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91.发明授权
公开(公告)号：US4657865A
公开(公告)日：1987-04-14
申请号：US719244
申请日：1985-04-02
申请人： Yoshiyuki Takasaki
发明人： Yoshiyuki Takasaki
CPC分类号： C12P19/16 , C12N9/2417 , C12N9/2457 , C12Y302/01041 , Y10S435/839
摘要： A method for the production of a pullulanase-like enzyme possessing an .alpha.-amylase activity from a strain of genus Bacillus subtilis, the produced enzyme being capable of acting on starch to enhance the yield of glucose.
摘要翻译： 一种生产具有来自枯草芽孢杆菌属菌株的α-淀粉酶活性的支链淀粉酶样酶的方法，所产生的酶能够作用于淀粉以提高葡萄糖的产量。
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92.发明授权Plasmids containing a gene coding for a thermostable pullulanase and pullulanase-producing strains of Escherichia coli and Bacillus subtilis containing the plasmids 失效含有编码热稳定支链淀粉酶和产生支链淀粉酶的大肠杆菌和含有质粒的枯草芽孢杆菌的基因的质粒
公开(公告)号：US4612287A
公开(公告)日：1986-09-16
申请号：US737312
申请日：1985-05-23
CPC分类号： C12N15/75 , C12N9/2457 , C12Y302/01041 , Y10S435/839 , Y10S435/849
摘要： The gene coding for a thermostable pullulanase enzyme is incorporated into chimeric plasmids which are inserted into and reproduced by E. coli or B. subtilis host microorganisms. When microorganisms containing the chimeric plasmids are grown in fermentation media, they produce the pullulanase enzyme.
摘要翻译： 将编码热稳定的支链淀粉酶的基因并入插入大肠杆菌或枯草芽孢杆菌宿主微生物并复制的嵌合质粒中。 当含有嵌合质粒的微生物在发酵培养基中生长时，它们产生支链淀粉酶。
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93.发明授权
公开(公告)号：US4355110A
公开(公告)日：1982-10-19
申请号：US263154
申请日：1981-05-13
CPC分类号： C12Y302/01041 , C12C12/02 , C12C5/004 , C12C7/047 , C12N9/2422 , C12N9/2457 , Y10S435/814 , Y10S435/816
摘要： A debranching enzyme (pullulanese) useful in the preparation of a low calorie beer may be obtained from rice by extraction of the rice with an aqueous buffer system having a pH of about 6. A preferred buffer system is 0.1 M potassium phosphate-0.2 M-sodium chloride. Extraction is preferably carried out at a temperature of about 50.degree. C. for about 3 hours. When malted rice is used as the enzyme source a particularly useful mixture of the debranching enzyme and alpha 1,4 carbohydrases is obtained.
摘要翻译： 用于制备低热量啤酒的脱支酶（支链淀粉）可以通过用pH为约6的水性缓冲体系提取米来从水稻获得。优选的缓冲体系是0.1M磷酸钾-0.2M- 氯化钠。 提取优选在约50℃的温度下进行约3小时。 当麦芽米作为酶源时，可获得脱支酶和α1b糖酶的特别有用的混合物。
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94.发明授权Method for manufacture of maltose from starch by enzymes co-produced by a single microorganism 失效通过单一微生物共同生产的酶从淀粉制备麦芽糖的方法
公开(公告)号：US3992261A
公开(公告)日：1976-11-16
申请号：US538918
申请日：1975-01-06
CPC分类号： C13K7/00 , C12N9/2425 , C12N9/2451 , C12Y302/01002 , C12Y302/01033 , Y10S435/832 , Y10S435/834
摘要： A microorganism which simultaneously produces .beta.-amylase and dextrin .alpha.-1,6-glucosidase is cultured. By use of the enzymes thus produced, starch is directly hydrolyzed into maltose in a high yield.
摘要翻译： 培养同时产生β-淀粉酶和糊精α-1,6-葡糖苷酶的微生物。 通过使用由此产生的酶，淀粉以高产率直接水解成麦芽糖。
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公开(公告)号：US3933588A
公开(公告)日：1976-01-20
申请号：US499818
申请日：1974-08-23
CPC分类号： C12N11/10 , C12N9/2414 , C12N9/2451 , C12N9/2457 , C12N9/96 , C12Y302/01041
摘要： Upon storage, the activity of immobilized enzymes slowly decreases in the presence of moisture. This decrease in activity can be reduced by a significant extent by treating the immobilized enzymes in the presence of moisture with a long chain quaternary ammonium compound.
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公开(公告)号：US3801461A
公开(公告)日：1974-04-02
申请号：US3801461D
申请日：1971-08-31
申请人： HAYASHIBARA CO
发明人： MIYAKE T , SHIOSAKA M
CPC分类号： C12N9/10 , C12N9/00 , C12N9/2457 , C12Y302/01041 , Y10S435/814
摘要： The present invention relates to a process for the extraction of enzymes from microorganisms which is characterized in that an urea solution is used in the extraction of intracellular enzymes produced in cells of microorganisms.
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公开(公告)号：US3716455A
公开(公告)日：1973-02-13
申请号：US3716455D
申请日：1968-08-01
申请人： HAYASHIBARA CO
CPC分类号： C12Y302/01041 , C12N9/2457 , C12N9/246 , C12Y302/01068 , Y10S435/815 , Y10S435/848
摘要： PRODUCTION OF ISOAMYLASE BY FERMENTATION OF A MICROORGANISM BELONGING TO ESCHERICHIA INTERMEDIA IN A CULTURING MEDIUM AT 20*-40*C. AND AT PH 6-9. THE ISOAMYLASE IS AN ENZYME WHICH DECOMPOSES THE A-1,-6-LINKAGE OF STARCH.
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公开(公告)号：US3654089A
公开(公告)日：1972-04-04
申请号：US3654089D
申请日：1969-10-22
申请人： STALEY MFG CO A E
发明人： COKER LOWELL E , TURNER ALMERIN W
CPC分类号： C12Y302/01041 , C12N9/2457 , Y10S435/822
摘要： UTILIZATION OF PHYTATE FILTRATE AND/OR STEP LIQUOR AS THE PRINCIPAL NITROGENOUS MATERIAL FOR THE PRODUCTION OF AMYLO-1,6-GLUCOSIDASE, COMPRISING (1) BRINGING TOGETHER IN AN AQUEOUS MEDIUM INDUCED CELLS CAPABLE OF PRODUCING AMYLO-1,6-GLUCOSIDASE AND AT LEAST ONE NITROGENOUS MATERIAL SELECTED FROM THE GROUP CONSISTING OF STEEP LIQUOUR AND PHYTATE FILTRATE AND (2) INCUBATING AT PH 6.0 TO 8.1 TO PRODUCE AMYLO-1,6-GLUCOSIDASE.
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公开(公告)号：US3654087A
公开(公告)日：1972-04-04
申请号：US3654087D
申请日：1969-10-22
申请人： STALEY MFG CO A E
发明人： COKER LOWELL E , TURNER ALMERIN W
CPC分类号： C12Y302/01041 , C12N9/2457 , Y10S435/822
摘要： METHOD OF PRODUCING AMYLO-1,6-GLUCOSIDASE, WHEREIN AN INOCULUM OF A CULTURE CAPABLE OF PRODUCING AMYLO-1,6GLUCOSIDASE, IS PROPAGATED WITHOUT FORMING ENZYME IN AN AQUEOUS MEDIUM SUBSTANTIALLY FREE OF MALTOSE, MALTOTRIOSE AND PULLULAN COMPRISING DEXTROSE AS THE PRINCIPAL CARBOHYDRATE SOURCE AND INDUCING THE CELLS TO FORM AMYLO1,6-GLUCOSIDASE IN AN AQUEOUS MEDIUM CONTAINING MALTOSE, MALTOTRIOSE OR PULLUIAN.
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公开(公告)号：US3632475A
公开(公告)日：1972-01-04
申请号：US3632475D
申请日：1969-04-18
申请人： HAYASHIBARA CO
CPC分类号： C12P19/16 , C08B30/20 , C12N9/2451 , C12Y302/01033 , Y10S435/822 , Y10S435/831 , Y10S435/832 , Y10S435/847 , Y10S435/848 , Y10S435/853 , Y10S435/859 , Y10S435/866 , Y10S435/874 , Y10S435/882 , Y10S435/885
摘要： The present invention makes it possible to separate short-chainlength amyloses and long-chain-length amyloses from starches by decomposing and cutting off the Alpha -1,6-glucoside bonds of amylopectin contained in starches by means of an Alpha -1,6glucosidase to form short-chain-length amyloses differing in molecular weight from the naturally occuring amyloses and making use of the difference in molecular weight in those two shorts of amyloses.
摘要翻译： 本发明通过分解和切断淀粉中所含的支链淀粉的α-1,6-葡萄糖苷键，可以通过α-1分离出短链长度的直链淀粉和长链长度的淀粉， 从而形成与天然存在的直链淀粉不同分子量的短链长度的直链淀粉，并利用这两条直链淀粉的分子量的差异。
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