一部分的侧面以及感测板与其连接，隔膜，连接板，感测板和压电元件形成谐振部。 质量传感器可以方便地用于通过测量由通过仅与物体反应而捕获待检测物质的捕获物质的膜片的质量变化引起的谐振频率的变化来确定待感测物质的质量 的感应。
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