公开(公告)号：US11851679B2

公开(公告)日：2023-12-26

申请号：US16760006

申请日：2018-11-01

申请人： Juno Therapeutics, Inc.

发明人： Rupesh Amin ,  Aye Chen

IPC分类号： C12N5/0783 ,  C07K14/725 ,  C12N15/113 ,  C12N15/65 ,  C12N15/86

CPC分类号： C12N5/0636 ,  C07K14/7051 ,  C12N15/113 ,  C12N15/65 ,  C12N15/86 ,  C07K2319/03 ,  C12N2740/16043

摘要： Provided herein are reporter T-cells containing a reporter operably linked to the Nur77 locus. Also provided are methods for screening for an activity of a recombinant receptor, including those containing an extracellular antigen-binding domain and an intracellular signaling domain, such as a chimeric antigen receptor (CAR), including assessing activity of a cell expressing the recombinant receptor based on a detectable expression of a reporter molecule responsive to a signal through the intracellular signaling region of the recombinant receptor. In some embodiments, the activity assessed is an antigen-dependent or an antigen-independent activity. In some embodiments, the methods can be used to screen a plurality of reporter cells each containing a nucleic acid molecule encoding a candidate recombinant receptor, e.g. CAR, and assessing such cells for one or more property or activity. The methods can be high-throughput. Also provided are reporter cells, cell compositions, nucleic acids and kits for use in the methods.

公开(公告)号：US11851678B2

公开(公告)日：2023-12-26

申请号：US16637709

申请日：2018-08-09

申请人： Juno Therapeutics, Inc.

发明人： Mirna Mujacic ,  Ayu Rahardjo

IPC分类号： A61K35/17 ,  A61K38/19 ,  C12N5/0783 ,  A61K38/20 ,  C07K14/705 ,  C12N7/00 ,  C12N15/86 ,  G01N33/569

CPC分类号： C12N5/0636 ,  A61K35/17 ,  C12N7/00 ,  C12N15/86 ,  G01N33/56972 ,  C12N2502/1114 ,  C12N2740/13043 ,  C12N2740/15043 ,  G01N2333/70514 ,  G01N2333/70517

摘要： Provided herein are methods and compositions for generating engineered cells, such as cells expressing a recombinant receptor, including methods involving stimulation and/or engineering of an input composition having a defined ratio of naive-like CD4+ T cells to naive-like CD8+ T cells. In particular, the methods can be used to engineer T cells with genetically engineered receptors, such as genetically engineered antigen receptors such as engineered (recombinant) TCRs and chimeric antigen receptors (CARs), or other recombinant chimeric receptors. Features of the methods include producing a more consistent and/or predictable T cell product and/or a product with lower toxicity compared with other methods.

公开(公告)号：US11844793B2

公开(公告)日：2023-12-19

申请号：US17449412

申请日：2021-09-29

申请人： AeroRx Therapeutics LLC

发明人： John Chan ,  Keith Try Ung ,  Mei-Chang Kuo ,  John Nigel Pritchard

IPC分类号： A61K31/4704 ,  A61K31/5386 ,  A61K31/40 ,  A61K9/00 ,  A61K47/69 ,  A61K47/26 ,  A61K31/58 ,  A61K9/08 ,  A61K9/12 ,  A61K47/10 ,  A61P11/00

CPC分类号： A61K31/4704 ,  A61K9/0078 ,  A61K9/08 ,  A61K9/12 ,  A61K31/40 ,  A61K31/5386 ,  A61K31/58 ,  A61K47/10 ,  A61K47/26 ,  A61K47/6951 ,  A61P11/00

摘要： Aqueous formulations of indacaterol are disclosed. The formulations may find use in the treatment of respiratory disorders, inflammatory disorders, or obstructive airway diseases. Methods of using the formulations and kits comprising the formulations are also encompassed by the disclosure.

公开(公告)号：US11844318B2

公开(公告)日：2023-12-19

申请号：US17263101

申请日：2019-07-25

申请人： Invaio Sciences International GmbH

发明人： Lukas Rudolf Schupbach ,  Urs Widmer ,  Michael Christian Oehl ,  Antony Mathai Chettoor

IPC分类号： A01G7/06

CPC分类号： A01G7/06

摘要： A plant injection system includes an injection tool configured to penetrate a plant and distribute a liquid formulation to the plant. The injection tool includes abase having an inlet port and a penetrating distribution body extending along a longitudinal body axis. The penetrating distribution body includes a penetrating element and one or more distribution ports in communication with the inlet port. The one or more distribution ports are spaced from the penetrating element. The injection tool includes penetrating and distribution configurations. In the penetrating configuration the penetrating element is configured to penetrate the plant along the longitudinal body axis. In the distribution configuration the one or more distribution ports are configured to distribute the liquid formulation to the plant transversely relative to one or more penetrating directions, at least one of the penetrating directions corresponding to the longitudinal body axis.

公开(公告)号：US11836775B2

公开(公告)日：2023-12-05

申请号：US18151492

申请日：2023-01-09

申请人： MDSAVE SHARED SERVICES INC.

发明人： Paul J. Ketchel, III ,  Ryan Aipperspach ,  Karen Martirosyan ,  Ani Osborne ,  Mark Kaufman ,  Michael Bischoff ,  Daniel Schmidt

IPC分类号： G06Q40/00 ,  G06Q30/0601 ,  G06Q50/22 ,  G06Q30/0201 ,  G06Q30/0207 ,  G06Q20/06 ,  G06Q20/10 ,  G06Q20/38 ,  G16H40/20 ,  G16H10/60 ,  G06Q40/08 ,  G16H20/00

CPC分类号： G06Q30/0621 ,  G06Q20/065 ,  G06Q20/10 ,  G06Q20/381 ,  G06Q30/0206 ,  G06Q30/0239 ,  G06Q30/0613 ,  G06Q30/0629 ,  G06Q30/0633 ,  G06Q40/08 ,  G06Q50/22 ,  G16H10/60 ,  G16H40/20 ,  G16H20/00

摘要： Apparatus and associated methods relate to presenting for selection, services comprising at least one bundled set of healthcare services to be performed separately by respective providers, determining a bundle price for the at least one bundled set of healthcare services and in response to receiving payment in an amount of the bundle price, generating a persistent purchase data record with a unique confirmation number that is selectively redeemable by the user to receive each of the healthcare services in the bundled set. The bundle price may be discounted and/or based on the location or time at which at least one service will be performed. The bundle price may also be based on the user's remaining health insurance deductible. A single payment may be disbursed to multiple providers of the bundled set of healthcare services. The received and/or disbursed payment may be in virtual funds.

公开(公告)号：US11835434B2

公开(公告)日：2023-12-05

申请号：US17125794

申请日：2020-12-17

申请人： Genzyme Corporation

发明人： Martha Stapels ,  Michelle Busch

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  G01N2458/15 ,  G01N2560/00

摘要： The present invention provides methods for improved label-free absolute quantification of relatively low abundant polypeptides by liquid chromatography/mass spectrometry analysis of peptide products obtained from simple or complex polypeptide mixtures. The methods for absolute quantification include MS signals from a set of qualified ions of peptide products of a relatively high abundant polypeptide to improve quantification of a relatively low abundant polypeptide.

公开(公告)号：US11834675B2

公开(公告)日：2023-12-05

申请号：US16067536

申请日：2016-12-29

申请人： CELGENE CORPORATION

发明人： Bitao Liang ,  Xiaohua Lu ,  Wei Liu ,  Kathy E. Karasciewicz-Mendez ,  Christopher Wiwi ,  Kruti Shah

IPC分类号： C12N5/0783 ,  A61K39/00 ,  A61K35/17 ,  C07K16/28

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K39/0011 ,  C07K16/2818 ,  C07K16/2827 ,  C12N2501/2304 ,  C12N2501/2307 ,  C12N2501/2315

摘要： Provided herein are methods of generating T cells, e.g., cytotoxic T lymphocytes starting from peripheral blood mononuclear cells without a separate step of generating and isolating antigen-presenting cells, and with a single round of antigen stimulation. Also provided herein are methods of using said cytotoxic T lymphocytes, for example, to treat cancer and/or viral infection.

公开(公告)号：US11834648B2

公开(公告)日：2023-12-05

申请号：US16761935

申请日：2017-11-06

申请人： SHENZHEN CELL INSPIRE BIOTECHNOLOGY CO., LTD.

发明人： Yubo Yan ,  Jian Fu ,  Min Zhou ,  Yuqing Liu ,  Lixiang Jiang ,  Bo Yang ,  Jiayin Yang

IPC分类号： C12N5/074 ,  C12N5/0793 ,  C12N15/90 ,  G01N33/50 ,  G01N33/68

CPC分类号： C12N5/0696 ,  C12N5/0619 ,  C12N15/90 ,  G01N33/5058 ,  G01N33/6896 ,  C12N2503/02 ,  C12N2506/025 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2740/10043 ,  G01N2333/4709

摘要： A method of generating a cellular model of Alzheimer's disease (AD) comprises integrating AD related gene to hiPSC to induce increased beta secretase and/or Abeta 42 peptides, and the cellular model of Alzheimer's disease (AD) is prepared by the method.

公开(公告)号：US11834490B2

公开(公告)日：2023-12-05

申请号：US16321000

申请日：2017-07-27

申请人： ALPINE IMMUNE SCIENCES, INC.

发明人： Ryan Swanson ,  Michael Kornacker ,  Daniel William Demonte ,  Mark F. Maurer ,  Dan Ardourel ,  Joseph L. Kuijper

IPC分类号： A61K39/00 ,  C07K14/705 ,  A61K35/12 ,  C07K14/725 ,  C12N15/85 ,  A61K38/00

CPC分类号： C07K14/70596 ,  A61K35/12 ,  C07K14/7051 ,  C12N15/85 ,  A61K38/00 ,  C07K2319/03 ,  C07K2319/30 ,  C12N2510/00

摘要： Provided herein are immunomodulatory proteins comprising variant CD112 and nucleic acids encoding such proteins. The immunomodulatory proteins provide therapeutic utility for a variety of immunological and oncological conditions. Compositions and methods for making and using such proteins are provided.

公开(公告)号：US11832598B2

公开(公告)日：2023-12-05

申请号：US16878362

申请日：2020-05-19

申请人： Akeagen, Inc.

发明人： Libin Cui ,  Xiaolin Sun

IPC分类号： A01K67/027 ,  C07K16/28 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C07K16/00

CPC分类号： A01K67/0276 ,  C07K16/00 ,  C07K16/2818 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2227/107 ,  A01K2267/01 ,  C07K2317/10 ,  C07K2317/51 ,  C07K2317/522 ,  C07K2317/92 ,  C12N2310/20 ,  C12N2800/80

摘要： The present application provides genetically modified non-human animals and methods for producing heavy chain-only antibodies (HcAbs), wherein the genetically modified non-human animal comprises a germline genome comprising an engineered immunoglobulin heavy chain (IgH) allele at an endogenous IgH locus, wherein the engineered IgH allele lacks functional gene segments encoding CH1 domains of all endogenous IgG subclasses. In some embodiments, a genetically modified mouse is provided, comprising an engineered IgH allele that lacks a functional endogenous gene segment encoding Cγ3, Cγ1, Cγ2b and CH1 exon of Cγ2c. Further provided are HcAbs or derivatives thereof produced by the genetically modified non-human animals.