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公开(公告)号：US09103761B2
公开(公告)日：2015-08-11
申请号：US13867555
申请日：2013-04-22
申请人： Fluidigm Corporation
发明人： Hany Nassef , Geoffrey Facer , Marc Unger
CPC分类号： C03C25/68 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N15/1031 , G01N15/12 , G01N27/00 , G01N27/60 , G01N33/48707 , G01N33/48721 , G01N2015/1087 , Y10T436/2575
摘要： The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.
摘要翻译： 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。
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公开(公告)号：US20130302785A1
公开(公告)日：2013-11-14
申请号：US13867555
申请日：2013-04-22
申请人： Fluidigm Corporation
发明人： Hany Nassef , Geoffrey Facer , Marc Unger
IPC分类号： G01N27/00
CPC分类号： C03C25/68 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N15/1031 , G01N15/12 , G01N27/00 , G01N27/60 , G01N33/48707 , G01N33/48721 , G01N2015/1087 , Y10T436/2575
摘要： The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.
摘要翻译： 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。
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公开(公告)号：US09316331B2
公开(公告)日：2016-04-19
申请号：US14091342
申请日：2013-11-27
申请人： Fluidigm Corporation
发明人： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
IPC分类号： B01L3/00 , G05D11/02 , F16K99/00 , B01F5/02 , B01F13/00 , C12Q1/68 , G01N21/75 , F16K3/00 , G01N21/03
CPC分类号： F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
摘要： Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
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公开(公告)号：US08616227B1
公开(公告)日：2013-12-31
申请号：US13909000
申请日：2013-06-03
申请人： Fluidigm Corporation
发明人： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
CPC分类号： F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
摘要： Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
摘要翻译： 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。
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公开(公告)号：US20130337457A1
公开(公告)日：2013-12-19
申请号：US13909000
申请日：2013-06-03
申请人： Fluidigm Corporation
发明人： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
IPC分类号： C12Q1/68
CPC分类号： F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
摘要： Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
摘要翻译： 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。
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公开(公告)号：US20190169676A1
公开(公告)日：2019-06-06
申请号：US16150096
申请日：2018-10-02
申请人： Fluidigm Corporation
发明人： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
IPC分类号： C12Q1/68 , B01L3/00 , C12Q1/686 , G01N27/453
摘要： A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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公开(公告)号：US20160129441A1
公开(公告)日：2016-05-12
申请号：US14873958
申请日：2015-10-02
申请人： Fluidigm Corporation
IPC分类号： B01L3/00
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
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公开(公告)号：US20160091479A1
公开(公告)日：2016-03-31
申请号：US14809972
申请日：2015-07-27
申请人： Fluidigm Corporation
发明人： Hany Nassef , Geoffrey Facer , Marc Unger
IPC分类号： G01N33/487 , G01N15/12 , G01N15/10
CPC分类号： C03C25/68 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N15/1031 , G01N15/12 , G01N27/00 , G01N27/60 , G01N33/48707 , G01N33/48721 , G01N2015/1087 , Y10T436/2575
摘要： The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.
摘要翻译： 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。
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公开(公告)号：US10131934B2
公开(公告)日：2018-11-20
申请号：US15805720
申请日：2017-11-07
申请人： Fluidigm Corporation
发明人： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
摘要： A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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公开(公告)号：US20180073051A1
公开(公告)日：2018-03-15
申请号：US15805720
申请日：2017-11-07
申请人： Fluidigm Corporation
发明人： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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