公开(公告)号：US20220282228A1

公开(公告)日：2022-09-08

申请号：US17608437

申请日：2020-05-01

Applicant: Amyris, Inc.

Inventor： Andrew P. KLEIN ,  Sean LUND ,  Gale WICHMANN ,  Irina KORYAKINA ,  Kyle HOGAN ,  Svetlana BORISOVA

IPC: C12N9/02 ,  C12P19/56

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of Ro.KAH. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides including rebaudioside A, rebaudioside D, and rebaudioside M.

公开(公告)号：US20220106619A1

公开(公告)日：2022-04-07

申请号：US17425634

申请日：2020-01-23

Applicant: AMYRIS, INC.

Inventor： Gale A. WICHMANN ,  Sean LUND ,  Joshua LERMAN ,  Hanxiao JIANG ,  Yi XIONG

IPC: C12P19/56 ,  C12N9/10 ,  C12N9/90 ,  C12N9/02 ,  C12N9/88 ,  C12P7/40 ,  C12P5/00

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US11219586B2

公开(公告)日：2022-01-11

申请号：US17375512

申请日：2021-07-14

Applicant: Amyris, Inc.

Inventor： Diva Chan ,  Ro Oteri ,  Ashlee Nunes

IPC: A61K31/01 ,  A61K31/015 ,  A61K31/045 ,  A61K8/34 ,  A61K8/31 ,  A61Q19/00

Abstract: Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.

公开(公告)号：US20210205191A1

公开(公告)日：2021-07-08

申请号：US17206588

申请日：2021-03-19

Applicant: Amyris, Inc.

Inventor： Diva CHAN ,  Ro OTERI ,  Ashlee NUNES

IPC: A61K8/34 ,  A61Q19/00 ,  A61K8/31

Abstract: Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.

公开(公告)号：US20210114953A1

公开(公告)日：2021-04-22

申请号：US16972291

申请日：2019-06-07

Applicant: Amyris, Inc.

Inventor： Karl Joseph FISHER ,  Frank Xavier WOOLARD

IPC: C07C1/32 ,  C12P5/02 ,  C07C209/60 ,  C07C17/093 ,  C07C67/10 ,  C07C29/128 ,  C07C315/00 ,  C07C315/04 ,  A61K39/39

Abstract: The present disclosure provides methods for preparing polyunsaturated hydrocarbons, such as E,E-farnesol, farnesyl acetate and squalene, by base catalyzed addition of a dialkylamine to a 3-methylene-1-alkene, such as farnesene. The present disclosure also provides compositions including one more farnesene derivatives prepared using the disclosed methods.

公开(公告)号：US10808015B2

公开(公告)日：2020-10-20

申请号：US15738555

申请日：2016-06-24

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. Chua ,  Hanxiao Jiang ,  Adam Leon Meadows

IPC: C07K14/395 ,  C12N1/16 ,  C12P19/62 ,  C12P5/02 ,  C12P7/64 ,  C12P21/00 ,  C12N15/52 ,  C12N15/63 ,  C12N15/65 ,  C12P21/02

Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

公开(公告)号：US20200270643A1

公开(公告)日：2020-08-27

申请号：US16733191

申请日：2020-01-02

Applicant: Amyris, Inc. ,  Total Marketing Services

Inventor： Penelope R. CHUA ,  Adam MEADOWS

IPC: C12P5/00 ,  C12N15/81 ,  C12N15/63 ,  C12N1/16

Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

公开(公告)号：US10597481B2

公开(公告)日：2020-03-24

申请号：US15548640

申请日：2016-02-05

Applicant: KURARAY CO., LTD. ,  AMYRIS, INC.

Inventor： Yosuke Uehara ,  Hiromitsu Sasaki ,  Daisuke Konishi ,  Masahiro Kato

IPC: C08F297/04 ,  C08F8/04 ,  C08F236/10 ,  C08F236/22 ,  C08F236/06 ,  C08F236/08 ,  D04H1/4282 ,  D04H3/007 ,  D01F6/28 ,  B29C43/02 ,  C08J5/18 ,  B32B27/00 ,  C09J153/02 ,  C08L53/02 ,  C08L25/10 ,  C08L23/00 ,  B32B27/30

Abstract: A hydrogenated block copolymer prepared by hydrogenating a block copolymer that contains a polymer block (A) containing a structural unit derived from an aromatic vinyl compound, a polymer block (B) containing a structural unit derived from farnesene, and a polymer block (C) containing a structural unit derived from a conjugated diene except farnesene, wherein the block copolymer contains at least two above polymer blocks (A), at least one above polymer block (B) and at least one above polymer block (C), and wherein at least one above polymer block (B) is at the terminal, and the hydrogenation rate of the carbon-carbon double bond in the polymer block (B) and the polymer block (C) is 50 mol % or more.

公开(公告)号：US10550248B2

公开(公告)日：2020-02-04

申请号：US14913638

申请日：2014-08-11

Applicant: KURARAY CO., LTD. ,  AMYRIS, INC.

Inventor： Daisuke Koda ,  Kei Hirata

IPC: C08L9/06

Abstract: The present invention provides a rubber composition capable of producing a rubber molded article that is excellent in rolling resistance performance, steering stability, mechanical strength and abrasion resistance, and a tire obtained by using the rubber composition in at least a part thereof. The present invention relates to a rubber composition including (A) a rubber component including at least one rubber selected from the group consisting of a synthetic rubber and a natural rubber, (B) silica and (C) a modified polymer of farnesene obtained by introducing a functional group into a polymer of farnesene, in which a content of the silica (B) in the rubber composition is from 20 to 150 parts by mass on the basis of 100 parts by mass of the rubber component (A), and a content of the modified farnesene polymer (C) in the rubber composition is from 2 to 10 parts by mass on the basis of 100 parts by mass of the silica (B).

公开(公告)号：US20190382773A1

公开(公告)日：2019-12-19

申请号：US15771888

申请日：2016-10-28

Applicant: AMYRIS, INC.

Inventor： Andrew MAIN ,  Grzegorz WOJCIECHOWSKI ,  Yue YANG ,  Lishan ZHAO

IPC: C12N15/52 ,  C12P5/00

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nude is acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.