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公开(公告)号：US12018323B2
公开(公告)日：2024-06-25
申请号：US17480067
申请日：2021-09-20
Applicant: Fluidigm Corporation
Inventor： Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
IPC: C12Q1/68 , C12N15/10 , C12N15/65 , C12N15/66 , C12Q1/6855 , C12Q1/686 , C12Q1/6874
CPC classification number: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
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公开(公告)号：US11795494B2
公开(公告)日：2023-10-24
申请号：US17166992
申请日：2021-02-03
Applicant: Fluidigm Corporation
Inventor： Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
IPC: C12Q1/6806 , C12Q1/686 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/6806 , B01L3/502738 , C12Q1/686 , B01L3/50273 , B01L7/52 , B01L2300/087 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629 , C12Q1/6806 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
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公开(公告)号：US10745748B2
公开(公告)日：2020-08-18
申请号：US16134855
申请日：2018-09-18
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: G01N21/00 , C12Q1/686 , G01N21/64 , G01N27/447 , G02B21/16 , G01N21/84 , G01N21/75 , G02B21/36 , B01L3/00 , B01L7/00
Abstract: In some embodiments, a variety of devices and methods for conducting microfluidic analyses are utilized herein, including devices that can be utilized to conduct thermal cycling reactions such as nucleic acid amplification reactions. The devices include elastomeric components; in some instances, much or all of the device is composed of elastomeric material. Amplification products (amplicons) can be detected and distinguished (whether isolated in a reaction chamber or at any subsequent time) using routine methods for detecting nucleic acids. An example of a detection method includes hybridization to arrays of immobilized oligo or polynucleotides.
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公开(公告)号：US10436698B2
公开(公告)日：2019-10-08
申请号：US16230570
申请日：2018-12-21
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
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公开(公告)号：US20190195771A1
公开(公告)日：2019-06-27
申请号：US16230570
申请日：2018-12-21
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
CPC classification number: G01N15/1404 , G01N2015/0065 , G01N2458/15 , H01J49/004 , H01J49/04 , H01J49/0431
Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
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Patent Agency Ranking
公开(公告)号：US10227624B2
公开(公告)日：2019-03-12
申请号：US14800548
申请日：2015-07-15
Applicant: Fluidigm Corporation
Inventor： Peilin Chen , Jing Wang , Andrew May
IPC: C12Q1/68 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/683
Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
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公开(公告)号：US10214773B2
公开(公告)日：2019-02-26
申请号：US15724190
申请日：2017-10-03
Applicant: Fluidigm Corporation
Inventor： Robert C. Jones
IPC: C12Q1/68 , C12Q1/686 , C12Q1/682 , G01N33/532 , G01N33/53
Abstract: Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids (e.g., RNA) and proteins.
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公开(公告)号：US10180386B2
公开(公告)日：2019-01-15
申请号：US15888871
申请日：2018-02-05
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
Abstract: A method for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. Particles or element tags associated with particles can be vaporized, atomized, and ionized, such as with an inductively coupled plasma device or a glow discharge device. The vaporized, atomized, and ionized particles or element tags can be analyzed using mass spectrometry, such as using a time of flight mass spectrometer or a magnetic sector mass spectrometer. The amount of at least one element in individual particles can be measured through mass analysis. The amount of many different tags, for example at least five different tags, can be measured at the same time to facilitate multi-parametric analysis of cells and other particles. The vaporized, atomized, and ionized particles or element tags can be pretreated in an ion pretreatment system to filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough.
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公开(公告)号：US10131934B2
公开(公告)日：2018-11-20
申请号：US15805720
申请日：2017-11-07
Applicant: Fluidigm Corporation
Inventor： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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公开(公告)号：US20180195948A1
公开(公告)日：2018-07-12
申请号：US15888871
申请日：2018-02-05
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
CPC classification number: G01N15/1404 , G01N2015/0065 , G01N2458/15 , H01J49/004 , H01J49/04 , H01J49/0431
Abstract: A method for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. Particles or element tags associated with particles can be vaporized, atomized, and ionized, such as with an inductively coupled plasma device or a glow discharge device. The vaporized, atomized, and ionized particles or element tags can be analyzed using mass spectrometry, such as using a time of flight mass spectrometer or a magnetic sector mass spectrometer. The amount of at least one element in individual particles can be measured through mass analysis. The amount of many different tags, for example at least five different tags, can be measured at the same time to facilitate multi-parametric analysis of cells and other particles. The vaporized, atomized, and ionized particles or element tags can be pretreated in an ion pretreatment system to filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough.
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