公开(公告)号：US09364492B2

公开(公告)日：2016-06-14

申请号：US12523065

申请日：2008-01-18

申请人： Misa Ochiai ,  Harukazu Fukami ,  Masahiro Nakao ,  Akio Noguchi

发明人： Misa Ochiai ,  Harukazu Fukami ,  Masahiro Nakao ,  Akio Noguchi

IPC分类号： C07H15/203 ,  C07H17/065 ,  A23L2/52 ,  A61K31/7048 ,  A23L1/03 ,  A23L1/30 ,  A61K8/60 ,  A61K31/7034 ,  A61Q19/00 ,  C12P19/14

CPC分类号： A61K31/7048 ,  A23L2/52 ,  A23L29/06 ,  A23L33/105 ,  A23V2002/00 ,  A61K8/602 ,  A61K31/7034 ,  A61Q19/00 ,  C07H15/203 ,  C07H17/065 ,  C12P19/14 ,  A23V2250/21166 ,  A23V2250/208

摘要： The present invention provides a method for preparing a glycoside of a flavonoid compound, which comprises the step of treating flavonoid and a glycosyl donor with an enzymatic agent having glycosylation activity and being derived from the genus Trichoderma (preferably Trichoderma viride or Trichoderma reesei). Such a flavonoid compound includes a catechin compound or a methylated derivative thereof, and the glycosyl donor includes a carbohydrate containing a maltotriose residue (preferably maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, dextrin, γ-cyclodextrin or soluble starch). Glycosides obtained by the present invention have higher water solubility, improved taste, and increased stability. The present invention also provides novel glycosides of catechin compounds, which are obtained by the method of the present invention.

摘要翻译： 本发明提供了一种制备类黄酮化合物的糖苷的方法，其包括用具有糖基化活性的酶促处理类黄酮和糖基供体的步骤，其衍生自木霉属（优选绿色木霉或里氏木霉）。 这种类黄酮化合物包括儿茶素化合物或其甲基化衍生物，糖基供体包括含有麦芽三糖残基（优选麦芽三糖，麦芽四糖，麦芽五糖，麦芽六糖，麦芽七糖，糊精，γ-环糊精或可溶性淀粉）的碳水化合物。 通过本发明获得的糖苷具有更高的水溶性，改善的味道和增加的稳定性。 本发明还提供了通过本发明的方法获得的新颖的儿茶素化合物的糖苷。

公开(公告)号：US08236854B2

公开(公告)日：2012-08-07

申请号：US12754084

申请日：2010-04-05

申请人： Kengo Akimoto ,  Harukazu Fukami ,  Toshinao Goda

发明人： Kengo Akimoto ,  Harukazu Fukami ,  Toshinao Goda

IPC分类号： A01N37/02

CPC分类号： C12P7/6472 ,  A23D9/00 ,  A23L33/115 ,  A23V2002/00 ,  A61K31/231 ,  A61K31/232 ,  C11C3/08 ,  A23V2250/5112 ,  A23V2250/1862

摘要： A lipid-improving agent containing a triglyceride(s) where a poly-unsaturated fatty acid is bonded to 2-position of a triglyceride(s).

摘要翻译： 含有甘油三酯的脂质改进剂，其中多不饱和脂肪酸与甘油三酯的2-位结合。

公开(公告)号：US07893232B2

公开(公告)日：2011-02-22

申请号：US12096574

申请日：2006-12-08

申请人： Masahiro Nakao ,  Masaki Kanamori ,  Harukazu Fukami ,  Hiroaki Kasai ,  Misa Ochiai

发明人： Masahiro Nakao ,  Masaki Kanamori ,  Harukazu Fukami ,  Hiroaki Kasai ,  Misa Ochiai

IPC分类号： C07H21/02 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/20 ,  C12P7/6409 ,  C12P7/6436 ,  C12R1/01

摘要： The present invention provides a novel lipase with a molecular weight of about 32 kDa, which is produced by a strain belonging to the genus Tetrasphaera, as well as a gene encoding the same. This lipase has the ability to recognize a medium-chain fatty acid as a substrate. The present invention also provides a novel lipase with a molecular weight of about 40 kDa, which is produced by a strain belonging to the genus Tetrasphaera and has the ability to recognize both a medium-chain fatty acid and a long-chain fatty acid as substrates, as well as a polynucleotide encoding the same. The present invention further provides Tetrasphaera sp. strain NITE P-154. The lipase of the present invention can be used as an immobilized enzyme and is useful in fields such as production of digestants and/or flavorings, production of clinical laboratory reagents, detergent enzymes and/or fats, as well as production of optically active intermediates for agricultural chemicals and pharmaceutical preparations.

摘要翻译： 本发明提供了一种分子量约32kDa的新型脂肪酶，其由属于四联属属的菌株产生，以及编码该脂肪酶的基因。 该脂肪酶具有识别中链脂肪酸作为底物的能力。 本发明还提供一种分子量约40kDa的新型脂肪酶，其由属于四孢属属的菌株产生，并且具有将中链脂肪酸和长链脂肪酸同时识别为底物的能力 ，以及编码该多核苷酸的多核苷酸。 本发明进一步提供了Tetrasphaera sp。 菌株NITE P-154。 本发明的脂肪酶可以用作固定化酶，可用于生产消化剂和/或调味剂，生产临床实验室试剂，洗涤剂和/或脂肪等领域，以及生产光学活性中间体 农药和药物制剂。

公开(公告)号：US20100273154A1

公开(公告)日：2010-10-28

申请号：US12523068

申请日：2008-01-18

申请人： Misa Ochiai ,  Harukazu Fukami ,  Masahiro Nakao ,  Akio Noguchi

发明人： Misa Ochiai ,  Harukazu Fukami ,  Masahiro Nakao ,  Akio Noguchi

IPC分类号： C12Q1/68 ,  C12N11/08 ,  C12N9/10 ,  C12N5/10 ,  C12N15/63 ,  C07H21/04

CPC分类号： C12N9/1048

摘要： The present invention provides novel glycosyltransferase proteins produced by ascomycetous filamentous fungi (preferably species belonging to the genus Trichoderma, more preferably Trichoderma viride), as well as genes encoding the same. Among novel enzyme proteins provided by the present invention, particularly preferred is an enzyme protein obtained from the culture supernatant of Trichoderma viride strain IAM5141. The novel enzymes of the present invention allow glycosylation of flavonoid compounds to thereby improve their water solubility.

摘要翻译： 本发明提供了由Ascomycetoto丝状真菌（优选属于木霉属，更优选绿色木霉）的物质以及编码它们的基因产生的新的糖基转移酶蛋白质。 在本发明提供的新型酶蛋白中，特别优选从绿色木霉菌株IAM5141的培养物上清液获得的酶蛋白质。 本发明的新型酶可以使类黄酮化合物进行糖基化从而提高其水溶性。

公开(公告)号：US07781409B2

公开(公告)日：2010-08-24

申请号：US10561916

申请日：2004-06-25

申请人： Mitsuru Maeda ,  Masahiro Nakao ,  Harukazu Fukami

发明人： Mitsuru Maeda ,  Masahiro Nakao ,  Harukazu Fukami

IPC分类号： A61K31/047 ,  A61K36/00 ,  C12N1/00

CPC分类号： A61Q19/02 ,  A61K8/676 ,  A61K8/99 ,  A61K31/704 ,  A61K2800/522 ,  A61K2800/70 ,  A61K2800/75 ,  A61K2800/782 ,  A61Q19/00 ,  Y10S435/913 ,  Y10S435/914 ,  Y10S435/918

摘要： A composition for external use, comprising 2-O-(β-D-glucopyranosyl)ascorbic acid represented by the formula (I): or a salt or ester thereof which is safe to the human body, and a koji mold or a processed koji. The composition for external use is excellent in skin permeability, containing an ascorbic acid derivative which is excellent in stability, utilized persistently in the living body, and strong in antioxidant activity, and has little skin irritation.

摘要翻译： 一种外用组合物，其包含由式（I）表示的2-O-（＆bgr-D-吡喃葡萄糖基）抗坏血酸：或其对人体安全的盐或酯，以及曲霉或经加工的 曲子 外用组合物的皮肤透过性优异，含有稳定性优异的抗坏血酸衍生物，在生物体中持续使用，抗氧化活性强，皮肤刺激性小。

公开(公告)号：US20080070983A1

公开(公告)日：2008-03-20

申请号：US11898121

申请日：2007-09-10

申请人： Mitsuru Maeda ,  Masahiro Nakao ,  Harukazu Fukami

发明人： Mitsuru Maeda ,  Masahiro Nakao ,  Harukazu Fukami

IPC分类号： A61K31/375 ,  A61P43/00

CPC分类号： A61K8/676 ,  A23L33/105 ,  A23L33/15 ,  A23V2002/00 ,  A61K8/602 ,  A61K31/375 ,  A61K2800/52 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/02 ,  C07H1/08 ,  C07H17/04 ,  C12P19/60 ,  A23V2250/708

摘要： The present invention provides a novel ascorbic acid derivative as a provitamin C with improved stability in the body and prolonged life in the body compared to conventionally known 2-O-(α-D-glucopyranosyl)ascorbic acid. The composition comprising the novel compound 2-O-(β-D-glucopyranosyl)ascorbic acid has been extracted from plants such as from Ningxia Lycium barbarum L. and/or Lycium chinense Mill. The compositions comprising 2-O-(β-D-glucopyranosyl)ascorbic acid may be enzymatically synthesized using β-D-glucosyltransferase. Pure 2-O-(β-D-glucopyranosyl)ascorbic acid may be produced from such compositions. Alternatively, 2-O-(β-D-glucopyranosyl)ascorbic acid may be produced by chemical synthesis. The 2-O-(β-D-glucopyranosyl)ascorbic acid results in higher stability and a prolonged life of vitamin C when ingested in the body compared to the corresponding α-D-glucopyranosyl derivative, and is therefore highly suitable as a provitamin C to be used in cosmetics and foods.

摘要翻译： 与常规已知的2-O-（α-D-吡喃葡萄糖基）抗坏血酸相比，本发明提供了一种新型抗坏血酸衍生物作为维生素C，其具有改善的身体稳定性和延长的身体寿命。 含有新化合物2-O-（β-D-吡喃葡萄糖基）抗坏血酸的组合物已经从宁夏枸杞子和/或枸杞子等植物中提取出来。 包含2-O-（β-D-吡喃葡萄糖基）抗坏血酸的组合物可以使用β-D-葡糖基转移酶酶促合成。 可以从这样的组合物制备纯的2-O-（β-D-吡喃葡萄糖基）抗坏血酸。 或者，可通过化学合成制备2-O-（β-D-吡喃葡萄糖基）抗坏血酸。 与相应的α-D-吡喃葡萄糖基衍生物相比，2-O-（β-D-吡喃葡萄糖基）抗坏血酸产生更高的稳定性和摄取维生素C的寿命，因此非常适合作为维生素C 用于化妆品和食品。

公开(公告)号：US20070269794A1

公开(公告)日：2007-11-22

申请号：US11660962

申请日：2005-08-24

申请人： Mitsuyoshi Ueda ,  Masahiro Nakao ,  Maki Asami ,  Misa Ochiai ,  Harukazu Fukami

发明人： Mitsuyoshi Ueda ,  Masahiro Nakao ,  Maki Asami ,  Misa Ochiai ,  Harukazu Fukami

IPC分类号： C12Q1/25 ,  C12M1/34

CPC分类号： C12Q1/34 ,  C12Q1/04

摘要： A method and apparatus for screening a microorganism or protein having a useful function by using a micro-chamber array. Using the screening method and screening apparatus in accordance with the present invention, a target protein having an enzymatic activity or a microorganism producing such a protein is screened from a sample serving as a screening object by using a base plate having a plurality of micro-chambers arranged in the form of an array. With this method and apparatus, a protein having an enzymatic activity or a microorganism producing such a protein that is present in a micro-chamber is detected by detecting a fluorescence produced by an enzyme reaction of the protein having an enzymatic activity and a fluorescence substrate.

摘要翻译： 一种通过使用微室阵列筛选具有有用功能的微生物或蛋白质的方法和装置。 使用根据本发明的筛选方法和筛选装置，通过使用具有多个微室的基板，从用作筛选对象的样品中筛选具有酶活性的靶蛋白或产生这种蛋白质的微生物 以阵列的形式排列。 利用该方法和装置，通过检测由具有酶活性的蛋白质和荧光底物的酶反应产生的荧光来检测具有酶活性的蛋白质或产生存在于微室中的蛋白质的微生物。

公开(公告)号：US07057036B2

公开(公告)日：2006-06-06

申请号：US10615836

申请日：2003-07-10

申请人： Akira Mizuno ,  Makoto Shibata ,  Tomoe Kamei ,  Harukazu Fukami ,  Norio Inomata

发明人： Akira Mizuno ,  Makoto Shibata ,  Tomoe Kamei ,  Harukazu Fukami ,  Norio Inomata

IPC分类号： C07D267/02 ,  C07D513/00 ,  C07D207/00

CPC分类号： C07D513/04 ,  C07D207/36

摘要： A pyrrolesulfonamide derivative having the following formula (I): wherein the ring P represented by is a pyrrole ring having the following structure: wherein R represents alkyl, cycloalkyl, cycloalkyl-alkyl or aralkyl; the dashed line indicates the presence or absence of a bond; and, when the bond is present, Z2 is not present and Z1 represents H but, when the bond is absent, Z1 represents H and Z2 represents OH or Z1 and Z2 are combined together to represent O or a group NOR1, in which R1 represents H, or alkyl, aralkyl or aryl; l stands for 0 or 1; A represents alkylene, alkenylene or alkynylene; and Y represents a group in which W represents CH, C═ or N; m stands for 0 or 1 when W is CH or N, or m stands for 1 when W is C═; B represents a specific divalent group; E1 and E2 each independently represents H or lower alkyl; and D represents an aromatic hydrocarbon group or heterocyclic group. The compound (I) has strong serotonin-2 receptor antagonistic action and low toxicity and less side effects, and is useful as a therapeutic for circulatory diseases such as ischemic heart diseases, cerebrovascular disturbances and peripheral circulatory disturbances.

摘要翻译： 具有下式（I）的吡咯磺酰胺衍生物：其中由代表的环P是具有以下结构的吡咯环：其中R表示烷基，环烷基，环烷基 - 烷基或芳烷基; 虚线表示存在或不存在键; 并且当存在键时，Z 2不存在，Z 1表示H，但当该键不存在时，Z 1表示 H和Z 2代表OH或Z 1和Z 2 2组合在一起，表示O或NOR 1或N 2 其中R 1表示H，或烷基，芳烷基或芳基; l代表0或1; A表示亚烷基，亚烯基或亚炔基; Y表示W表示CH，C-或N的基团; 当W为CH或N时，m代表0或1，当W为C-时，m代表1; B表示特定的二价基团; E 1和E 2各自独立地表示H或低级烷基; D表示芳族烃基或杂环基。 化合物（I）具有强的5-羟色胺-2受体拮抗作用，毒性低，副作用小，作为循环系统疾病如缺血性心脏病，脑血管障碍和周围循环障碍的治疗有用。

公开(公告)号：US20050228188A1

公开(公告)日：2005-10-13

申请号：US10511829

申请日：2003-04-28

申请人： Motoo Sumida ,  Masahiro Nakao ,  Namino Tomimori ,  Koshi Namikawa ,  Harukazu Fukami

发明人： Motoo Sumida ,  Masahiro Nakao ,  Namino Tomimori ,  Koshi Namikawa ,  Harukazu Fukami

IPC分类号： A23L1/29 ,  A23L1/30 ,  A61K8/37 ,  A61Q1/06 ,  C07C403/24 ,  C12P7/62 ,  C12P7/64 ,  C12P23/00 ,  C07C57/26

CPC分类号： C07C403/24 ,  A23L33/10 ,  A23L33/115 ,  A61K8/37 ,  A61Q1/06 ,  C12P7/62 ,  C12P7/6436 ,  C12P7/6454 ,  C12P23/00

摘要： The present invention provides an astaxanthin medium-chain fatty acid ester, which is expected to be applied in the fields of food, cosmetics and pharmaceuticals, and has higher digestibility and tissue penetration than long chain fatty acid ester form astaxanthins. When the synthesis of an astaxanthin medium-chain fatty acid ester is attempted using the catalytic action of lipase by conventional methods, ester cannot be formed. However, according to the present invention, a composition comprising an astaxanthin medium-chain fatty acid monoester and/or diester is produced by adding a certain amount of water into a reaction system and reacting an astaxanthin or a long chain fatty acid ester thereof with a medium-chain fatty acid, a triglyceride thereof or a suitable ester. Moreover, these monoesters and/or diesters are isolated, as necessary. The present invention further provides food or cosmetics comprising the composition of the present invention.

摘要翻译： 本发明提供了一种预期用于食品，化妆品和药物领域的虾青素中链脂肪酸酯，并且比长链脂肪酸酯形式的虾青素具有更高的消化率和组织穿透性。 当通过常规方法尝试使用脂肪酶的催化作用来合成虾青素中链脂肪酸酯时，不能形成酯。 然而，根据本发明，通过向反应体系中加入一定量的水并使虾青素或其长链脂肪酸酯与一种虾青素或其长链脂肪酸酯反应而制备包含虾青素中链脂肪酸单酯和/或二酯的组合物 中链脂肪酸，其甘油三酯或合适的酯。 此外，根据需要分离这些单酯和/或二酯。 本发明还提供包含本发明组合物的食品或化妆品。

公开(公告)号：US06921766B1

公开(公告)日：2005-07-26

申请号：US09869360

申请日：2000-11-01

申请人： Harukazu Fukami ,  Hidenori Urata

发明人： Harukazu Fukami ,  Hidenori Urata

IPC分类号： A61K31/517 ,  A61P9/10 ,  C07D239/96 ,  A61K31/513

CPC分类号： A61K31/517 ,  C07D239/96

摘要： There is provided a preventive or therapeutic agent for diseases accompanied by abnormal vascular function in which lipid deposition in the blood vessel is involved, said agent comprising a chymase inhibitor as an active ingredient.As the chymase inhibitor, a quinazoline derivative represented by the following formula is used. In the above formula, the ring A represents an aromatic ring.

摘要翻译： 提供了涉及血管功能异常的疾病的预防或治疗剂，其中涉及血管中的脂质沉积，所述药剂包含糜酶抑制剂作为活性成分。 作为胃促胰酶抑制剂，使用由下式表示的喹唑啉衍生物。 在上式中，环A表示芳香环。