公开(公告)号：US5155166A

公开(公告)日：1992-10-13

申请号：US539680

申请日：1990-06-18

申请人： Susan J. Danielson ,  Donald P. Specht

发明人： Susan J. Danielson ,  Donald P. Specht

IPC分类号： C08F8/32 ,  C12N11/08 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547

CPC分类号： C12N11/08 ,  G01N33/54353 ,  G01N33/545 ,  Y10S530/812 ,  Y10S530/815 ,  Y10S530/816

摘要： Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, including enzymes, polypeptides and proteins. This attachment is carried out using specific carbamoylonium compounds, namely certain 1-(1-pyrrolidinylcarbonyl)pyridinium salts. These compounds react with the carboxyl groups on the particles to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a 1-(1-pyrrolidinylcarbonyl)pyridinium salt.

公开(公告)号：US5132226A

公开(公告)日：1992-07-21

申请号：US451708

申请日：1989-12-18

申请人： Michael Dreher ,  Heinz Hirsch

发明人： Michael Dreher ,  Heinz Hirsch

IPC分类号： G01N31/00 ,  C12Q1/28 ,  G01N21/78 ,  G01N33/52 ,  G01N33/535 ,  G01N33/547

CPC分类号： G01N33/52 ,  Y10T436/145555 ,  Y10T436/17

摘要： A method and an agent for the determination of maleimide groups, comprising reacting a sample containing maleimide groups with a reagent containing thiol groups and then with a chromogen or fluorogen which reacts with thiol groups, and measuring the change in intensity.

摘要翻译： 一种用于测定马来酰亚胺基团的方法和试剂，包括使含有马来酰亚胺基团的样品与含有硫醇基团的试剂反应，然后与与硫醇基团反应的色原体或氟原子反应，并测量强度变化。

公开(公告)号：US5108923A

公开(公告)日：1992-04-28

申请号：US34801

申请日：1987-04-03

申请人： Christine V. Benedict ,  Paul T. Picciano

发明人： Christine V. Benedict ,  Paul T. Picciano

IPC分类号： C12M3/00 ,  A61L27/38 ,  C07K2/00 ,  C07K14/00 ,  C12N5/00 ,  C12N5/06 ,  C12N11/00 ,  C12N11/08 ,  G01N33/547

CPC分类号： C12N5/0068 ,  A61L27/38 ,  C12N2533/50

摘要： A cell culturing system, methods for the preparation thereof, and methods for affixing other biologically active moieties to a substrate are provided. Said cell culturing system comprises: a substrate; a coating thereon of a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR1##wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; viable cells affixed to said coated substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.

摘要翻译： 提供了一种细胞培养系统，其制备方法和将其它生物活性部分固定在基底上的方法。 所述细胞培养系统包括：基质; 其中包含含有约35至100重量％的具有重复十肽单元的纯生物粘附多酚蛋白质的多酚蛋白的无菌制剂的涂层：其中N是整数范围 约10至约100，其中每个X独立地选自羟基和氢，并且其中每个R独立地选自氢和甲基; 固定在所述涂覆的基底上的活细胞; 和与所述细胞接触的营养介质，由此所述细胞执行正常的代谢细胞功能。

公开(公告)号：US4829101A

公开(公告)日：1989-05-09

申请号：US75416

申请日：1987-07-20

申请人： Dieter Kraemer ,  Werner Siol ,  Gerhard Markert ,  Norbert Suetterlin ,  Cornelia Feil

发明人： Dieter Kraemer ,  Werner Siol ,  Gerhard Markert ,  Norbert Suetterlin ,  Cornelia Feil

IPC分类号： C08F2/00 ,  A61K39/00 ,  C08F2/22 ,  C08F291/00 ,  C08J3/05 ,  C08L33/00 ,  C08L33/02 ,  C08L33/24 ,  C08L33/26 ,  C08L51/00 ,  C08L51/02 ,  C08L57/00 ,  C08L101/00 ,  C12N11/02 ,  G01N33/535 ,  G01N33/543 ,  G01N33/545 ,  G01N33/546 ,  G01N33/547

CPC分类号： G01N33/54313 ,  C08F291/00 ,  C12N11/02 ,  Y10S525/902

摘要： Polymer particles dispersible to form a latex and latices of such polymer particles, said particles being adaptable to the fixing or bonding thereto of a biologically active substance and having a core-shell construction wherein the polymer material of the core determines the stability of the form of the latex particles and their redispersibility, and the material of the shell(1) is so hydrophilic that it would be completely or largely water soluble if it were not anchored to the core material and/or crosslinked,(2) contains functional groups which are suitable for the covalent fixation or bonding of biological active substances, and(3) in an anhydrous condition has a T.sub..lambda.max from 20.degree.-250.degree. C., depending on its composition, and methods for bonding a biologically active substance to such particles, for example to prepare a diagnostic reagent.

摘要翻译： 可分散以形成胶乳的聚合物颗粒和这种聚合物颗粒的胶乳，所述颗粒适于固定或粘合生物活性物质并具有核 - 壳结构，其中芯的聚合物材料决定了 胶乳颗粒及其再分散性，并且壳（1）的材料是亲水性的，如果它不被锚定到芯材料和/或交联的话，它将完全或大部分是水溶性的，（2）包含官能团 适用于生物活性物质的共价固定或结合，（3）在无水状态下，根据其组成，T值为20〜250℃，以及将生物活性物质与该颗粒结合的方法 ，例如制备诊断试剂。

公开(公告)号：US4826760A

公开(公告)日：1989-05-02

申请号：US922243

申请日：1986-10-23

申请人： James R. Privitera

发明人： James R. Privitera

IPC分类号： G01N33/569 ,  G01N33/546 ,  G01N33/547 ,  G01N33/577

CPC分类号： G01N33/56972 ,  Y10T436/25375

摘要： An improved method for determining a helper/suppressor ratio of lysed whole blood. The process includes the steps of high speed microcentrifuging the lysed whole blood, decanting, washing and resuspending the sample followed by a second high speed microcentrifuging step. The supernatant fluid is poured off and a tagging solution containing colored beads which bind to T4 and T8 lymphocytes is added followed by low speed microcentrifuging, incubating and counting under a microscope.

摘要翻译： 一种用于确定裂解全血的辅助/抑制比的改进方法。 该方法包括以下步骤：将裂解的全血高速微量离心，倾析，洗涤和重新悬浮样品，然后进行第二次高速微量离心步骤。 倒出上清液，加入含有与T4和T8淋巴细胞结合的着色珠粒的标记溶液，然后低速微量离心，在显微镜下孵育和计数。

公开(公告)号：US4812414A

公开(公告)日：1989-03-14

申请号：US98250

申请日：1987-09-18

申请人： Harold C. Warren, III ,  Brian A. Snyder

发明人： Harold C. Warren, III ,  Brian A. Snyder

IPC分类号： G01N33/543 ,  G01N33/546 ,  G01N33/547 ,  G01N33/569

CPC分类号： G01N33/54313 ,  Y10S436/825

摘要： A reagent useful in the determination of an immunoreactive species comprises water-insoluble particles having tracer molecules associated therewith. Bound to the outer surface of the particles are: (i) receptor molecules which are reactive with the species, and (ii) molecules of a protein having a pI less than about 6, and which protein is not reactive with either the species or the receptor molecules. The weight ratio of the receptor molecules to the low pI protein molecules is from about 100:1 to about 1:10. This reagent is useful in agglutination and other immunological reactions. A method of preparing the reagent includes providing a suspension of the particles and contacting them with the receptor molecules and low pI protein so as to attach both to the particles. The protein is present in the suspension in an amount such that substantially all of it is attached to the particles. The reagent thus prepared can be used, for example, in agglutination assays for the determination of a multivalent immunoreactive species, such as Streptococcus A antigen.

摘要翻译： 可用于测定免疫反应性物质的试剂包括具有与其相关的示踪分子的水不溶性颗粒。 与颗粒的外表面结合是：（i）与物质反应的受体分子，和（ii）pI小于约6的蛋白质的分子，并且哪种蛋白质不与物种或 受体分子。 受体分子与低pI蛋白质分子的重量比为约100:1至约1:10。 该试剂可用于凝集和其他免疫反应。 制备试剂的方法包括提供颗粒的悬浮液并使其与受体分子和低pI蛋白质接触，以便将它们附着到颗粒上。 蛋白质以一定量使其存在于悬浮液中，使得其基本上全部附着于颗粒。 如此制备的试剂可以用于例如用于测定多价免疫反应物质例如链球菌A抗原的凝集测定。

公开(公告)号：US4810629A

公开(公告)日：1989-03-07

申请号：US780628

申请日：1985-09-26

申请人： John H. Brewer ,  Terry L. Foster

发明人： John H. Brewer ,  Terry L. Foster

IPC分类号： G01N33/551 ,  G01N33/546 ,  G01N33/547

CPC分类号： G01N33/551

摘要： The disclosure is of an improved method for the in-vitro detection and identification of viral associated immunoreactants in biological fluids including cell cultures. The improvement comprises admixing the fluid with solid, porous carrier particles bearing surface adsorbed, known immunological homolog/surfactant complexes. An agglutination reaction indicates the presence of immunoreactant corresponding to the surface adsorbed homolog.

摘要翻译： 本公开是用于体外检测和鉴定包括细胞培养物在内的生物流体中的病毒相关免疫反应物的改进方法。 该改进包括将流体与承载表面吸附的固体，多孔载体颗粒混合，已知的免疫同源物/表面活性剂复合物。 凝集反应表明存在对应于表面吸附同系物的免疫反应物。

公开(公告)号：US4549011A

公开(公告)日：1985-10-22

申请号：US533770

申请日：1983-09-19

申请人： Max Herzberg ,  Falk Fish

发明人： Max Herzberg ,  Falk Fish

IPC分类号： G01N33/547 ,  A61F13/00 ,  A61K39/00 ,  B01D15/08 ,  B01D71/10 ,  B01J20/28 ,  C08L1/02 ,  C12Q1/00 ,  G01N33/543 ,  G01N33/558

CPC分类号： B01D71/10 ,  B01D15/3804 ,  B01D61/00 ,  B01D67/0093 ,  B01J20/28033 ,  G01N33/54353 ,  G01N33/558 ,  B01D2311/2626

摘要： A sheet for separating and retaining biological molecules. The sheet is activated with a compound for covalently binding a ligand to said sheet, and then coated with ligands having an affinity for the substance of interest. A method of using the sheet for isolating and separating substances of interest and methods for forming the sheet.

摘要翻译： 用于分离和保留生物分子的片材。 该片用化合物活化，用于将配体共价结合到所述片材上，然后用对目标物质具有亲和性的配体包被。 用于分离和分离感兴趣物质的片材的方法和用于形成片材的方法。

公开(公告)号：US4539294A

公开(公告)日：1985-09-03

申请号：US431891

申请日：1982-09-30

申请人： Lincoln D. Metcalfe ,  Dieter Frank

发明人： Lincoln D. Metcalfe ,  Dieter Frank

IPC分类号： C08F8/00 ,  A61K38/24 ,  A61K38/44 ,  A61K38/46 ,  C07K14/575 ,  C07K17/08 ,  C08G64/42 ,  C12N11/00 ,  C12N11/02 ,  C12N11/08 ,  C12N11/14 ,  C12N11/18 ,  G01N33/547 ,  C07G7/00 ,  C12N11/06

CPC分类号： C12N11/08 ,  C12N11/02 ,  C12N11/14 ,  C12N11/18 ,  Y10S530/815

摘要： A protein is immobilized by a simple and effective method on a porous polymeric support by a first soaking in a dilute long-chain cationic solution and a second soaking in a dilute aqueous protein solution. The long chain cationic is preferably a nitrogen compound such as a diamine having at least one alkyl or alkenyl group containing at least eight carbon atoms. A preferred diamine is N-coco-1,3-diamino-propane. The protein may be an enzyme or hormone. A preferred enzyme is catalase or a two enzyme system of catalase and glucose oxidase.

摘要翻译： 通过简单有效的方法将蛋白质固定在多孔聚合物载体上，首先在稀释的长链阳离子溶液中浸泡，并在稀释的蛋白质水溶液中浸泡。 长链阳离子优选为氮化合物，例如具有至少一个含有至少8个碳原子的烷基或烯基的二胺。 优选的二胺是N-椰油-1,3-二氨基丙烷。 蛋白质可以是酶或激素。 优选的酶是过氧化氢酶或过氧化氢酶和葡萄糖氧化酶的两个酶系统。

公开(公告)号：US4181636A

公开(公告)日：1980-01-01

申请号：US941344

申请日：1978-09-11

申请人： Ernst A. Fischer

发明人： Ernst A. Fischer

IPC分类号： G01N33/543 ,  G01N33/547 ,  C08L89/00

CPC分类号： G01N33/54353 ,  Y10S436/823

摘要： A process is disclosed for producing an immunological dignostic reagent by mixing an immunologically inert carboxylated latex polymer substantially free from any contaminating amino compound with a water-soluble activating agent and adding an immunologically active material thereto.

摘要翻译： 公开了通过将基本上不含任何污染性氨基化合物的免疫惰性羧化胶乳聚合物与水溶性活化剂混合并向其中加入免疫活性材料来生产免疫性恶臭试剂的方法。