公开(公告)号：US11505789B2

公开(公告)日：2022-11-22

申请号：US16971169

申请日：2019-02-19

申请人： National University Corporation Hokkaido University ,  Nihon Shokuhin Kako Co., Ltd.

发明人： Haruhide Mori ,  Wataru Saburi ,  Kenta Kanai ,  Kenta Aizawa ,  Takahisa Iizuka ,  Noriaki Takechi ,  Mioka Tani

IPC分类号： C12N9/44 ,  C12P19/18

摘要： The present invention relates to an enzyme having α-1,6-glucosyl transfer activity, which can use a partially degraded starch product as a substrate and is heat resistant and suitable for industrial applications; an enzyme preparation for use in manufacturing α-1,6-glucan, comprising the enzyme as an active ingredient; and a method for manufacturing α-1,6-glucan using the enzyme or enzyme preparation. The present invention provides an enzyme having α-1,6-glucosyl transfer activity, which is any one of proteins (a), (b), and (c): (a) a protein consisting of an amino acid sequence of SEQ ID NO: 3; (b) a protein consisting of an amino acid sequence having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 3; and (c) a protein consisting of an amino acid sequence in which one or several amino acid(s) have been substituted, inserted, deleted and/or added in the amino acid sequence of SEQ ID NO: 3.

公开(公告)号：US20220275112A1

公开(公告)日：2022-09-01

申请号：US17680581

申请日：2022-02-25

申请人： NUTRITION & BIOSCIENCES USA 4, INC.

发明人： Qiong Cheng ,  Robert DiCosimo ,  Arthur Ouwehand ,  Zheng You ,  Mark S. Payne ,  Jian Ping Lai ,  Kristin Ruebling-Jass ,  Steven Cary Rothman

IPC分类号： C08B37/00 ,  A23L29/30 ,  A23L33/21 ,  A61K31/716 ,  C12P19/04 ,  C12P19/08 ,  C12P19/18 ,  C08L5/00 ,  A23L7/135 ,  A23L7/126 ,  A23L21/10 ,  A21D13/45 ,  A21D13/80 ,  A23C9/13 ,  A23G3/36 ,  A23G9/32 ,  A23L2/52 ,  C12N9/10 ,  C12N9/44

摘要： An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

公开(公告)号：US20220098630A1

公开(公告)日：2022-03-31

申请号：US17415640

申请日：2019-12-18

申请人： GreenLight Biosciences, Inc.

发明人： Daniel MacEachran ,  Drew S. Cunningham ,  William Jeremy Blake ,  Matthew Eduardo Moura

IPC分类号： C12P19/02 ,  C12N9/10 ,  C12N9/90 ,  C12N9/16 ,  C12N9/44 ,  C12N1/20 ,  C12N1/16 ,  C12N1/06 ,  C12P19/24

摘要： Provided herein, in some embodiments, are cell-free systems, methods, kits, and compositions (e.g., cells and cell lysates) for converting a polysaccharide to allulose via the use of enzymes, such as thermostable enzymes.

公开(公告)号：US11261264B2

公开(公告)日：2022-03-01

申请号：US16451501

申请日：2019-06-25

申请人： NUTRITION & BIOSCIENCES USA 4, INC.

发明人： Qiong Cheng ,  Robert DiCosimo ,  Arthur Ouwehand ,  Zheng You ,  Mark S. Payne ,  Jian Ping Lai ,  Kristin Ruebling-Jass ,  Steven Cary Rothman

IPC分类号： C08B37/00 ,  A21D13/45 ,  A21D13/80 ,  A23C9/13 ,  A23G3/36 ,  A23G9/32 ,  A23L2/52 ,  A23L2/10 ,  C08L5/00 ,  C12N9/10 ,  C12N9/44 ,  C12P19/04 ,  C12P19/08 ,  A23L29/30 ,  A23L33/21 ,  A61K31/716 ,  C12P19/18 ,  A23L7/135 ,  A23L7/126 ,  A23L21/10

摘要： An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

公开(公告)号：US11155847B2

公开(公告)日：2021-10-26

申请号：US15505536

申请日：2015-08-22

申请人： ISOThrive LLC ,  Board of Supervisors of Louisiana State University and Agricultural and Mechanical College

发明人： Lee Madsen, II ,  Jack Oswald ,  Donal F Day ,  Young Hwan Moon

IPC分类号： C12P19/04 ,  C12P19/08 ,  C08B37/00 ,  C12N9/44 ,  C12P19/18 ,  C12N1/20 ,  A61K31/715 ,  C12P19/20 ,  C12R1/01

摘要： The present invention provides a method for the production of oligosaccharides by the fermentation of dextran-sucrase-producing microorganisms with sucrose and maltose. The disclosed process allows for the control of the final composition of the isomaltooligosaccharides by adjustments to pH and the initial ratio of sucrose to maltose.

公开(公告)号：US10975366B2

公开(公告)日：2021-04-13

申请号：US16476878

申请日：2017-10-24

申请人： GUANGDONG VTR BIO-TECH CO., LTD

发明人： Yangyuan Li ,  Jianrong Wang ,  Ling Yang ,  Man Qu ,  Lizhi Chen ,  Jiale Huang

IPC分类号： C12N9/54 ,  C12N9/44

摘要： The present invention relates to the field of genetic engineering, particularly to a method for efficiently expressing pullulanase in Bacillus subtilis and recombinant Bacillus subtilis. said method includes steps of constructing modified Bacillus subtilis strain with deletion of alkaline protease gene and neutral protease gene, constructing expression vector including an optimized combination of promoter and signal peptide and pullulanase gene, and transforming said modified Bacillus subtilis strain with by said expression vector. A series of combinations of promoter and signal peptide are optimized to obtain the combination for efficiently expressing pullulanase, provide an industrial application basis.

公开(公告)号：US10808271B2

公开(公告)日：2020-10-20

申请号：US16305272

申请日：2017-05-31

申请人： NESTEC S.A.

发明人： Lubbert Dijkhuizen ,  Joana Gangoiti Munecas ,  Sander Sebastiaan Van Leeuwen ,  Christina Vafeiadi ,  Stephane Duboux

IPC分类号： C12P19/18 ,  C12N9/26 ,  C12N9/44 ,  C12P19/00 ,  A23L33/21 ,  A23K20/189 ,  C12P19/04

摘要： The present invention relates to the field of poly- and oligosaccharides and their dietary effects. In particular it relates to a method of producing an α-glucan containing (α1→3) linked D-glucose units. The invention also provides a branched α-glucan comprising alternating (α1→4) and (α1→3) glucosidic linkages and having (α1→3,4) branching points, a food composition, and the use of an α-glucanotransferase enzyme for reducing the digestible carbohydrates of a starch containing food material. Further aspects of the invention are a bacteria, an enzyme and an expression vector.

公开(公告)号：US10793844B2

公开(公告)日：2020-10-06

申请号：US16154205

申请日：2018-10-08

申请人： BASF Enzymes LLC

发明人： Kevin A. Gray ,  Nahla M. Aboushadi ,  James B. Garrett

IPC分类号： C12N9/26 ,  A23L29/00 ,  A23L29/30 ,  A23K20/189 ,  A23K10/14 ,  C12N9/28 ,  A21D8/04 ,  C11D3/386 ,  C12C5/00 ,  C12N9/34 ,  C12N9/44

摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.

公开(公告)号：US20200165586A1

公开(公告)日：2020-05-28

申请号：US16787203

申请日：2020-02-11

申请人： NOVOZYMES A/S

发明人： Joseph Jump ,  Nathaniel Edward Kreel ,  Bernardo Vidal, JR. ,  Chee-Leong Soong ,  Madison Roberts ,  Melissa Carrie Hooss ,  Xinyu Shen

IPC分类号： C12N9/16 ,  C12N9/28 ,  C12P7/06 ,  C12N9/20 ,  C12N9/50 ,  C12N9/44 ,  C12N9/42 ,  C12N9/34 ,  C12N9/26 ,  C12P19/14

摘要： A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c). Use of a protease and a phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.

公开(公告)号：US10584324B2

公开(公告)日：2020-03-10

申请号：US15520567

申请日：2015-10-23

申请人： NOVOZYMES A/S

发明人： Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC分类号： C12N9/34 ,  C12P19/14 ,  C12N15/56 ,  C12N1/15 ,  C12P19/02 ,  C12P7/06 ,  C12N9/30 ,  C12N9/44 ,  C12P7/14

摘要： The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.