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    VARIANT POLYPEPTIDES AND METHODS OF MAKING
    111.
    发明申请
    VARIANT POLYPEPTIDES AND METHODS OF MAKING 有权
    各种多样性和制备方法

    公开(公告)号：US20080070287A1

    公开(公告)日：2008-03-20

    申请号：US11852274

    申请日：2007-09-07

    申请人： Jorn Soe ,  Jorn Mikkelson ,  Arno Kreij

    发明人： Jorn Soe ,  Jorn Mikkelson ,  Arno Kreij

    IPC分类号： C12N5/04 ,  A21D13/04 ,  A23L1/32 ,  C12P7/64 ,  C12N9/10 ,  C12N15/00

    CPC分类号： C12P7/6463 ,  C12P7/6454

    摘要： The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and/or phospholipid and/or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing a Bacillus licheniformis cell; (vi) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and/or clipping, i.e., to remove a signal peptide. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.

    摘要翻译： 本发明涉及生产变体脂质酰基转移酶的方法，包括以下步骤：（i）选择脂质酰基转移酶的亲本酶; （ii）修饰一个或多个氨基酸以产生变体脂质酰基转移酶; （iii）测试变体脂质酰基转移酶对半乳糖脂和/或磷脂和/或甘油三酯底物的活性; （iv）与亲本酶相比，选择具有增强的对半乳糖脂的活性的变体酶; （v）提供地衣芽孢杆菌细胞; （vi）用编码所述变体脂质酰基转移酶的异源核苷酸序列转化地衣芽孢杆菌细胞; 和（iii）在启动子序列控制下的细胞中表达所述变体脂质酰基转移酶。 变体脂质酰基转移酶可经历翻译后修饰，截短和/或剪切，即去除信号肽。 此外，本发明还涉及地衣芽孢杆菌表达脂质酰基转移酶，包含异源脂质酰基转移酶的地衣芽孢杆菌宿主细胞的使用和包含编码脂质酰基转移酶的核苷酸序列的载体，所述核苷酸序列可操作地连接到与B同源的启动子序列 地衣芽孢杆菌

    Method for producing symmetric triglycerides
    113.
    发明申请
    Method for producing symmetric triglycerides 有权
    生产对称甘油三酯的方法

    公开(公告)号：US20060115882A1

    公开(公告)日：2006-06-01

    申请号：US11326436

    申请日：2006-01-06

    申请人： Satoshi Negishi ,  Yuri Arai ,  Shin Arimoto ,  Hidetaka Uehara

    发明人： Satoshi Negishi ,  Yuri Arai ,  Shin Arimoto ,  Hidetaka Uehara

    IPC分类号： C12P7/64

    CPC分类号： A23D9/00 ,  C11C3/10 ,  C12P7/6454 ,  C12P7/6472

    摘要： A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride composed of a medium chain fatty acid and a long chain fatty acid as the constituting fatty acids in the step of the first reaction; transesterifying the reaction product with an alcohol monoester of the medium chain fatty acid in the presence of an sn-1,3-position specific enzyme in the step of the second reaction and then taking the alcohol monoester of the medium chain fatty acid and the alcohol monoester of the long chain fatty acid from the reaction product obtained in the step of the second reaction to obtain the symmetric triglyceride composed of the medium chain fatty acids at the sn-1, 3 positions and the long chain fatty acid at the sn-2 position. According to this method, highly pure symmetric triglycerides comprising a medium-chain fatty acid at sn-1 and 3 positions and a long chain fatty acid at sn-2 position can be industrially efficiently produced.

    摘要翻译： 一种对称甘油三酯的制造方法，其特征在于，在酶或化学催化剂存在下使中链脂肪酸甘油三酯与长链脂肪酸甘油三酯反应，进行无规酯交换反应，得到含有 在第一反应步骤中由构成脂肪酸的中链脂肪酸和长链脂肪酸组成的甘油三酸酯; 在第二反应步骤中，在sn-1,3位特异性酶存在下，用中链脂肪酸的醇单酯将反应产物进行酯交换，然后取中链脂肪酸和醇的醇单酯 在第二反应步骤中获得的反应产物中的长链脂肪酸的单酯，得到由sn-1,3位置的中链脂肪酸组成的对称甘油三酯，sn-2处的长链脂肪酸 位置。 根据该方法，可以在工业上有效地制造包含sn-1和3位的中链脂肪酸和sn-2位的长链脂肪酸的高纯度对称甘油三酯。

    Method of producing diacylglycerides
    114.
    发明申请
    Method of producing diacylglycerides 审中-公开
    制备二酰基甘油酯的方法

    公开(公告)号：US20060084153A1

    公开(公告)日：2006-04-20

    申请号：US10966489

    申请日：2004-10-15

    申请人： Wuli Bao ,  Mark Matlock ,  Leif Solheim

    发明人： Wuli Bao ,  Mark Matlock ,  Leif Solheim

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6454 ,  C11C3/04 ,  C11C3/10

    摘要： Embodiments of the present invention are directed to a method of producing diacylglycerides comprising mixing one or more fatty acid esters of glycerol and at least one enzyme selected from a lipase and an endo-lipase and transesterifying at least a portion of the one or more fatty acid esters of glycerol to produce diacylglycerides. The enzyme may be a free enzyme or the enzyme may be immobilized. The fatty acid esters of glycerol may comprise at least one of monoacylglycerides, diacylglycerides, and triacylglycerides. Additionally, embodiments of the method of the present invention may comprise mixing at least one of a fatty acid and a fatty acid lower alcohol ester with the one or more fatty acid esters of glycerol and the enzyme. Additional embodiments may comprise mixing glycerol with the one or more fatty acid esters of glycerol and the enzyme.

    摘要翻译： 本发明的实施方案涉及一种生产二酰基甘油酯的方法，其包括将一种或多种甘油脂肪酸酯和至少一种选自脂肪酶和内切脂肪酶的酶混合并酯交换至少一部分一种或多种脂肪酸 甘油的酯产生二酰基甘油酯。 酶可以是游离酶，或者可以固定酶。 甘油的脂肪酸酯可以包含单酰基甘油酯，二酰基甘油酯和三酰基甘油酯中的至少一种。 另外，本发明方法的实施方案可以包括将脂肪酸和脂肪酸低级醇酯中的至少一种与甘油和酶的一种或多种脂肪酸酯混合。 另外的实施方案可以包括将甘油与甘油和酶的一种或多种脂肪酸酯混合。

    Astaxanthin medium-chain fatty acid ester, process for producing the same and composition containing the ester
    115.
    发明申请
    Astaxanthin medium-chain fatty acid ester, process for producing the same and composition containing the ester 审中-公开
    虾青素中链脂肪酸酯，其制备方法和含有酯的组合物

    公开(公告)号：US20050228188A1

    公开(公告)日：2005-10-13

    申请号：US10511829

    申请日：2003-04-28

    申请人： Motoo Sumida ,  Masahiro Nakao ,  Namino Tomimori ,  Koshi Namikawa ,  Harukazu Fukami

    发明人： Motoo Sumida ,  Masahiro Nakao ,  Namino Tomimori ,  Koshi Namikawa ,  Harukazu Fukami

    IPC分类号： A23L1/29 ,  A23L1/30 ,  A61K8/37 ,  A61Q1/06 ,  C07C403/24 ,  C12P7/62 ,  C12P7/64 ,  C12P23/00 ,  C07C57/26

    CPC分类号： C07C403/24 ,  A23L33/10 ,  A23L33/115 ,  A61K8/37 ,  A61Q1/06 ,  C12P7/62 ,  C12P7/6436 ,  C12P7/6454 ,  C12P23/00

    摘要： The present invention provides an astaxanthin medium-chain fatty acid ester, which is expected to be applied in the fields of food, cosmetics and pharmaceuticals, and has higher digestibility and tissue penetration than long chain fatty acid ester form astaxanthins. When the synthesis of an astaxanthin medium-chain fatty acid ester is attempted using the catalytic action of lipase by conventional methods, ester cannot be formed. However, according to the present invention, a composition comprising an astaxanthin medium-chain fatty acid monoester and/or diester is produced by adding a certain amount of water into a reaction system and reacting an astaxanthin or a long chain fatty acid ester thereof with a medium-chain fatty acid, a triglyceride thereof or a suitable ester. Moreover, these monoesters and/or diesters are isolated, as necessary. The present invention further provides food or cosmetics comprising the composition of the present invention.

    摘要翻译： 本发明提供了一种预期用于食品，化妆品和药物领域的虾青素中链脂肪酸酯，并且比长链脂肪酸酯形式的虾青素具有更高的消化率和组织穿透性。 当通过常规方法尝试使用脂肪酶的催化作用来合成虾青素中链脂肪酸酯时，不能形成酯。 然而，根据本发明，通过向反应体系中加入一定量的水并使虾青素或其长链脂肪酸酯与一种虾青素或其长链脂肪酸酯反应而制备包含虾青素中链脂肪酸单酯和/或二酯的组合物 中链脂肪酸，其甘油三酯或合适的酯。 此外，根据需要分离这些单酯和/或二酯。 本发明还提供包含本发明组合物的食品或化妆品。

    Method for enzymatic splitting of oils and fats
    116.
    发明授权
    Method for enzymatic splitting of oils and fats 失效
    油脂分解方法

    公开(公告)号：US06933139B2

    公开(公告)日：2005-08-23

    申请号：US10170608

    申请日：2002-06-11

    申请人： Karlheinz Brunner ,  Rainer Frische ,  Dirk Kilian

    发明人： Karlheinz Brunner ,  Rainer Frische ,  Dirk Kilian

    IPC分类号： C12M1/40 ,  B01D17/038 ,  C11C1/00 ,  C11C1/04 ,  C11C1/06 ,  C11C3/00 ,  C11C3/10 ,  C12M1/00 ,  C12M1/33 ,  C12P7/20 ,  C12P7/40 ,  C12P7/62 ,  C12P7/64

    CPC分类号： C12P7/6418 ,  C11C1/007 ,  C11C1/045 ,  C11C3/003 ,  C11C3/10 ,  C12P7/20 ,  C12P7/62 ,  C12P7/6445 ,  C12P7/6454

    摘要： Method for the enzymatic splitting of oils and fats for obtaining fatty acids and glycerol by using lipases being added to a mixture containing an oil or fat and water, wherein the splitting reaction is performed only up to a splitting degree at which slowing-down of the splitting reaction is still below a preset value using discontinuously operated loop reactors, wherein the fatty acids to be obtained are separated from the reaction mixture that is only partially split, by first separating an aqueous glycerol-containing phase from a partially split organic phase containing split fatty acids, in a self-cleaning centrifugal separator and, afterwards, the fatty acids are separated from the partially split organic phase and the residue of the organic phase freed from the free fatty acids is fed back into the splitting process.

    摘要翻译： 通过使用脂肪酶加入到含有油或脂肪和水的混合物中来分解油和脂肪以获得脂肪酸和甘油的方法，其中分裂反应仅进行到分解程度，其中缓解 通过使用不连续操作的环流反应器将分解反应仍低于预设值，其中将待获得的脂肪酸与仅部分裂解的反应混合物分离，首先将含水甘油相与含裂解的有机相分离 在自清洁的离心分离器中，然后将脂肪酸与部分裂解的有机相分离，并将游离脂肪酸脱除的有机相的残余物反馈到分离过程中。

    Method for producing fats or oils
    117.
    发明申请
    Method for producing fats or oils 有权
    生产脂肪或油的方法

    公开(公告)号：US20050014237A1

    公开(公告)日：2005-01-20

    申请号：US10875532

    申请日：2004-06-25

    申请人： Inmok Lee

    发明人： Inmok Lee

    IPC分类号： C11C3/10 ,  C12P7/62 ,  C12P7/64 ,  C12P17/02

    CPC分类号： C11C3/10 ,  C12P7/62 ,  C12P7/6454 ,  Y10S435/80 ,  Y10S435/962

    摘要： The present invention is directed to improving productivity of an enzymatic method for making esterified, transesterified or interesterified products. Specifically, a method that can greatly improve the productivity of enzymatic transesterification or esterification by deodorization alone, or by deodorization and purification of the initial substrate to extend the useful life of the enzyme is disclosed.

    摘要翻译： 本发明旨在提高用于制备酯化，酯交换或酯交换产物的酶法的生产率。 具体地说，公开了一种可以通过单独除臭或通过除臭和纯化初始底物来大大提高酶酯交换或酯化的生产力以延长酶的使用寿命的方法。

    Preparation process of diglyceride
    120.
    发明授权
    Preparation process of diglyceride 有权
    甘油二酯的制备方法

    公开(公告)号：US6361980B2

    公开(公告)日：2002-03-26

    申请号：US72557100

    申请日：2000-11-30

    申请人： KAO CORP

    发明人： SUGIURA MASAKATSU ,  YAMAGUCHI HIROAKI ,  YAMADA NAOTO

    IPC分类号： C11C3/06 ,  C12P7/64 ,  C07C51/00 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/62

    CPC分类号： C12P7/6454 ,  C12P7/6472

    摘要： The present invention provides a process for preparing a diglyceride, which includes:in an enzyme-packed tower which includes an immobilized lipase preparation, carrying out an esterification reaction between:(1) an acyl group donor selected from the group including a fatty acid, a lower alcohol ester thereof, and a mixture thereof; and(2) an acyl group acceptor selected from the group including glycerol, a monoglyceride, and a mixture thereof;to obtain a reaction fluid from the enzyme-packed tower;reducing a water content or a lower alcohol content in the reaction fluid; andsubsequent to the reducing, recirculating the reaction fluid to the enzyme-packed tower, wherein a residence time of the reaction fluid in the enzyme-packed tower is 120 seconds or less;to obtain a diglyceride. According to the present invention, a high-purity glyceride can be provided at a high yield in a short period of time.

    摘要翻译： 本发明提供了一种制备甘油二酯的方法，其包括：在包含固定化脂肪酶制剂的酶填充塔中进行以下酯化反应：（1）选自脂肪酸， 其低级醇酯及其混合物; 和（2）选自包括甘油，单甘油酯及其混合物的酰基受体;从酶填充塔中获得反应流体;降低反应流体中的水含量或低醇含量; 并且随后还原，将反应流体再循环到酶填充塔中，其中反应流体在酶填充塔中的停留时间为120秒或更少;以获得甘油二酯。 根据本发明，可以在短时间内以高产率提供高纯度甘油酯。