利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

1289 条记录 (耗时 0.046 秒)

    Compositions and methods for making and using laminin nanofibers
    113.
    发明授权
    Compositions and methods for making and using laminin nanofibers 有权
    制备和使用层粘连蛋白纳米纤维的组合物和方法

    公开(公告)号：US08728817B2

    公开(公告)日：2014-05-20

    申请号：US13045095

    申请日：2011-03-10

    申请人： Roy Clinton Ogle ,  Edward A. Botchwey, III ,  Rebekah A. Neal

    发明人： Roy Clinton Ogle ,  Edward A. Botchwey, III ,  Rebekah A. Neal

    IPC分类号： C12N5/0793 ,  C07K14/00

    CPC分类号： B82Y5/00 ,  C07K14/78 ,  C08L89/00 ,  C12N5/0619 ,  C12N2533/00 ,  C08L67/02 ,  C08L67/06 ,  C08L67/04 ,  C08L31/04

    摘要： The present invention provides methodologies and parameters for fabrication of the hybrid biomaterial by blending pure laminin or complex extracts of tissues containing laminin with biopolymers such as polycaprolactone (PCL), polylactic/polyglycolic acid copolymer (PLGA) or Polydioxanone (PDO) in fluoroalcohols (HFP, TFA), fabrication of substrates and scaffolds and devices from the hybrid biomaterial in forms such as films, nanofibers by electrospinning or microspheres, and the biological or biomedical use of the material or devices derived from it.

    摘要翻译： 本发明通过将含有层粘连蛋白的组织的纯层粘连蛋白或复合提取物与生物聚合物如聚己内酯（PCL），聚乳酸/聚乙醇酸共聚物（PLGA）或聚二恶烷酮（PDO）在氟代醇（HFP）中共混来提供混合生物材料的制备方法和参数 ，TFA），通过静电纺丝或微球形式从诸如膜，纳米纤维的形式从混合生物材料制备底物和支架和装置，以及从其衍生的材料或器件的生物或生物医学用途。

    METHODS TO TREAT NERVOUS SYSTEM CONDITIONS
    114.
    发明申请
    METHODS TO TREAT NERVOUS SYSTEM CONDITIONS 审中-公开
    治疗神经系统病症的方法

    公开(公告)号：US20140079802A1

    公开(公告)日：2014-03-20

    申请号：US14018849

    申请日：2013-09-05

    申请人： Keith Leonard March ,  Brian H. Johnstone ,  Yansheng Du

    发明人： Keith Leonard March ,  Brian H. Johnstone ,  Yansheng Du

    IPC分类号： A61K35/12

    CPC分类号： C12N5/0667 ,  A61K35/28 ,  A61K35/35 ,  C12N5/06 ,  C12N5/0619 ,  C12N2502/00 ,  C12N2502/1305 ,  C12N2502/1382

    摘要： Methods to treat nervous system conditions. In at least one embodiment of a method of treating a mammalian patient having a neuronal injury or insult of the present disclosure, the method comprises the step of administering a therapeutically-effective dose of a stem cell conditioned medium to a mammalian patient, the stem cell conditioned medium comprising a cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult.

    摘要翻译： 治疗神经系统疾病的方法 在治疗哺乳动物患者具有本公开的神经元损伤或侮辱的方法的至少一个实施方案中，该方法包括向哺乳动物患者施用治疗有效剂量的干细胞条件培养基的步骤，所述干细胞 条件培养基，其包含含有至少一种能够发挥有效神经保护作用以治疗哺乳动物神经损伤或损伤的因子的细胞培养上清液。

    Methods of neural conversion of human embryonic stem cells
    116.
    发明授权
    Methods of neural conversion of human embryonic stem cells 有权
    人胚胎干细胞神经转换方法

    公开(公告)号：US08642334B2

    公开(公告)日：2014-02-04

    申请号：US13201137

    申请日：2010-02-17

    申请人： Stuart Chambers ,  Lorenz Studer

    发明人： Stuart Chambers ,  Lorenz Studer

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0619 ,  C12N2500/90 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2506/45

    摘要： The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning.

    摘要翻译： 本发明一般涉及干细胞的细胞生物学领域，更具体地涉及多能或多潜能干细胞的定向分化，包括人胚胎干细胞（hESC），体细胞干细胞和诱导的人多能干细胞（hiPSC），其使用 新颖的文化条件。 具体地，提供了用于获得神经组织，底板细胞和细胞凋亡的方法，包括在hESC中诱导神经平板发育以获得中脑多巴胺（DA）神经元，运动神经元和感觉神经元。 此外，使用本发明的方法获得的神经平板组织预期用于与其他组织的共培养物作为用于移动分化途径的诱导剂，即图案化。

    NEURAL PROGENITOR CELLS DERIVED FROM EMBRYONIC STEM CELLS
    119.
    发明申请
    NEURAL PROGENITOR CELLS DERIVED FROM EMBRYONIC STEM CELLS 审中-公开
    从胚胎干细胞衍生的神经前体细胞

    公开(公告)号：US20130330821A1

    公开(公告)日：2013-12-12

    申请号：US13874507

    申请日：2013-05-01

    申请人： ES Cell International PTE Ltd.

    发明人： BENJAMIN EITHAN REUBINOFF ,  MARTIN Federick PERA ,  TAMIR BEN-HUR

    IPC分类号： C12N5/0797

    CPC分类号： C12N5/0623 ,  A61K35/12 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/91 ,  C12N2502/13 ,  C12N2506/02

    摘要： The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. This invention provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and cells that are generated along the pathway of neural differentiation may be used for: the study of the cellular and molecular biology of human neural development, discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, drug discovery and the development of screening assays for teratogenic, toxic and neuroprotective effects.

    摘要翻译： 本发明涉及未分化的人类胚胎干细胞，培养和繁殖方法以及分化细胞的产生。 特别地，它涉及能够在体外产生体细胞分化细胞的人ES细胞的产生，以及确定的祖细胞，例如能够产生包括神经细胞和/或胶质细胞的成熟体细胞的神经祖细胞及其用途 。 本发明提供了产生ES细胞向神经谱系的受控分化的体外和体内模型的方法。 沿着神经分化途径产生的模型和细胞可用于：人类神经发育的细胞和分子生物学的研究，发现基因，生长因子和在神经分化中发挥作用的分化因子， 再生，药物发现和开发用于致畸，毒性和神经保护作用的筛选试验。

    Cultures of GFAP+ nestin+ cells that differentiate to neurons
    120.
    发明授权
    Cultures of GFAP+ nestin+ cells that differentiate to neurons 有权
    培养分化为神经元的GFAP + nestin +细胞

    公开(公告)号：US08501467B2

    公开(公告)日：2013-08-06

    申请号：US12646557

    申请日：2009-12-23

    申请人： Lars Wahlberg ,  Kenneth Campbell ,  Charlotta Skogh ,  Cecilia Eriksson ,  Klas Wictorin

    发明人： Lars Wahlberg ,  Kenneth Campbell ,  Charlotta Skogh ,  Cecilia Eriksson ,  Klas Wictorin

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0622 ,  A61K35/12 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2500/90 ,  C12N2501/11

    摘要： Cultures of cells immunoreactive for glial fibrillary acidic protein (GFAP), as well as for the intermediate filament marker nestin were grown in a medium including epidermal growth factor (EGF) and serum. The cultured cells had the morphology of astroglial cells. The cells can be proliferated in adherent or suspension cultures. Depending on the culture conditions, the cells can be induced to differentiate to neurons or glial cells. The cultures can be expanded over a large number of passages during several months, and survive, express an astroglial phenotype and integrate well after transplantation into both neonatal and adult rat forebrain.

    摘要翻译： 对胶质纤维酸性蛋白（GFAP）以及中间丝标记物巢蛋白免疫反应性细胞的培养物在包括表皮生长因子（EGF）和血清的培养基中生长。 培养的细胞具有星形胶质细胞的形态。 细胞可以在粘附或悬浮培养物中增殖。 根据培养条件，可诱导细胞分化为神经元或神经胶质细胞。 文化可以在几个月内扩大大量的传代，并且生存，表达一种星形胶质细胞表型，并在移植后并入新生大鼠和成年大鼠前脑中。