公开(公告)号：US09677067B2

公开(公告)日：2017-06-13

申请号：US15154879

申请日：2016-05-13

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban Toro ,  Sebastian Treusch ,  Siyuan Chen ,  Cheng-Hsien Wu

IPC: C12N15/10 ,  C12N15/66 ,  C12P19/34

CPC classification number: C12N15/1031 ,  C12N15/1027 ,  C12N15/1093 ,  C12N15/66 ,  C12P19/34

Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.

公开(公告)号：US20160264958A1

公开(公告)日：2016-09-15

申请号：US15154879

申请日：2016-05-13

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Esteban Toro ,  Sebastian Treusch ,  Siyuan Chen ,  Cheng-Hsien Wu

IPC: C12N15/10

CPC classification number: C12N15/1031 ,  C12N15/1027 ,  C12N15/1093 ,  C12N15/66 ,  C12P19/34

Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.

Abstract translation: 提供了用于组装大核酸的方法和组合物，其中组装的大核酸在组装过程中缺少内部序列修饰。

公开(公告)号：US20160090592A1

公开(公告)日：2016-03-31

申请号：US14885962

申请日：2015-10-16

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE

IPC: C12N15/10

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20160089651A1

公开(公告)日：2016-03-31

申请号：US14885965

申请日：2015-10-16

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20150038373A1

公开(公告)日：2015-02-05

申请号：US14452429

申请日：2014-08-05

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: C12N15/10 ,  B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00623 ,  B01J2219/00637 ,  B01J2219/00709 ,  B01J2219/00722 ,  C12N15/09 ,  C12N15/1093 ,  C12N15/1096 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Abstract translation: 本文提供了从头合成的大型核酸文库，其错误率低。 此外，本文描述了用于制造高质量构建块（例如寡核苷酸）的装置。 可以使用微流控组件并行合成更长的核酸。 此外，本文的方法允许快速构建长，高质量基因的大型文库。 用于制造长质量和高质量核酸的大型文库的装置在本文中进一步描述。

公开(公告)号：US12091777B2

公开(公告)日：2024-09-17

申请号：US17030216

申请日：2020-09-23

Applicant: Twist Bioscience Corporation

Inventor： Ray Tabibiazar ,  Aaron Sato ,  Pankaj Garg ,  Qiang Liu

IPC: C40B40/10 ,  C07K16/28 ,  C12N15/10 ,  C40B40/08

CPC classification number: C40B40/10 ,  C07K16/2896 ,  C12N15/1044 ,  C40B40/08 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/92

Abstract: Provided herein are methods and compositions relating to prostaglandin D2 receptor 2 (DP2 or CRTH2R) libraries having nucleic acids encoding for a scaffold comprising a CRTH2R binding domain. CRTH2R libraries described herein encode for immunoglobulins including antibodies and single domain antibodies. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US20230331765A1

公开(公告)日：2023-10-19

申请号：US18319405

申请日：2023-05-17

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Pierre F. INDERMUHLE ,  Eugene P. MARSH ,  Andres FERNANDEZ ,  William BANYAI ,  Bill J. PECK

IPC: C07H21/04 ,  B01J19/00 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455

CPC classification number: C07H21/04 ,  B01J19/0046 ,  C07H1/00 ,  C12P19/34 ,  C23C16/455 ,  B01J2219/0059 ,  B01J2219/00527 ,  B01J2219/00596

Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.

公开(公告)号：US20230193383A1

公开(公告)日：2023-06-22

申请号：US18050912

申请日：2022-10-28

Applicant: Twist Bioscience Corporation

Inventor： Bill James PECK

IPC: C12Q1/6874 ,  B01J19/00 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722 ,  B01J2219/0061 ,  B01J2219/00632 ,  B01J2219/00518 ,  B01J2219/00637

Abstract: Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Additionally, devices described herein for de novo synthesis of oligonucleic acids encoding information related to the original source information may have a flexible material for oligonucleic acids extension.

公开(公告)号：US20230185971A1

公开(公告)日：2023-06-15

申请号：US17938234

申请日：2022-10-05

Applicant: Twist Bioscience Corporation

Inventor： Bill James PECK

IPC: G06F21/78 ,  C12N15/10

CPC classification number: G06F21/78 ,  C12N15/102 ,  C12N15/1089 ,  C12N15/1027 ,  G06F2212/402 ,  G06F2212/1052

Abstract: Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.

公开(公告)号：US20230175062A1

公开(公告)日：2023-06-08

申请号：US18062966

申请日：2022-12-07

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Jeremy LACKEY ,  Andres FERNANDEZ ,  David DODD ,  Alexander GORYAYNOV

IPC: C12Q1/6874 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2200/0652 ,  B01L2300/0819

Abstract: Provided herein are compositions, devices, systems and methods for single molecule sensing. Further provided are devices for nucleic acid sequencing. The compositions, devices, systems and methods described herein provide improved retrieval of biomolecule-based information.