Capacitance Detection in a Droplet Actuator
    91.
    发明申请
    Capacitance Detection in a Droplet Actuator 有权
    滴液致动器中的电容检测

    公开(公告)号:US20100194408A1

    公开(公告)日:2010-08-05

    申请号:US12527208

    申请日:2008-02-15

    IPC分类号: G01R27/26

    摘要: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

    摘要翻译: 一种用于检测液滴致动器上电容的方法,电路和装置,特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在,部分存在或不存在:(a)提供液滴致动器,其包括:(i) 基板,其包括布置在所述基板上的电极,用于在所述基板的表面上进行液滴操作; (ii)电容检测电路,用于检测一个或多个电极处的液滴操作表面处的电容; (b)在一个或多个电极处检测液滴操作表面处的电容; 和(c)根据电容确定在电极处的液滴操作表面处的液滴的存在,部分存在或不存在。

    Droplet Actuator Structures
    93.
    发明申请
    Droplet Actuator Structures 审中-公开
    液滴致动器结构

    公开(公告)号:US20100025250A1

    公开(公告)日:2010-02-04

    申请号:US12529041

    申请日:2008-03-03

    IPC分类号: B41J2/045 B03C9/00

    摘要: The objective of this research is to model and design a microfluidic system that uses electrostatic fields to induce movement of discrete droplets of solution. Of particular interest is movement of droplets of H2O for use in biological testing with lab-on a-chip and μTAS systems. Using computer modeling, the electric-fields for planar electrode configurations positioned on an insulating substrate are calculated for a hemispherical drop of H2O on the substrate at various positions. From these electric-fields the force on the drop is calculated. These models show that electrostatic actuation of droplets of H2O is possible. However, as the complexity of the model increases the properties of the system become less desirable and actuation may not be possible. Using microfabrication techniques, the modeled microfluidic systems have been built for testing using a Kapton substrate with copper electrodes. Hexadecenyltrichlorosilane (HTS), a self-assembled monolayer, and its oxidant have been studied and found capable of providing hydrophobic and hydrophilic surface coatings for the systems.

    摘要翻译: 本研究的目的是建立和设计一个使用静电场诱导离散液滴移动的微流体系统。 特别令人感兴趣的是使用实验室上的芯片和muTAS系统用于生物测试的H 2 O滴。 使用计算机建模,计算位于绝缘基板上的平面电极配置的电场,用于在不同位置的衬底上的半球形H 2 O的下降。 从这些电场计算出液滴上的力。 这些模型显示H2O的液滴的静电驱动是可能的。 然而,随着模型的复杂性增加,系统的性能变得不太可取,并且致动可能是不可能的。 使用微细加工技术,已经建立了用于使用具有铜电极的Kapton衬底进行测试的建模的微流体系统。 已经研究了十六碳烯三氯硅烷(HTS),一种自组装单层及其氧化剂,能够为系统提供疏水和亲水的表面涂层。

    Manipulation of Cells on a Droplet Actuator
    97.
    发明申请
    Manipulation of Cells on a Droplet Actuator 有权
    在液滴致动器上操纵细胞

    公开(公告)号:US20090155902A1

    公开(公告)日:2009-06-18

    申请号:US12334575

    申请日:2008-12-15

    IPC分类号: C12N5/08

    摘要: A method of inoculating a culture medium including providing a droplet including a single cell type on a droplet actuator and inoculating a culture medium with the droplet. A method of providing a metabolically useful substance to a cell culture, including providing a droplet actuator including a cell culture droplet loaded thereon, the sample droplet including cells and a cell culture medium, and a second droplet comprising a metabolically useful substance. The method also includes conducting one or more droplet operations to combine the cell culture droplet with the second droplet on the droplet actuator. Related methods, droplet actuators, and systems are also provided.

    摘要翻译: 一种接种培养基的方法,包括在液滴致动器上提供包括单细胞类型的液滴并用液滴接种培养基。 提供代谢有用的物质给细胞培养物的方法,包括提供包括其上装载有细胞培养物液滴的液滴致动器,所述样品液滴包括细胞和细胞培养基,以及包含代谢有用物质的第二液滴。 该方法还包括进行一个或多个液滴操作以将细胞培养液滴与液滴致动器上的第二液滴组合。 还提供了相关方法,液滴致动器和系统。