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    AMYLOLYTIC ENZYME VARIANTS
    92.
    发明申请
    AMYLOLYTIC ENZYME VARIANTS 审中-公开

    公开(公告)号：US20100098804A1

    公开(公告)日：2010-04-22

    申请号：US12643104

    申请日：2009-12-21

    申请人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schäfer

    发明人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schäfer

    IPC分类号： A21D8/04 ,  C12N9/10 ,  C12P21/00

    CPC分类号： C12N9/2417 ,  A21D8/042

    摘要： The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

    摘要翻译： 发明人已经发现了一些引人注目的，并没有预先结合的Novamyl结构与报道的CGTase结构之间的结构相似性和差异，并且基于此，它们构建了具有CGT酶活性的麦芽糖α-淀粉酶变异体和具有麦芽糖α 淀粉酶活性。 此外，基于Novamyl和CGTases之间的序列同源性，发明人使用重组DNA方法构建了对母体酶的特定性质的一个或多个改进的杂合酶。

    Myceliophthora thermophila laccase variants
    95.
    发明授权
    Myceliophthora thermophila laccase variants 失效
    肉毒杆菌嗜热链霉菌漆酶变体

    公开(公告)号：US07622287B2

    公开(公告)日：2009-11-24

    申请号：US09732350

    申请日：2000-12-07

    申请人： Allan Svendsen ,  Feng Xu

    发明人： Allan Svendsen ,  Feng Xu

    IPC分类号： C12N9/02 ,  C12Q1/00 ,  C12Q1/68 ,  C12P21/04 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12Y110/03002 ,  C11D3/38636 ,  C12N9/0061

    摘要： The present invention relates to a method of designing laccase mutants with increased oxidation potential and/or changed pH optimum and/or altered mediator pathway and/or altered O2/OH−-pathway, which method is based on the hitherto unknown three dimensional structure of laccases, wherein the parent laccase is a Myceliophthora thermophila, Polyporus pinsitus, or Coprinus cinereus laccase.

    摘要翻译： 本发明涉及一种设计具有增加的氧化电位和/或改变的pH最佳和/或改变的介体途径和/或改变的O 2 / OH途径的漆酶突变体的方法，该方法基于迄今为止未知的三维结构 漆酶，其中母漆酶是嗜热丝孢杆菌，多孔菌或灰霉菌漆酶。

    α-amylase variants
    96.
    发明授权
    α-amylase variants 有权
    α-淀粉酶变体

    公开(公告)号：US07601527B2

    公开(公告)日：2009-10-13

    申请号：US11039565

    申请日：2005-01-19

    申请人： Allan Svendsen ,  Sóeren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

    发明人： Allan Svendsen ,  Sóeren Kjaerulff ,  Henrik Bisgaard-Frantzen ,  Carsten Andersen

    IPC分类号： C12N9/28

    CPC分类号： C12N9/2414 ,  C07H21/04 ,  C11D3/38609 ,  C11D3/38618 ,  C12N9/2417

    摘要： The invention relates to a variant of a parent Termamyl-like α-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an α-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an α-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an α-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an α-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like α-amylase, which variant exhibits increased.

    摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其包含2,3,4,5或6个/位置的突变。 这些变体在高温下（相对于亲本）具有增加的稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体表现出增加。

    SUBTILASES
    98.
    发明申请
    SUBTILASES 失效

    公开(公告)号：US20090203111A1

    公开(公告)日：2009-08-13

    申请号：US12401077

    申请日：2009-03-10

    申请人： Allan Svendsen ,  Stefan Minning

    发明人： Allan Svendsen ,  Stefan Minning

    IPC分类号： C12N9/54

    CPC分类号： C12N9/54 ,  C07K2299/00

    摘要： The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN′ subtilase and to JP170 and BPN′ variants having altered properties as compared to the parent JP170/BPN′ subtilase.

    摘要翻译： 本发明涉及与亲本JP170 / BPN'脱羧酶相比，生产亲本JP170脱羧酶和亲本BPN'脱羧酶的变体的方法和具有改变的性质的JP170和BPN'变体的方法。

    Amylolytic Enzyme Variants
    99.
    发明申请
    Amylolytic Enzyme Variants 审中-公开
    淀粉分解酶变体

    公开(公告)号：US20090142803A1

    公开(公告)日：2009-06-04

    申请号：US12208425

    申请日：2008-09-11

    申请人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Denmark Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schafer

    发明人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Denmark Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schafer

    IPC分类号： C12N9/10 ,  C12P21/00

    CPC分类号： C12N9/2417 ,  A21D8/042

    摘要： The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

    摘要翻译： 发明人已经发现了一些引人注目的，并没有预先结合的Novamyl结构与报道的CGTase结构之间的结构相似性和差异，并且基于此，它们构建了具有CGT酶活性的麦芽糖α-淀粉酶变异体和具有麦芽糖α 淀粉酶活性。 此外，基于Novamyl和CGTases之间的序列同源性，本发明人使用重组DNA方法构建了对母体酶的特定性质具有一个或多个改进的杂合酶。