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    Methods for making metallopeptides and metallopeptide libraries
    92.
    发明申请
    Methods for making metallopeptides and metallopeptide libraries 审中-公开
    制备金属肽和金属肽文库的方法

    公开(公告)号：US20060040324A1

    公开(公告)日：2006-02-23

    申请号：US11198510

    申请日：2005-08-05

    申请人： Shubh Sharma

    发明人： Shubh Sharma

    IPC分类号： C40B50/14 ,  C40B50/06

    CPC分类号： C40B30/04 ,  A61K51/0497 ,  A61K51/08 ,  A61K51/082 ,  A61K51/083 ,  A61K51/086 ,  A61K51/088 ,  A61K2121/00 ,  A61K2123/00 ,  C40B40/16 ,  C40B50/06 ,  C40B50/14

    摘要： A method of making metallopeptide libraries and combinational libraries. The metallopeptide library members name a conformationally constrained global secondary structure obtained upon complexing with a metal ion. The metallopeptides are of the general formula: R1—X—R2 where X is a plurality of amino acids and includes a complexing backbone for complexing metal ions, so that substantially all of the valences of the metal ion are satisfied upon complexation of the metal ion with X, resulting in a specific regional secondary structure forming a part of the global secondary structure; and where R1 and R2 each include from 0 to about 20 amino acids, the amino acids being selected so that upon complexing the metal ion with X at least a portion of either R1 or R2 or both have a structure forming the balance of the conformationally constrained global secondary structure.

    摘要翻译： 制备金属肽文库和组合文库的方法。 金属肽文库成员命名为与金属离子络合时获得的构象受限的全局二级结构。 金属肽具有以下通式：<？in-line-formula description =“In-line Formulas”end =“lead”？> R＆lt; 1＆gt; -XR 2＆lt; ？在线公式描述=“在线公式”end =“tail”？>其中X是多个氨基酸，并且包括用于络合金属离子的络合主链，使得金属离子的基本上全部的价态 对金属离子与X的络合是令人满意的，导致特定的区域二级结构形成全球二级结构的一部分; 并且其中R 1和R 2各自包含0至约20个氨基酸，所述氨基酸被选择为使得当将金属离子与X的至少一部分 或者R 1或R 2或两者具有形成构象约束的全局二级结构的平衡的结构。

    Recombinant peptides derived from the Mc3 anti-BA46 antibody, methods of use thereof, and methods of humanizing antibody peptides
    94.
    发明申请
    Recombinant peptides derived from the Mc3 anti-BA46 antibody, methods of use thereof, and methods of humanizing antibody peptides 失效
    衍生自Mc3抗BA46抗体的重组肽，其使用方法和抗体肽人源化方法

    公开(公告)号：US20050169915A1

    公开(公告)日：2005-08-04

    申请号：US10965616

    申请日：2004-10-13

    申请人： Fernando Do Couto ,  Roberto Ceriani ,  Jerry Peterson

    发明人： Fernando Do Couto ,  Roberto Ceriani ,  Jerry Peterson

    IPC分类号： A61K38/00 ,  A61K51/10 ,  C07K16/30 ,  A61K39/395 ,  C07K16/44

    CPC分类号： A61K51/1051 ,  A61K38/00 ,  A61K51/1081 ,  A61K51/1093 ,  A61K51/1096 ,  A61K2039/505 ,  A61K2121/00 ,  A61K2123/00 ,  C07K16/3015 ,  C07K2317/24 ,  C07K2317/56

    摘要： The present invention provides recombinant peptides that specifically and selectively bind to the human milk fat globule (HMFG) antigen, BA46. In particular, the present invention provides recombinant variants of the Mc3 antibody, including humanized versions of Mc3. The variant Mc3 peptides are particularly useful for diagnostic, prognostic, and therapeutic applications in the field of breast cancer. The present invention also provides methods for the humanization of antibodies such as murine monoclonal antibodies. The novel humanization methods are applied to the production of humanized Mc3 antibodies and it is shown that these humanized antibodies retain the ability to engage in high affinity binding to their cognate antigen. Such humanization enables the use of these antibodies for immunodiagnostic and immunotherapeutic applications in humans.

    摘要翻译： 本发明提供了特异性且选择性地结合人乳脂肪球（HMFG）抗原BA46的重组肽。 特别地，本发明提供Mc3抗体的重组变体，包括人源化形式的Mc3。 该变体Mc3肽特别可用于乳腺癌领域的诊断，预后和治疗应用。 本发明还提供了抗体如人单克隆抗体人源化的方法。 新型人源化方法适用于人源化Mc3抗体的产生，并且显示这些人源化抗体保留参与与其同源抗原的高亲和力结合的能力。 这样的人源化使得能够使用这些抗体用于人体免疫诊断和免疫治疗应用。

    Method for producing a recombinant polypeptide
    98.
    发明申请
    Method for producing a recombinant polypeptide 审中-公开
    生产重组多肽的方法

    公开(公告)号：US20050084969A1

    公开(公告)日：2005-04-21

    申请号：US10497015

    申请日：2002-11-26

    申请人： Kurt Schorgendorfer ,  Jorg Windisch ,  Renate Kunert ,  Florian Unterluggauer

    发明人： Kurt Schorgendorfer ,  Jorg Windisch ,  Renate Kunert ,  Florian Unterluggauer

    IPC分类号： C12N15/09 ,  A61K38/22 ,  A61P7/00 ,  A61P7/06 ,  C07K14/505 ,  C12N5/10 ,  C12N15/85 ,  C12N15/90 ,  C12P21/02 ,  C12N5/06

    CPC分类号： C07K14/505 ,  A61K2121/00 ,  C12N15/85 ,  C12N15/90 ,  C12N15/907 ,  C12N2840/203 ,  C12P21/02

    摘要： The invention relates to a method for producing a transformed eukaryotic host cell which expresses a recombinant polypeptide of interest which method comprises introducing into a eukaryotic host cell: (a) a first polynucleotide vector which comprises (i) a first nucleotide sequence which encodes a recombinant polypeptide of interest, and (ii) a second nucleotide sequence encoding a selectable marker, which second nucleotide sequence is amplified when the host cell is contacted with a selection agent; and (b) a second polynucleotide vector having essentially the same nucleotide sequence as the first polynucleotide vector except that the second nucleotide sequence is replaced with a third nucleotide sequence which encodes a different selectable marker; the first polynucleotide vector and second polynucleotide vector being stably integrated into the genome of the host cell.

    摘要翻译： 本发明涉及用于产生表达重组感兴趣的多肽的转化真核宿主细胞的方法，所述方法包括将真核宿主细胞导入：（a）第一多核苷酸载体，其包含（i）第一核苷酸序列，其编码重组 多肽，和（ii）编码选择性标记的第二核苷酸序列，当宿主细胞与选择剂接触时，第二核苷酸序列被扩增; 和（b）第二多核苷酸载体，其具有与第一多核苷酸载体基本上相同的核苷酸序列，除了第二核苷酸序列被编码不同选择标记的第三核苷酸序列替代; 第一多核苷酸载体和第二多核苷酸载体稳定整合到宿主细胞的基因组中。