公开(公告)号：US20160082366A1

公开(公告)日：2016-03-24

申请号：US14764179

申请日：2014-01-10

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Qiang YANG ,  Xiao XU ,  Hao LU ,  Chaoyang WANG

IPC: B01D19/00

CPC classification number: B01D19/0052 ,  B01D19/0036 ,  B01D19/0057

Abstract: The present invention relates to a device for liquid degassing by means of the swirling or the centrifugal field coupled with the pressure gradient field. This device comprises a cavity. The cavity is furnished with an inlet for liquid gas, an outlet for gas phase and an outlet for gas liquid phase. The outlet for gas phase is inserted into the cavity through the upper surface of the cavity, wherein the depth of the insertion is around 0.1-3 times of the maximum diameter of the cavity. Specifically, the outlet for gas phase is realized through an overflow pipe with the diameter gradually increasing from the bottom to the top. The end of the overflow pipe is further furnished with a bell mouth. The advantages of the present invention are: The present invention designs the structure of an inverted cone shaped outlet for gas phase which is inserted into the cavity of the swirling device. The present invention makes full use of the impact of pressure gradient in the cavity on the solubility of gas in liquid and combines the centrifugal field and the pressure gradient field to remove the gas entrained with liquid while the dissolved gas is removed under the inlet partial pressure.

Abstract translation: 本发明涉及通过与压力梯度场耦合的涡流或离心场进行液体脱气的装置。 该装置包括空腔。 空腔配有液体气体入口，气相出口和气液相出口。 用于气相的出口通过空腔的上表面插入空腔中，其中插入深度为空腔最大直径的0.1-3倍。 具体来说，气相出口通过从底部到顶部逐渐增加的溢流管实现。 溢流管的末端还配有喇叭口。 本发明的优点是：本发明设计了插入旋流装置的空腔中的用于气相的倒锥形出口的结构。 本发明充分利用空腔内的压力梯度对液体中气体溶解度的影响，结合离心场和压力梯度场来除去在入口分压下去除溶解气体时液体夹带液体 。

公开(公告)号：US20160040173A1

公开(公告)日：2016-02-11

申请号：US14419106

申请日：2013-08-01

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： YI YANG ,  XIANJUN CHEN ,  ZHENGCAI MA ,  RENMEI LIU

IPC: C12N15/63 ,  C12N15/70 ,  C12N13/00

CPC classification number: C12N15/635 ,  C12N9/0051 ,  C12N13/00 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12P21/02

Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium.

Abstract translation: 提供了一种原核细菌的光学控制基因表达系统，其包含：a）感光重组转录因子编码基因，所述感光重组转录因子是包含第一多肽作为DNA结合结构域的第一种融合蛋白，第二多肽作为感光结构域 ; b）包含启动子或启动子 - 反应元件或含有至少一个被第一多肽识别/结合的反应元件和待转录的核酸序列的反应元件的靶转录单元。 还提供了包含所述光学控制的基因表达系统的原核表达载体和通过使用光学控制的基因表达系统调节原核宿主细胞中的基因表达的方法。 还提供了含有光学控制的基因表达系统的不同成分的试剂盒。 原核细菌的光学控制基因表达系统具有快速，有效和强大的诱导，比其他诱导物更安全，毒性很小或无毒，可以在空间和时间上控制基因表达，并且可以调节原核细菌的许多生命过程 。

公开(公告)号：US20150299716A1

公开(公告)日：2015-10-22

申请号：US14413930

申请日：2012-11-15

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Xiangshan Zhou ,  Yuanxing Zhang ,  Jinjia Wang ,  Xiaolong Wang ,  Peng Bai ,  Ping Zhang

IPC: C12N15/81 ,  C12P21/00

CPC classification number: C12N15/815 ,  C07K14/39 ,  C12N1/32 ,  C12P21/00

Abstract: Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

Abstract translation: 提供了消除甲醇诱导的启动子对用于表达外来多肽的单一甲醇碳源的依赖性的方法。 该方法包括通过增加甲基营养酵母细胞中Mit1多肽的表达量来激活需要甲醇诱导的启动子的表达，使得最初依赖于甲醇诱导的启动子不再取决于单一甲醇，并且还可以表达外源多肽。

公开(公告)号：US20150252391A1

公开(公告)日：2015-09-10

申请号：US14417166

申请日：2013-09-26

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY ,  JIAXING ZEYUAN BIO-PRODUCTS CO., LTD. ,  SHANGHAI ZEYUAN MARINE BIOTECHNOLOGY CO., LTD.

Inventor： Yuanguang Li ,  Zhen Zhang ,  Jianhua Fan ,  Minxi Wan ,  Dongmei Hou ,  Jingkui Zhang ,  Jianke Huang ,  Songtao Liang ,  Jun Wang ,  Jie Chen ,  Weiliang Wang ,  Jun Wang ,  Shulan Li ,  Guomin Shen

IPC: C12P7/26

CPC classification number: C12P7/26 ,  C12N1/12 ,  C12P23/00

Abstract: The present invention relates to a novel method for producing astaxanthin by using microalgae. The method comprises: heterotrophic cultivation of microalgae, dilution, photo-induction, collection of microalgal cells, and extraction of astaxanthin. The method according to the present invention takes full advantages of rapid growth rate in the heterotrophic stage and fast accumulation of astaxanthin in the photo-induction stage by using a large amount of microalgal cells obtained in the heterotrophic cultivation stage, so as to greatly improve the astaxanthin production rate and thereby achieve low cost, high efficiency, large scale production of astaxanthin by using microalgae. The method not only provides an important technical means to address the large scale industrial production of astaxanthin through microalgae but also ensures an ample source of raw material for the widespread utilization of astaxanthin.

Abstract translation: 本发明涉及一种利用微藻生产虾青素的新方法。 该方法包括：微藻的异养培养，稀释，光诱导，收集微藻细胞，提取虾青素。 根据本发明的方法，通过使用在异养培养阶段获得的大量微藻细胞，在异养阶段中快速生长速度和虾青素在光诱导阶段中的快速积累，具有很大的优势， 虾青素生产率，从而实现了低成本，高效率，大规模生产虾青素的微藻。 该方法不仅提供了解决微藻类大规模工业化生产虾青素的重要技术手段，同时也保证了大量虾青素的广泛应用。

公开(公告)号：US20150218470A1

公开(公告)日：2015-08-06

申请号：US14421042

申请日：2013-11-26

Applicant: East China University of Science and Technology ,  Praxair Technology, Inc.

Inventor： Qinghua Guo ,  Tao Wang ,  Zhenghua Dai ,  Guangsuo Yu ,  Xin Gong ,  Fuchen Wang ,  Haifeng Liu ,  Yifei Wang ,  William J. Mahoney ,  Sabuj Halder ,  Yi Ma ,  Jie Zhang ,  Zhijie Zhou ,  Robert J. Churpita ,  Christopher Herby ,  Jeffrey Wilson ,  Zhuoyong Yan

IPC: C10J3/80 ,  F23D14/22 ,  F23D14/32 ,  F23D14/66 ,  F02C3/28 ,  C07C29/04 ,  C01B3/02 ,  C01C1/02 ,  F01K23/10 ,  B05B1/00 ,  F23D14/78

CPC classification number: C10J3/80 ,  B05B1/005 ,  C01B3/02 ,  C01B3/363 ,  C01B2203/0255 ,  C01B2203/061 ,  C01B2203/068 ,  C01B2203/84 ,  C01C1/02 ,  C07C29/04 ,  C10J3/506 ,  C10J3/723 ,  C10J3/74 ,  C10J3/76 ,  C10J2200/152 ,  C10J2300/093 ,  C10J2300/0946 ,  C10J2300/0959 ,  C10J2300/0973 ,  C10J2300/1253 ,  C10J2300/1665 ,  C10J2300/1668 ,  C10J2300/1675 ,  C10J2300/1846 ,  F01K23/10 ,  F02C3/28 ,  F05D2220/32 ,  F05D2220/722 ,  F23D14/22 ,  F23D14/32 ,  F23D14/52 ,  F23D14/66 ,  F23D14/78 ,  F23D17/00 ,  F23N1/002 ,  F23R3/28 ,  F23R3/36 ,  Y02E20/18 ,  Y02E20/344

Abstract: A hot oxygen nozzle and uses thereof in a gasifier, the hot oxygen nozzle comprising an outer-ring spout, a middle-ring spout, an inner-ring spout, and a central spout all sequentially and coaxially disposed, and a cooling system; the gasifier is an entrained-flow gasifier provided with one or more nozzles on a certain plane or a plurality of planes at the top or on the periphery of the gasifier body. The nozzle has a simple structure and is easy to make and maintain. A fuel gas passage is disposed inside the nozzle. Oxygen can be heated by the combustion of fuel gas; and high-temperature and high-speed oxygen can directly ignite carbonaceous materials such as coal water slurry and coke oven gas. The present invention can be applied in a gasifier and then in the final process of synthesis gas preparation.

Abstract translation: 一种热氧喷嘴及其在气化器中的应用，所述热氧喷嘴包括顺序并同轴设置的外环喷口，中环喷口，内环喷口和中心喷口，以及冷却系统; 气化器是在气化器主体的顶部或周边的特定平面或多个平面上设置有一个或多个喷嘴的夹带气化器。 喷嘴结构简单，易于制作和维护。 燃料气体通道设置在喷嘴内。 氧气可以通过燃料气体的燃烧加热; 高温高温氧气可直接点燃煤泥浆和焦炉煤气等碳质材料。 本发明可以应用于气化器中，然后在最终的合成气制备过程中。

公开(公告)号：US20140372060A1

公开(公告)日：2014-12-18

申请号：US14365273

申请日：2012-11-27

Applicant: East China University of Science and Technology

Inventor： Fuzhen Xuan ,  Yupeng Cao ,  Hu Hui ,  Penning Li ,  Guozhen Wang

IPC: G01N3/62 ,  G01N3/40 ,  G06F17/50

CPC classification number: G01N3/62 ,  G01N3/40 ,  G01N2203/0067 ,  G01N2203/021 ,  G01N2203/0218 ,  G06F17/5018

Abstract: A calibration method for brittle fracture assessment parameters for pressure vessel materials based on the Beremin model includes selecting at least two types of specimens of different constraints, and calculating the fracture toughness values K0 corresponding to 63.2% failure probability for each type of specimens at a same calibration temperature by using the respective fracture toughness data. The method proceeds by obtaining the stress-strain curve of the material at the calibration temperature, generating finite element models for each type of specimens, and calculating the maximum principal stress and element volume of every element at K=K0 in each model. A series of values of m are assumed to compute a group of σu values for each type of specimens, and then m˜σu curves are plotted for each type of specimens. Brittle fracture assessment parameters are then determined for the material according to the coordinates of the intersection of the m˜σu curves.

Abstract translation: 基于Beremin模型的压力容器材料的脆性断裂评估参数的校准方法包括选择不同约束条件的至少两种类型的试样，并计算相同于每种类型试样的63.2％失效概率的断裂韧性值K0 通过使用相应的断裂韧度数据来校准温度。 该方法通过在校准温度下获得材料的应力 - 应变曲线，为每种类型的样品生成有限元模型，并计算每个模型中K = K0处的每个元素的最大主应力和元素体积。 假设m的一系列值计算每种类型样本的一组＆sgr u值，然后对于每种类型的样本绘制m〜＆sgr; u曲线。 然后根据m〜＆ugr曲线的交点坐标确定材料的脆性断裂评估参数。

公开(公告)号：US20140329718A1

公开(公告)日：2014-11-06

申请号：US14347575

申请日：2012-09-26

Applicant: East China University of Science and Technology

Inventor： Yi Yang ,  Jing Jin ,  Yuzheng Zhao ,  Qingxun Hu

IPC: G01N33/573 ,  G01N33/58 ,  G01N33/50

CPC classification number: G01N33/5735 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00 ,  C07K2319/60 ,  G01N33/5011 ,  G01N33/582 ,  G01N2500/02 ,  G01N2800/7028

Abstract: The invention relates to a genetically encoded fluorescent sensor for nicotinamide adenine dinucleotide, as well as methods of preparation and uses thereof. In one aspect, this invention relates to a sensor for detecting nicotinamide adenine dinucleotide, particularly, a recombinant fluorescent fusion protein sensor for detecting nicotinamide adenine dinucleotide. In one specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting reduced nicotinamide adenine dinucleotide (NADH); in another specific aspect, this invention relates to a recombinant fluorescent fusion protein sensor for detecting oxidized nicotinamide adenine dinucleotide (NAD+); in yet another aspect, the invention relates to a recombinant fluorescent fusion protein sensor for detecting the ratio of reduced to oxidized nicotinamide adenine dinucleotide. This invention also relates to the method of preparing the sensors, and uses of the sensors in detecting NADH, NAD+, NADH/NAD+ ratio, screening drugs and measuring NADH metabolism.

Abstract translation: 本发明涉及用于烟酰胺腺嘌呤二核苷酸的遗传编码荧光传感器，以及其制备方法和用途。 一方面，本发明涉及用于检测烟酰胺腺嘌呤二核苷酸的传感器，特别是用于检测烟酰胺腺嘌呤二核苷酸的重组荧光融合蛋白传感器。 在一个具体方面，本发明涉及用于检测还原型烟酰胺腺嘌呤二核苷酸（NADH）的重组荧光融合蛋白传感器。 在另一具体方面，本发明涉及用于检测氧化烟酰胺腺嘌呤二核苷酸（NAD +）的重组荧光融合蛋白传感器。 在另一方面，本发明涉及用于检测还原氧化烟酰胺腺嘌呤二核苷酸比例的重组荧光融合蛋白传感器。 本发明还涉及传感器的制备方法，以及传感器检测NADH，NAD +，NADH / NAD +比例，筛选药物和测定NADH代谢的用途。

公开(公告)号：US20140256875A1

公开(公告)日：2014-09-11

申请号：US14355534

申请日：2013-05-31

Applicant: Shanghai TianYang Hot Melt Adhesive Co., Ltd. ,  Kunhan TianYang hot Melt Adhesive Co., Ltd. ,  East China University of Science and Technology

Inventor： Zhelong Li ,  Wanyu Zhu ,  Zhiyan Ma ,  Zuoxiang Zeng ,  Tao Han

IPC: C09J167/02

CPC classification number: C09J167/02 ,  C08G63/183 ,  C08G63/78

Abstract: A method of preparing an amorphous, low-melting-point polyester hot-melt adhesive is disclosed. The method includes the steps of: 1) esterifying terephthalic acid, isophthalic acid and an aliphatic dicarboxylic acid with ethylene glycol and a C3-C6 dial in a presence of an esterification catalyst; 2) adding a stabilizer, a polycondensation catalyst and an antioxidant to a product from step 1) and lowering the pressure to perform a polycondensation reaction which results in a polyester having a melting point of lower than 120° C. The polyester hot-melt adhesive of the invention assumes a transparent state with no crystallization both in normal-temperature and high-temperature conditions and has a high flowability and an excellent wettability in low-temperature applications.

Abstract translation: 公开了一种制备无定形低熔点聚酯热熔粘合剂的方法。 该方法包括以下步骤：1）在酯化催化剂存在下，使对苯二甲酸，间苯二甲酸和脂族二羧酸与乙二醇和C3-C6表面酯化; 2）向步骤1）的产物中加入稳定剂，缩聚催化剂和抗氧化剂，降低压力进行缩聚反应，得到熔点低于120℃的聚酯。聚酯热熔胶 在常温和高温条件下呈现无结晶的透明状态，并且在低温应用中具有高流动性和优异的润湿性。

公开(公告)号：US20140127402A1

公开(公告)日：2014-05-08

申请号：US14152320

申请日：2014-01-10

Applicant: East China University of Science and Technology

Inventor： Yitao Long ,  Dawei Li ,  Lulu Qu ,  Wenlei Zhai ,  Jinqun Xue ,  Wei Song

IPC: G01N21/65

CPC classification number: G01N21/658 ,  B22F1/0018 ,  B22F1/0051 ,  B22F1/02 ,  B22F2999/00 ,  G01N21/554 ,  G01N33/18 ,  B22F2303/01 ,  B22F2301/255 ,  B22F2303/30 ,  B22F2302/256

Abstract: Provided herein are Raman active particles and methods for their preparation and use. The particles can include a SERS-active material that is at least partially encased within a spherical porous hollow casing. In some embodiments, this can be especially advantageous when employed for water analysis and/or being employed in combination with silica particles

Abstract translation: 本文提供的是拉曼活性颗粒及其制备和使用的方法。 颗粒可以包括至少部分地包裹在球形多孔中空壳体内的SERS-活性材料。 在一些实施方案中，当用于水分析和/或与二氧化硅颗粒组合使用时，这可能是特别有利的

公开(公告)号：US20140121349A1

公开(公告)日：2014-05-01

申请号：US14149611

申请日：2014-01-07

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Yongshu XIE

IPC: C08G79/00 ,  C07F3/06 ,  C07D213/36

CPC classification number: C08G79/00 ,  C07D213/36 ,  C07D213/74 ,  C07F3/003 ,  C07F3/06

Abstract: A hydrogel composition is formed by conveying separate first and second liquid components subject to a selectively applied application pressure P(A) into an outlet path for mixing and discharge. A liquid flushing agent is automatically conveyed into the outlet path subject to a substantially constantly applied purge pressure P(P) when the application of P(A) is interrupted, to continuously flush residual hydrogel composition from the outlet path.

Abstract translation: 通过将经过选择性施加的施加压力P（A）的分离的第一和第二液体组分输送到用于混合和排出的出口路径中来形成水凝胶组合物。 当中断P（A）的施加时，液体冲洗剂被自动地输送到出口路径中，经受基本上恒定地施加的清除压力P（P），以从出口路径连续冲洗剩余的水凝胶组合物。