公开(公告)号：US08262900B2

公开(公告)日：2012-09-11

申请号：US12002781

申请日：2007-12-17

申请人： Jonathan M. Rothberg ,  Wolfgang Hinz

发明人： Jonathan M. Rothberg ,  Wolfgang Hinz

IPC分类号： C12Q1/25 ,  G01N27/414

CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US20120061256A1

公开(公告)日：2012-03-15

申请号：US13229439

申请日：2011-09-09

申请人： Jonathan M. Rothberg ,  Wolfgang Hinz

发明人： Jonathan M. Rothberg ,  Wolfgang Hinz

IPC分类号： G01N27/414 ,  G01N33/50

CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US20110318820A1

公开(公告)日：2011-12-29

申请号：US13172048

申请日：2011-06-29

申请人： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James Ball

发明人： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James Ball

IPC分类号： C12M1/34

CPC分类号： C12Q1/6874 ,  G01N27/4145

摘要： The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

摘要翻译： 本公开涉及新型颗粒组合物和制备所述组合物在核酸分析中的应用的方法，以及用于其的装置和系统。

公开(公告)号：US20110301041A1

公开(公告)日：2011-12-08

申请号：US13036526

申请日：2011-02-28

申请人： John DAVIDSON ,  Wolfgang Hinz ,  Jonathan M. Rothberg ,  Richard Whitaker

发明人： John DAVIDSON ,  Wolfgang Hinz ,  Jonathan M. Rothberg ,  Richard Whitaker

IPC分类号： C40B20/00 ,  C12Q1/68

CPC分类号： C12Q1/6869 ,  C12N9/1252 ,  C12Q2563/113 ,  C12Q2563/116

摘要： Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

公开(公告)号：US20110262903A1

公开(公告)日：2011-10-27

申请号：US13035081

申请日：2011-02-25

申请人： John DAVIDSON ,  Wolfgang Hinz ,  Jonathan M. Rothberg

发明人： John DAVIDSON ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： C12Q1/68

CPC分类号： C12N9/1252

摘要： Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

公开(公告)号：US20110251110A1

公开(公告)日：2011-10-13

申请号：US13044352

申请日：2011-03-09

申请人： Jonathan M. Rothberg ,  John H. Leamon ,  John F. Davidson ,  Antoine M. Van Oijen ,  Wolfgang Hinz ,  Melville Davey ,  Bradley Hann ,  Jonathan Schultz

发明人： Jonathan M. Rothberg ,  John H. Leamon ,  John F. Davidson ,  Antoine M. Van Oijen ,  Wolfgang Hinz ,  Melville Davey ,  Bradley Hann ,  Jonathan Schultz

IPC分类号： C40B60/12 ,  C12M1/40

CPC分类号： C12Q1/6874 ,  C12Q2523/303 ,  C12Q2565/131 ,  C12Q2525/204 ,  C12Q2521/101 ,  C12Q2535/122

摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.

公开(公告)号：US09534092B2

公开(公告)日：2017-01-03

申请号：US13371264

申请日：2012-02-10

申请人： Wolfgang Hinz ,  Ryan Jones ,  David Light

发明人： Wolfgang Hinz ,  Ryan Jones ,  David Light

IPC分类号： B07B1/00 ,  C08J3/12 ,  C12N15/10

CPC分类号： C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

摘要： A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

摘要翻译： 制备水凝胶颗粒的方法包括将包含多个水凝胶颗粒的溶液施加到搅拌槽。 过滤器的滞留侧限定了搅拌槽的下表面。 过滤器具有滞留侧和渗透侧。 该方法还包括在搅拌槽内搅拌溶液的同时，以第一流速向缓冲池分配缓冲溶液，并以第二流量使用泵从过滤器的渗透侧抽出渗透物，渗透物包括 多个水凝胶颗粒的一个子集。

公开(公告)号：US08492799B2

公开(公告)日：2013-07-23

申请号：US13149250

申请日：2011-05-31

申请人： Jonathan M Rothberg ,  Wolfgang Hinz

发明人： Jonathan M Rothberg ,  Wolfgang Hinz

IPC分类号： G01N27/403

CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US20120085660A1

公开(公告)日：2012-04-12

申请号：US13245721

申请日：2011-09-26

申请人： Jonathan M. Rothberg ,  Wolfgang Hinz

发明人： Jonathan M. Rothberg ,  Wolfgang Hinz

IPC分类号： G01N27/26

CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

摘要翻译： 提供了与用于分析物检测和测量的大规模FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。

公开(公告)号：US20120061733A1

公开(公告)日：2012-03-15

申请号：US13149250

申请日：2011-05-31

申请人： Jonathan M. Rothberg ,  Wolfgang Hinz

发明人： Jonathan M. Rothberg ,  Wolfgang Hinz

IPC分类号： H01L29/788 ,  H01L21/336

CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

摘要： Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.