公开(公告)号：US20230340557A1

公开(公告)日：2023-10-26

申请号：US18016581

申请日：2021-07-12

申请人： BIOMILLENIA SAS

发明人： Aleksander Dajkovic ,  Guansheng Du ,  Dirk Loeffert

IPC分类号： C12Q1/02 ,  C12Q1/20 ,  B01L3/00 ,  B01L7/00 ,  C12M3/06 ,  C12M1/34

CPC分类号： C12Q1/025 ,  C12Q1/20 ,  B01L3/502784 ,  B01L7/52 ,  C12M23/16 ,  C12M41/36 ,  B01L2300/0829 ,  B01L2300/18 ,  B01L2200/0647

摘要： The present invention uses microfluidics droplets for studying the growth dynamics of communities of bacteria in the presence of various perturbations such as various chemicals or other microorganisms and to establish microbial assemblies model which is representative of a microbiota of interest and to examine their growth dynamics after perturbations such as with an array of chemicals or other microorganisms. In particular, the present invention refers to a method and apparatus for assessing the effect of a chemical or biological substance on the growth of microorganisms.

公开(公告)号：US20230183774A1

公开(公告)日：2023-06-15

申请号：US18064511

申请日：2022-12-12

申请人： Georgia Tech Research Corporation

发明人： Robert Martin Dickson ,  Alexandra B. Filbrun ,  Prakash C. Khanal

IPC分类号： C12Q1/20 ,  G01N21/78 ,  B01L3/00 ,  C12Q1/04

CPC分类号： C12Q1/20 ,  G01N21/78 ,  B01L3/5085 ,  C12Q1/04 ,  B01L2300/0829 ,  B01L2300/0654

摘要： An exemplary embodiment of the present disclosure provides a system for detecting antimicrobial resistance of a bacteria in a biological sample. In some embodiments, the system can include a plurality of containers, a detecting agent in each of the plurality of containers, and an antimicrobial agent in at least a portion of the plurality of containers. The antimicrobial agent is disposed in at least one of the plurality of containers. Each of the containers can contain at least a portion of the biological sample. The detecting agent can be configured to produce optically detectable changes responsive to bacterial respiration or growth, directly from patient samples of from patient sample cultures.

公开(公告)号：US20230110491A1

公开(公告)日：2023-04-13

申请号：US17908886

申请日：2021-03-08

申请人： Biosynth AG

发明人： Julian IHSSEN ,  Urs SPITZ

IPC分类号： C12Q1/20 ,  C12M1/32 ,  C12Q1/66

摘要： The present invention relates to a method for assessing cell viability of bacterial and fungal cells and to a method for the detection of bacterial and fungal cells with specific enzyme activities. The methods of the present invention rely on the real-time measurement of the level of luminescence signal from a luciferase enzyme directly from a growing culture of bacterial or fungal cells. Furthermore, the present invention relates to a method for assessing susceptibility of bacterial cells to antibiotics by measuring ATP levels using a luciferase assay system.

公开(公告)号：US20220383550A1

公开(公告)日：2022-12-01

申请号：US17776137

申请日：2020-09-29

申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Maya OZAKI ,  Yoko INOUE ,  Sachiko YAMADA ,  Ryoko MATSUYAMA ,  Hanaho KANO

IPC分类号： G06T7/90 ,  G06T7/60 ,  G06T7/00 ,  C12Q1/20 ,  B01L3/00 ,  G01N21/27 ,  G01N21/78

摘要： A method for assessing an assessment target according to an embodiment includes: a step of acquiring statistical information based on at least one color feature quantity with respect to each of a plurality of assessment regions in a plate image corresponding to an image of an assessment plate that holds an assessment target in a plurality of wells provided in the plate; and a step of determining a color of the plurality of assessment regions by using the statistical information. The assessment target includes a tester, the plurality of wells include a test substance well holding the assessment target that further includes a test substance, the plate image includes a plurality of well images corresponding to the plurality of wells, and each of the plurality of assessment regions includes at least one well image corresponding to at least one of the wells.

公开(公告)号：US11185860B2

公开(公告)日：2021-11-30

申请号：US16217602

申请日：2018-12-12

申请人： Wisconsin Alumni Research Foundation

发明人： David J. Beebe ,  Duane S. Juang ,  Jiaquan Yu ,  Jo Handelsman

IPC分类号： B01L3/00 ,  C12Q1/20 ,  C40B60/12

摘要： A platform and method for conducting multi-variable combinational interactions are provided. An array of multiplexing chambers in formed in a body. The body also includes a common well communicating with each multiplexing chamber of the array of multiplexing chambers and a plurality of variable wells. Each of variable wells communicates with at least one multiplexing chamber of the array of multiplexing chambers. The common well is loaded with a first variable and different variables are loaded in each of the plurality of variable wells. The interaction of the first variable with at least one of the different variables in each multiplexing chamber of the array of multiplexing chambers is observed.

公开(公告)号：US10415074B2

公开(公告)日：2019-09-17

申请号：US15731735

申请日：2016-01-25

申请人： Charles Cervin

发明人： Charles Cervin

IPC分类号： C12Q1/00 ,  C12N1/00 ,  C12Q1/20 ,  C12Q1/04 ,  C12Q1/66 ,  G01N21/76 ,  G01N33/14

摘要： A method for detecting lactic acid and/or acetic acid bacteria in a food-processing matrix is taught, using microbial flora including a lactic acid and/or acetic acid bacterial flora and a fungal flora. The bacterial flora contains an adenosine triphosphate of bacterial origin, and the fungal flora contains an adenosine triphosphate of fungal origin. The method comprises applying, to the matrix, before a first time limit, an antifungal having an antifungal action which is lethal, on the fungal flora, and at a second time limit, an antibiotic action which is non lethal, at a second time limit after the first time limit, on the bacterial flora. The microbial flora is detected between the first time limit and the second time limit; the lethal antifungal action releases, into the matrix, for the first time limit, adenosine triphosphate of fungal origin and in which the microbial flora is detected between the first time limit and the second time limit.

公开(公告)号：US20190276871A1

公开(公告)日：2019-09-12

申请号：US15887794

申请日：2018-02-02

申请人： SeLux Diagnostics, Inc.

发明人： Eric Stern ,  Kelly Flentie ,  Aleksandar Vacic ,  Frederick P. Floyd, JR. ,  Sarah A. Scott

IPC分类号： C12Q1/20 ,  C12M3/00 ,  C12M1/32 ,  C12M1/34 ,  C12M1/36

摘要： The present invention provides, among other things, a master cartridge for effective storage and transportation of antimicrobials, the master cartridge comprising multiple reservoir units for placing a plurality of antimicrobials at high concentration from which multiple patient cartridges could be generated for testing a plurality of biological samples, and methods for using the same.

公开(公告)号：US20190249219A1

公开(公告)日：2019-08-15

申请号：US15767481

申请日：2016-10-12

申请人： CureVac AG

发明人： Isabel REICHERT ,  Fabian Johannes EBER ,  Christian MAYER ,  Aniela WOCHNER

IPC分类号： C12Q1/24 ,  C12Q1/20

CPC分类号： C12Q1/24 ,  C12N1/20 ,  C12Q1/20

摘要： The present invention is concerned with an automated method for obtaining at least one discrete colony from microorganisms or cells comprised in a solution. This method includes a step where acoustic liquid transfer is employed. The present invention further relates to the use of an acoustic liquid transfer device for obtaining at least one discrete colony from micro organisms or cells comprised in a solution. The present invention also relates to an automated method for determining the presence and/or quantity of microorganisms or cells potentially comprised in a solution and to the use of an acoustic liquid transfer device for determining the presence and/or quantity of microorganisms or cells potentially comprised in a solution.

公开(公告)号：US09994890B1

公开(公告)日：2018-06-12

申请号：US13770532

申请日：2013-02-19

申请人： Northeastern University

发明人： Slava Epstein

IPC分类号： C40B30/06 ,  C12Q1/20 ,  G01N33/50

CPC分类号： C12Q1/18 ,  C40B30/06

摘要： Disclosed are methods of identifying compounds that suppress the growth of target cells. In certain embodiments, the methods comprise providing a panel of target cells and the panel of target cells comprises a plurality of populations of target cells. Each population comprises cells that are resistant to one or more compounds known to suppress the growth of cells sensitive to the one or more compounds. Each population of target cells grows in the presence of a candidate compound and a determination of whether the candidate compound suppresses the growth of one or more populations of target cells.

公开(公告)号：US09637771B2

公开(公告)日：2017-05-02

申请号：US14472144

申请日：2014-08-28

申请人： Institute for Environmental Health, Inc.

发明人： Mansour Samadpour

IPC分类号： C12Q1/06 ,  C12Q1/08 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/20 ,  C12Q1/22 ,  C12Q1/24 ,  C12Q1/04 ,  C12Q1/18 ,  G01N1/28 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/573 ,  G01N33/533 ,  C12M1/00

CPC分类号： C12Q1/10 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/28 ,  Y10S435/849

摘要： Particular aspects provide a method of sampling, testing and validating test lots (e.g., single-unit production lots), comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the collected product portions to provide a corresponding set of test lot samples (wherein each test lot sample is attributed to a particular corresponding test lot); enriching the set of test lot samples; removing equal portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample for the target agent/organism, wherein where such testing is positive, individual test lots may nonetheless yet be validated by further testing of a respective enriched test lot sample and obtaining a negative test result. The methods have broad utility for monitoring all sorts of test lots (e.g., environmental lots, production lots, pharmaceutical lots, etc.) and for efficiently affecting informed, targeted remedial measures.