公开(公告)号：US20170160281A1

公开(公告)日：2017-06-08

申请号：US15440136

申请日：2017-02-23

Applicant: Meso Scale Technologies, LLC.

Inventor： Anahit Aghvanyan ,  Eli N. Glezer ,  John Kenten ,  Sudeep Kumar ,  Galina Nikolenko ,  Martin Stengelin

IPC: G01N33/574

CPC classification number: G01N33/57423 ,  A61K39/39533

Abstract: The present invention relates to methods of diagnosing lung cancer in a patient, as well as methods of monitoring the progression of lung cancer and/or methods of monitoring a treatment protocol of a therapeutic agent or a therapeutic regimen. The invention also relates to assay methods used in connection with the diagnostic methods described herein.

公开(公告)号：US20160370359A1

公开(公告)日：2016-12-22

申请号：US15256364

申请日：2016-09-02

Applicant: MESO SCALE TECHNOLOGIES, LLC.

Inventor： George B. SIGAL ,  Jacob N. WOHLSTADTER ,  Satyanarayana GUDIBANDE ,  Mark T. MARTIN ,  James L. WILBUR

IPC: G01N33/543 ,  G01N33/553 ,  G01N33/552

CPC classification number: G01N33/54346 ,  B82Y30/00 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/532 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/5438 ,  G01N33/552 ,  G01N33/553 ,  G01N33/582 ,  G01N33/587 ,  G01N2458/30 ,  C12Q2563/149 ,  C12Q2563/113 ,  C12Q2537/125

Abstract: A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.

Abstract translation: 提出了一种用于进行电化学发光测定的方法和装置。 在本发明的示例性实施方案中，一种装置包括其上固定有第一测定配体的ECL非活性电极表面和其上固定有第二测定配体的ECL活性微粒。 在本发明的示例性实施方案中，方法包括（a）形成包含电化学发光标记和其上固定有第一测定配体的微粒的混合物，（b）在所述混合物存在下将电化学能施加到电极表面，以及 （c）测量发射的电化学发光，其中微粒是ECL-活性的，并且当在所述混合物的存在下施加电化学能时，电极表面是ECL-无活性的。

公开(公告)号：US20160349253A1

公开(公告)日：2016-12-01

申请号：US15206656

申请日：2016-07-11

Applicant: Meso Scale Technologies, LLC.

Inventor： Sudeep Kumar ,  George Sigal ,  Michael Tsionsky

IPC: G01N33/543 ,  C12Q1/68

CPC classification number: G01N33/54386 ,  C12Q1/6823 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/5306 ,  G01N2446/00 ,  G01N2458/30

Abstract: The invention relates to methods for conducting binding assays in an assay device that includes one or more storage and use zone. The storage zones of the assay device are configured to house one or more reagents used in an assay conducted in the use zone of the device.

公开(公告)号：US20140315742A1

公开(公告)日：2014-10-23

申请号：US14348275

申请日：2012-09-28

Applicant: MESO SCALE TECHNOLOGIES, LLC ,  THE HENRY M. JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE, INC.

Inventor： William F Blakely ,  Eli N. Glezer ,  John Kenten ,  Sudeep Kumar ,  Anu Mathew ,  Natalia I. Ossetrova ,  George Sigal

IPC: G01N33/566

CPC classification number: G01N33/56972 ,  G01N33/566 ,  G01N33/6893 ,  G01N2333/70535 ,  G01N2333/70578 ,  G01N2800/40 ,  G01N2800/60 ,  G01T1/00 ,  G01T1/02

Abstract: The present invention relates to methods and kits to assess an absorbed dose of ionizing radiation and/or the severity of tissue injury from radiation in a patient. The invention also relates to algorithms used to calculate an absorbed dose of radiation based on biomarker measurements of a plurality of biomarkers that are altered relative to a normal control in the event of radiation exposure.

Abstract translation: 本发明涉及用于评估电离辐射吸收剂量和/或患者辐射组织损伤严重程度的方法和试剂盒。 本发明还涉及用于基于在辐射暴露的情况下相对于正常对照改变的多个生物标志物的生物标志物测量来计算辐射的吸收剂量的算法。

公开(公告)号：US20140315189A1

公开(公告)日：2014-10-23

申请号：US14357653

申请日：2012-11-09

Applicant: Meso Scale Technologies, LLC

Inventor： Eli N. Glezer ,  George Sigal

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/70 ,  C12Q1/6813 ,  C12Q1/6834 ,  G01N33/542 ,  G01N33/54333 ,  C12Q2563/179

Abstract: The present invention is directed to methods for reducing cross-reactivity between species employed in multiplexed immunoassays.

Abstract translation: 本发明涉及降低多重免疫测定中使用的物种之间的交叉反应性的方法。

公开(公告)号：US20140274805A1

公开(公告)日：2014-09-18

申请号：US14250054

申请日：2014-04-10

Applicant: MESO SCALE TECHNOLOGIES LLC

Inventor： Jacob N. WOHLSTADTER ,  James L. WILBUR ,  George B. SIGAL ,  Mark T. MARTIN ,  Liang-Hong GUO ,  Alan FISCHER ,  Jon LELAND ,  Mark A. BILLADEAU ,  Larry R. HELMS ,  Ramin DARVARI

IPC: G01N21/76 ,  C12Q1/68

CPC classification number: G01N21/76 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0645 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6825 ,  G01N21/253 ,  G01N21/66 ,  G01N33/54313 ,  G01N33/5438 ,  G01N33/582 ,  G01N33/585 ,  Y10S435/808 ,  Y10S435/817 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807

Abstract: Materials and Methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.

Abstract translation: 提供材料和方法用于生产用于诊断的图案化多阵列多特异性表面。 本发明提供了用于检测或测量感兴趣分析物的电化学发光方法。 它还提供用于ECL测定的新型电极。 提供材料和方法用于化学和/或物理控制导电域和试剂沉积，以便使用多重特定的测试程序。

公开(公告)号：US20140221368A1

公开(公告)日：2014-08-07

申请号：US14168629

申请日：2014-01-30

Applicant: Meso Scale Technologies, LLC

Inventor： Simone Barbero ,  Eli N. Glezer ,  Anu Mathew

IPC: G01N33/574 ,  A61K31/5377

CPC classification number: G01N33/57415 ,  A61K31/5377 ,  C12Q1/42 ,  C12Q1/485 ,  G01N2333/912 ,  G01N2333/916 ,  G01N2800/52

Abstract: The present invention relates to methods of diagnosing breast cancer in a patient, as well as methods of monitoring the progression of breast cancer and/or methods of monitoring a treatment protocol of a therapeutic agent or a therapeutic regimen. The invention also relates to assay methods used in connection with the diagnostic methods described herein.

Abstract translation: 本发明涉及诊断患者乳腺癌的方法，以及监测乳腺癌进展的方法和/或监测治疗剂或治疗方案治疗方案的方法。 本发明还涉及结合本文所述的诊断方法使用的测定方法。

公开(公告)号：US20140191109A1

公开(公告)日：2014-07-10

申请号：US14147216

申请日：2014-01-03

Applicant: Meso Scale Technologies, LLC

Inventor： Ian Chamberlin ,  Charles M. Clinton ,  Eli N. Glezer ,  Bandele Jeffrey-Coker ,  Manish Kochar ,  Sandor Kovacs ,  D.T. Le ,  Aaron Leimkuehler ,  George Sigal ,  Leo Tabakin ,  Jon Willoughby

IPC: G02B7/36 ,  G01N27/26 ,  G01J1/20

CPC classification number: G02B7/36 ,  G01N21/253 ,  G01N21/6452 ,  G01N35/028

Abstract: Apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation are described. They are particularly well suited for conducting automated analysis in a multi-well plate assay format.

Abstract translation: 描述了用于进行测定的装置，系统，方法，试剂和试剂盒及其制备方法。 它们特别适用于以多孔板测定方式进行自动化分析。

公开(公告)号：US20030124572A1

公开(公告)日：2003-07-03

申请号：US10208526

申请日：2002-07-30

Applicant: favor of Meso Scale Technologies, LLC.

Inventor： Robert M. Umek ,  Paula Denney Eason ,  Gargi Maheshwari ,  Anu Mathew ,  Douglas Woods

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542

CPC classification number: G01N33/5438 ,  G01N21/253 ,  G01N21/6452 ,  G01N21/66 ,  G01N21/76

Abstract: Improved systems, system components, and methods for performing assays involving biological membranes and/or components thereof. Preferred luminescence test measurements are conducted using an assay module with integrated electrodes having biological membranes and/or components thereof immobilized thereon with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

Abstract translation: 用于进行涉及生物膜和/或其组分的测定的改进的系统，系统组件和方法。 使用具有固定有生物膜和/或其组分的集成电极的测定模块进行优选的发光测试测量，其具有适于接收测定模块的读取器装置，在测定的孔或测定区域中诱导发光，优选电极诱导的发光 模块并测量诱导的发光。

公开(公告)号：US20030113713A1

公开(公告)日：2003-06-19

申请号：US10238391

申请日：2002-09-10

Applicant: Meso Scale Technologies, LLC

Inventor： Eli N. Glezer ,  Kent Johnson ,  Michael Tsionsky ,  John H. Kenten ,  Jeff D. DeBad ,  Robert M. Umek ,  Paula Denney Eason ,  Hans Biebuyck ,  Jacob N. Wohlstadter ,  James Wilbur ,  George Sigal

IPC: C12Q001/70 ,  C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N033/537 ,  G01N033/543 ,  C12M001/34

CPC classification number: G01N33/5304 ,  C12Q1/485 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/54386 ,  G01N33/56944 ,  G01N33/56983 ,  G01N33/56988 ,  G01N33/573 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6845 ,  G01N2333/11 ,  G01N2333/135 ,  G01N2333/16 ,  G01N2333/525 ,  G01N2333/5412 ,  G01N2333/545 ,  G01N2333/57 ,  G01N2333/71 ,  G01N2333/9121 ,  Y10S436/807

Abstract: Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

Abstract translation: 使用具有多个测定域的测定模块进行多重测试测量。 在优选实施方案中，这些测量在具有集成电极的测定模块中进行，所述测量模块具有适于接收测定模块的读取器装置，在测定模块的孔或测定区域中诱导发光，优选电极诱导的发光并测量诱导的发光。