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公开(公告)号:US20190169676A1
公开(公告)日:2019-06-06
申请号:US16150096
申请日:2018-10-02
Applicant: Fluidigm Corporation
Inventor: Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
IPC: C12Q1/68 , B01L3/00 , C12Q1/686 , G01N27/453
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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公开(公告)号:US10131934B2
公开(公告)日:2018-11-20
申请号:US15805720
申请日:2017-11-07
Applicant: Fluidigm Corporation
Inventor: Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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公开(公告)号:US20180073051A1
公开(公告)日:2018-03-15
申请号:US15805720
申请日:2017-11-07
Applicant: Fluidigm Corporation
Inventor: Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
CPC classification number: C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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4.
公开(公告)号:US09506914B2
公开(公告)日:2016-11-29
申请号:US14535162
申请日:2014-11-06
Applicant: Fluidigm Corporation
Inventor: Ian D. Manger , Joseph W. Barco , Hany R. Nassef
CPC classification number: B81B3/00 , B01L3/5025 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/088 , B01L2300/0887 , B01L2400/0415 , B01L2400/0481 , B01L2400/0655 , F04B19/006 , F04B43/02 , F16K99/0001 , F16K99/0003 , F16K99/0026 , F16K99/0036 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , G01N33/5304 , G01N33/54366 , Y10T436/2575
Abstract: A microfluidic device includes a plurality of first flow channels and a plurality of second flow channels, each such second flow channel intersecting multiple of the first flow channels to define intersecting volumes and a plurality of looped flow channels that each include segments of the flow channels between the intersecting volumes to define a closed loop. The microfluidic device also includes a plurality of control valves each such control valve having a control channel and a deformable segment disposed to restrict flow through a respective one of the first and second flow channels in response to an actuation force applied to the control channel to deflect the deformable segment. The microfluidic device further includes a pump operatively disposed to regulate flow through one of the looped flow channels to regulate flow by the recirculating pump.
Abstract translation: 微流体装置包括多个第一流动通道和多个第二流动通道,每个这样的第二流动通道与第一流动通道的多个相交,以限定相交的体积,以及多个环形流动通道,每个流动通道包括在 相交的卷定义一个闭环。 微流体装置还包括多个控制阀,每个这样的控制阀具有控制通道和可变形部分,可变形部分设置成响应于施加到控制通道的致动力而限制流过第一和第二流动通道中的相应一个流动通道以偏转 可变形段。 微流体装置还包括一个可操作地设置成用于调节通过环形流动通道之一的流量以调节由再循环泵流动的泵。
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5.
公开(公告)号:US20150168385A1
公开(公告)日:2015-06-18
申请号:US14535162
申请日:2014-11-06
Applicant: Fluidigm Corporation
Inventor: Ian D. Manger , Joseph W. Barco , Hany R. Nassef
IPC: G01N33/53
CPC classification number: B81B3/00 , B01L3/5025 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/088 , B01L2300/0887 , B01L2400/0415 , B01L2400/0481 , B01L2400/0655 , F04B19/006 , F04B43/02 , F16K99/0001 , F16K99/0003 , F16K99/0026 , F16K99/0036 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , G01N33/5304 , G01N33/54366 , Y10T436/2575
Abstract: A microfluidic device includes a plurality of first flow channels and a plurality of second flow channels, each such second flow channel intersecting multiple of the first flow channels to define intersecting volumes and a plurality of looped flow channels that each include segments of the flow channels between the intersecting volumes to define a closed loop. The microfluidic device also includes a plurality of control valves each such control valve having a control channel and a deformable segment disposed to restrict flow through a respective one of the first and second flow channels in response to an actuation force applied to the control channel to deflect the deformable segment. The microfluidic device further includes a pump operatively disposed to regulate flow through one of the looped flow channels to regulate flow by the recirculating pump.
Abstract translation: 微流体装置包括多个第一流动通道和多个第二流动通道,每个这样的第二流动通道与第一流动通道的多个相交,以限定相交的体积,以及多个环形流动通道,每个流动通道包括在 相交的卷定义一个闭环。 微流体装置还包括多个控制阀,每个这样的控制阀具有控制通道和可变形部分,可变形部分设置成响应于施加到控制通道的致动力而限制流过第一和第二流动通道中的相应一个流动通道以偏转 可变形段。 微流体装置还包括一个可操作地设置成用于调节通过环形流动通道之一的流量以调节由再循环泵流动的泵。
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