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145 条记录 (耗时 0.033 秒)

    METHODS AND DEVICES FOR CONTROLLING TEMPERATURE WITHOUT TEMPERATURE SENSOR
    104.
    发明申请
    METHODS AND DEVICES FOR CONTROLLING TEMPERATURE WITHOUT TEMPERATURE SENSOR 审中-公开
    用于控制温度无温度传感器的方法和装置

    公开(公告)号：US20090039073A1

    公开(公告)日：2009-02-12

    申请号：US12088422

    申请日：2006-12-22

    申请人： Min Guo ,  Keyue Shen ,  Cheng Zhou ,  Xiaosheng Guan ,  Jing Cheng

    发明人： Min Guo ,  Keyue Shen ,  Cheng Zhou ,  Xiaosheng Guan ,  Jing Cheng

    IPC分类号： H05B1/02 ,  G05D23/00 ,  H05B3/40

    CPC分类号： G01K7/18 ,  G01K7/183 ,  G05D23/2401

    摘要： Methods and devices for controlling temperature by precise heating without the need of using a temperature sensor are provided. The device comprises a resistive heating element (6), a controller (3), a heating circuit and a temperature sensing circuit. The temperature of the resistive heating element can be determined based on the resistance of the resistive heating element (6), which changes monotonically with its temperature. The resistive heating element (6) thus serves both as a heating element and as a temperature sensor, thereby obviating the need for a separate temperature sensor.

    摘要翻译： 提供了通过精确加热来控制温度而不需要使用温度传感器的方法和装置。 该装置包括电阻加热元件（6），控制器（3），加热电路和温度检测电路。 电阻加热元件的温度可以基于其温度单调变化的电阻加热元件（6）的电阻来确定。 因此，电阻加热元件（6）既用作加热元件又用作温度传感器，从而避免需要单独的温度传感器。

    Testing Method of Nucleic Acid Binding Protein Based on Biochip
    105.
    发明申请
    Testing Method of Nucleic Acid Binding Protein Based on Biochip 有权
    基于生物芯片的核酸结合蛋白检测方法

    公开(公告)号：US20090018025A1

    公开(公告)日：2009-01-15

    申请号：US11791157

    申请日：2004-11-23

    申请人： Wei Shao ,  Yongchao Zhao ,  Yimin Sun ,  Jiying Qiao ,  Huajiang Wei ,  Weiping Yang ,  Yuxiang Zhou ,  Jing Cheng

    发明人： Wei Shao ,  Yongchao Zhao ,  Yimin Sun ,  Jiying Qiao ,  Huajiang Wei ,  Weiping Yang ,  Yuxiang Zhou ,  Jing Cheng

    IPC分类号： C40B20/02

    CPC分类号： G01N33/54366 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2565/531 ,  C12Q2565/501 ,  C12Q2563/131

    摘要： A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization.

    摘要翻译： 一种基于生物芯片的核酸结合蛋白的检测方法，包括以下步骤：1.将包含核酸捕获探针的多组溶液放入包括待测试的多种核酸结合蛋白的生物样品中，从而形成核酸 捕获的探针 - 核酸结合蛋白复合物; 此类核酸捕获的探针包括可与靶核酸结合蛋白结合的至少一段结合序列; 2.分离这些核酸捕获的探针 - 核酸结合蛋白复合物，然后回收核酸捕获的探针; 3.将根据步骤2的核酸捕获探针与生物芯片底物上的多个单链印迹探针杂交; 这种印迹探针的序列用这种核酸捕获的探针或其一条链补偿; 4.检测杂交结果。

    Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses
    106.
    发明申请
    Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses 审中-公开
    提高细胞电穿孔效率的方法

    公开(公告)号：US20090000948A1

    公开(公告)日：2009-01-01

    申请号：US12097409

    申请日：2007-01-04

    申请人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

    发明人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

    IPC分类号： G01N27/26 ,  G01N27/00

    CPC分类号： C12N13/00 ,  C12M23/16 ,  C12M33/00 ,  C12M35/02

    摘要： The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.

    摘要翻译： 本发明提供使用介电电泳辅助细胞定位提高细胞电穿孔效率的方法及其在微流体生物芯片系统中的用途。 细胞首先进行介导电泳，并定位于电场强度足以使细胞电穿孔的区域。 本发明增强了传统微流体生物芯片上原位细胞电穿孔的效率。

    Nanometer-sized up-converting phosphor fluoride particles and process of preparation
    108.
    发明授权
    Nanometer-sized up-converting phosphor fluoride particles and process of preparation 有权
    纳米级上转换氟化磷颗粒及其制备方法

    公开(公告)号：US07422703B2

    公开(公告)日：2008-09-09

    申请号：US10511363

    申请日：2003-04-15

    申请人： Guangshun Yi ,  Baoquan Sun ,  Depu Chen ,  Yuxiang Zhou ,  Jing Cheng ,  Wenjun Yang ,  Yue Ge ,  Lianghong Guo

    发明人： Guangshun Yi ,  Baoquan Sun ,  Depu Chen ,  Yuxiang Zhou ,  Jing Cheng ,  Wenjun Yang ,  Yue Ge ,  Lianghong Guo

    IPC分类号： C09K11/85

    CPC分类号： C09K11/7772 ,  C09K11/7773 ,  Y10S977/83 ,  Y10S977/834

    摘要： Nanometer-scaled up-converting fluoride phosphor particles and processes of making them are disclosed. In the process, an aqueous solution consisting of soluble salts of rare-earth metal ions at a molar ratio of (yttrium, lanthanum or gadolinium): ytterbium:(erbium, holmium, terbium or thulium)=(70-90):(0-29):(0.001-15) is mixed a rare-earth metal chelator and a soluble fluoride salt to form precipitates, which are then annealed at an elevated temperature to produce nanometer-scaled up-converting fluoride phosphor particles. The particle size is between 35 nm and 200 nm, and can be controlled by the amount of the metal chelator added to the solution. The nanometer-sized particle is applicable to many biological assays.

    摘要翻译： 公开了纳米级上转换氟化物荧光体颗粒及其制备方法。 在该方法中，以（钇，镧或钆）的摩尔比形成的稀土金属离子的可溶性盐：镱（铒，钬，铽或ium）=（70-90）：（0） -29）:( 0.001-15）混合稀土金属螯合剂和可溶性氟化物盐形成析出物，然后在升高的温度下退火，生成纳米级的上转换氟化物荧光体颗粒。 粒径在35nm和200nm之间，并且可以通过添加到溶液中的金属螯合剂的量来控制。 纳米级粒子适用于许多生物测定。