公开(公告)号：US20220011200A1

公开(公告)日：2022-01-13

申请号：US16924204

申请日：2020-07-09

申请人： Yan Wang ,  Raymond Yang

发明人： Yan Wang ,  Raymond Yang

IPC分类号： G01N1/28 ,  C12Q1/6806 ,  C12Q1/686

摘要： Disclosed are methods and devices for loading a liquid sample into an array substrate with a plurality of compartments, for example, a dPCR chip. The method uses a loading device having a single flat surface to efficiently load a liquid sample into a plurality of compartments in the array substrate.

公开(公告)号：US20210381980A1

公开(公告)日：2021-12-09

申请号：US16894612

申请日：2020-06-05

申请人： Yan Wang ,  Cory McCluskey

发明人： Yan Wang ,  Cory McCluskey

IPC分类号： G01N21/64 ,  B01L9/00 ,  C12Q1/686

摘要： Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.

公开(公告)号：US10230174B2

公开(公告)日：2019-03-12

申请号：US15239347

申请日：2016-08-17

申请人： Yan Wang

发明人： Yan Wang

IPC分类号： H01Q21/24 ,  H01Q1/48 ,  H01Q9/04 ,  H01Q21/06 ,  H01Q21/00 ,  H01Q3/30

摘要： A frequency diverse phased-array antenna operates simultaneously in two bands. A checkerboard of antenna elements for a first wavelength is offset with a second checkerboard of second antenna elements. Within the substrate is a three dimensional checkerboard of ground planes and ground walls which provide signal isolation between the bands. Multiple ground planes optimize operation at the several frequencies. Phased-array elements are isolated by a conductive wall in a multi-layer substrate. Orthogonal polarization of antenna patches further improve signal discrimination. Below the surface layer, another conductive wall isolates each quadrature hybrid. The conductive wall can be realized by metal vias or metal mesh infused through a dielectric and surrounding a raised ground plane to isolate electrical fields at each frequency. A conductive wall also provides quadrature hybrid isolation.

公开(公告)号：US10093916B2

公开(公告)日：2018-10-09

申请号：US15159345

申请日：2016-05-19

申请人： Kenneth S. Manning ,  Yan Wang ,  Dustin Nielsen ,  Ryan Ruf ,  Colin Crowley ,  Jon Restivo ,  Dana Spangenberg ,  Karla Anhalt ,  Mark Romich ,  Anisha Akula ,  Carmen Fritz

发明人： Kenneth S. Manning ,  Yan Wang ,  Dustin Nielsen ,  Ryan Ruf ,  Colin Crowley ,  Jon Restivo ,  Dana Spangenberg ,  Karla Anhalt ,  Mark Romich ,  Anisha Akula ,  Carmen Fritz

IPC分类号： C12N9/00 ,  C12N9/94 ,  A61K38/46 ,  C12N7/00 ,  A61K9/00 ,  A61K35/39 ,  A61K31/327

摘要： A method for the reduction or inactivation of viral and microbial content in the manufacturing of pancreatin API is disclosed. The method includes treating animal-derived tissue with peracetic acid to reduce viral activity and bacterial load prior to processing. In particular, the method includes treating porcine pancreas glands with peracteic acid prior to extracting a pancreatin API from the treated glandular tissue.

公开(公告)号：US20180258465A1

公开(公告)日：2018-09-13

申请号：US15901900

申请日：2018-02-22

申请人： Yan Wang

发明人： Yan Wang

IPC分类号： C12Q1/686 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/686 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2300/1827 ,  C12Q2537/143 ,  C12Q2561/113 ,  C12Q2563/159 ,  C12Q2565/629

摘要： Disclosed are devices that can perform multiple independent digital PCRs with real time monitoring capability. The device comprises multiple PCR mini-reactors thermally coupled with its own temperature control element, a detection unit, and a motorized platform for holding and moving the PCR mini-reactors. The real time digital PCR device can simultaneously perform multiple digital PCRs, generate amplification curves of thousands and millions of individual PCR processes, evaluate binary readouts based on the amplification curves, and identify different target sequences based on the amplification curves. Methods of using the real time digital PCR device to detect target nucleic acid and count circulating tumor cells are also disclosed.

公开(公告)号：US09768460B2

公开(公告)日：2017-09-19

申请号：US13994906

申请日：2011-12-16

申请人： Andrew Gabriel Rinzler ,  Rajib Kumar Das ,  Yan Wang ,  Hai-Ping Cheng

发明人： Andrew Gabriel Rinzler ,  Rajib Kumar Das ,  Yan Wang ,  Hai-Ping Cheng

IPC分类号： H01M8/06 ,  H01M8/0656 ,  H01M4/90 ,  H01M4/96 ,  H01M8/0234 ,  H01M8/0239 ,  H01M8/18 ,  C25B11/12 ,  H01M4/86 ,  H01M4/88

CPC分类号： H01M8/0656 ,  C25B11/12 ,  H01M4/8615 ,  H01M4/8825 ,  H01M4/90 ,  H01M4/96 ,  H01M8/0234 ,  H01M8/0239 ,  H01M8/186 ,  H01M2250/10 ,  H01M2250/402 ,  Y02B90/12 ,  Y02B90/14 ,  Y02E60/528 ,  Y02P20/133

摘要： An electrode comprises an acid treated, cathodically cycled carbon-comprising film or body. The carbon consists of single walled nanotubes (SWNTs), pyrolytic graphite, microcrystalline graphitic, any carbon that consists of more than 99% sp2 hybridized carbons, or any combination thereof. The electrode can be used in an electrochemical device functioning as an electrolyzer for evolution of hydrogen or as a fuel cell for oxidation of hydrogen. The electrochemical device can be coupled as a secondary energy generator into a system with a primary energy generator that naturally undergoes generation fluctuations. During periods of high energy output, the primary source can power the electrochemical device to store energy as hydrogen, which can be consumed to generate electricity as the secondary source during low energy output by the primary source. Solar cells, wind turbines and water turbines can act as the primary energy source.

公开(公告)号：US09622095B2

公开(公告)日：2017-04-11

申请号：US13382521

申请日：2010-07-09

申请人： Weiguo Ma ,  Bing Liu ,  Jiye Tang ,  Yan Wang

发明人： Weiguo Ma ,  Bing Liu ,  Jiye Tang ,  Yan Wang

IPC分类号： H04W76/02 ,  H04W24/04 ,  H04W88/08 ,  H04W92/12

CPC分类号： H04W24/04 ,  H04W76/10 ,  H04W88/08 ,  H04W92/12

摘要： A method and a device for maintaining a Long Term Evolution (LTE) base station are provided by the embodiments of the present invention. Depending on the LTE access network system and air interface characteristics, by making a few processing flow changes in such mode or method, a wireless communication link is established between a network maintenance device and a base station to implement the base station maintenance and data communication. Application of the technical solutions provided by the embodiments of the present invention can implement the base station maintenance and data communication by the wireless mode, reduce the cost of the network maintenance and prevent the destruction and influence of human factors on the operation and maintenance of the base station.

公开(公告)号：US09598527B2

公开(公告)日：2017-03-21

申请号：US11638876

申请日：2006-12-14

申请人： Thomas G. Rukavina ,  Robert Hunia ,  Yan Wang

发明人： Thomas G. Rukavina ,  Robert Hunia ,  Yan Wang

IPC分类号： C08G18/32 ,  C08G18/42 ,  C08G18/44 ,  C08G18/73 ,  C08G18/75 ,  C08G18/76 ,  C08G18/79 ,  C09D175/04 ,  B32B27/40 ,  B32B9/00 ,  B32B27/12 ,  B32B27/10 ,  B32B27/08 ,  B32B21/08 ,  B32B15/08 ,  B32B9/04 ,  B32B11/04 ,  B32B13/12

CPC分类号： C08G18/3206 ,  B32B9/005 ,  B32B9/045 ,  B32B11/046 ,  B32B13/12 ,  B32B15/08 ,  B32B21/08 ,  B32B27/08 ,  B32B27/10 ,  B32B27/12 ,  B32B27/40 ,  B32B2307/302 ,  B32B2307/412 ,  B32B2307/54 ,  B32B2307/554 ,  B32B2307/558 ,  B32B2307/712 ,  B32B2307/718 ,  B32B2551/00 ,  B32B2571/02 ,  C08G18/3203 ,  C08G18/3212 ,  C08G18/3221 ,  C08G18/4277 ,  C08G18/44 ,  C08G18/73 ,  C08G18/758 ,  C08G18/765 ,  C08G18/7671 ,  C08G18/792 ,  C08G18/798 ,  C09D175/04 ,  Y10T428/249924 ,  Y10T428/31551 ,  Y10T428/31591 ,  Y10T428/31601

摘要： The present invention provides polyurethanes including a reaction product of components including: (a) about 1 equivalent of at least one polyisocyanate; (b) about 0.05 to about 0.9 equivalents of at least one branched polyol having 4 to 18 carbon atoms and at least 3 hydroxyl groups; and (c) about 0.1 to about 0.95 equivalents of at least one diol having 2 to 18 carbon atoms, wherein the reaction product components are essentially free of polyester polyol and polyether polyol and the reaction components are maintained at a temperature of at least about 100° C. for at least about 10 minutes, compositions, coatings and articles made therefrom and methods of making the same.

公开(公告)号：US09493824B2

公开(公告)日：2016-11-15

申请号：US13328154

申请日：2011-12-16

申请人： Yan Wang

发明人： Yan Wang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/107

摘要： Universal reference dye mixtures for quantitative amplification, and uses thereof, are provided.

摘要翻译： 提供用于定量扩增的通用参考染料混合物及其用途。

公开(公告)号：US20160201131A1

公开(公告)日：2016-07-14

申请号：US14879099

申请日：2015-10-09

申请人： Yan Wang ,  Jianming Xu ,  Chun Gong

发明人： Yan Wang ,  Jianming Xu ,  Chun Gong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6809 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2535/122 ,  C12Q2537/165

摘要： The present invention provides a method for detecting low frequency drug resistant mutations using ultra deep amplicon sequencing. Multiple selection criteria based on biological relevance were applied to filter out low frequency background noise and lead to reliable identification of drug resistance related mutations. A method to predict the occurrence of drug resistance during the treatment is also provided.

摘要翻译： 本发明提供使用超深度扩增子测序检测低频耐药性突变的方法。 应用基于生物相关性的多种选择标准来筛选低频背景噪声，并导致耐药相关突变的可靠鉴定。 还提供了一种预测治疗期间耐药性发生的方法。