公开(公告)号：US09404151B2

公开(公告)日：2016-08-02

申请号：US13695254

申请日：2011-04-28

申请人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/68 ,  B01L7/00 ,  C12Q3/00

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/1816 ,  C12Q3/00

摘要： The present disclosure provides a non-contact real time micro Polymerase Chain Reaction (PCR) system comprising; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising an associated LED driver and a photo detector amplifier placed above the chip to detect fluorescence; an induction heater mounted around the chip and inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein the infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.

摘要翻译： 本公开提供了非接触式实时微聚合酶链式反应（PCR）系统，其包含： 具有用于保持样品的反应室和用于加热样品的反应室下面的嵌入式金属加热器的芯片; 光学单元，包括相关联的LED驱动器和放置在芯片上方以检测荧光的光电检测放大器; 安装在芯片周围并感应耦合到金属加热器的感应加热器; 安装在所述芯片下方的用于测量所述金属加热器的温度的红外温度传感器，其中所述红外温度传感器与信号调节器接口; 以及与信号调节器和感应加热器接口的控制器，其基于通过信号调节器从红外温度传感器接收的反馈来调节对感应加热器的电力。

公开(公告)号：US20130203150A1

公开(公告)日：2013-08-08

申请号：US13520685

申请日：2011-01-06

申请人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07H1/08

CPC分类号： C07H1/08 ,  C12N15/10 ,  C12N15/1006

摘要： The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.

摘要翻译： 本公开提供了使用棉花从固体或液体样品分离天然和人造核酸如脱氧核糖核酸（DNA），核糖核酸（RNA）和肽核酸（PNA）的方法。 包装的棉花使得含有核酸的溶液通过它，并且溶液中的核酸在最适于结合的培养基中与棉花结合。 核酸以这样的方式结合棉花，使得结合的核酸可以经受含有水的液体的多次洗涤，并在水性缓冲液中洗脱，其中洗脱的核酸可以直接用于使用PCR或任何其它的扩增 生物化学或分子生物学的需要。

公开(公告)号：US20130101983A1

公开(公告)日：2013-04-25

申请号：US13695254

申请日：2011-04-28

申请人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/68 ,  C12Q3/00

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/1816 ,  C12Q3/00

摘要： The present disclosure provides a non contact real time micro Polymerase Chain Reaction [PCR] system comprises; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising associated LED driver and photo detector amplifier placed above the chip to detect the fluorescence; an induction heater mounted around the chip and is inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein said infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.

摘要翻译： 本公开提供了非接触式实时微聚合酶链式反应[PCR]系统，包括： 具有用于保持样品的反应室和用于加热样品的反应室下面的嵌入式金属加热器的芯片; 光学单元，包括放置在芯片上方的相关联的LED驱动器和光电检测放大器，以检测荧光; 感应加热器，安装在芯片周围，并感应耦合到金属加热器; 安装在所述芯片下方的用于测量所述金属加热器的温度的红外温度传感器，其中所述红外温度传感器与信号调节器接口; 以及控制器，其与信号调节器和感应加热器接口，用于基于通过信号调节器从红外温度传感器接收的反馈来调节对感应加热器的电力。

公开(公告)号：US08282692B2

公开(公告)日：2012-10-09

申请号：US12452397

申请日：2008-06-26

申请人： Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Phanikumar Pullela ,  Gopalkrishna Mangalore Kini

发明人： Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Phanikumar Pullela ,  Gopalkrishna Mangalore Kini

IPC分类号： C10L1/00 ,  C10L1/18

CPC分类号： C10L1/026 ,  C10G2300/1014 ,  C10G2300/1051 ,  C10G2300/1055 ,  C10G2300/80 ,  Y02E50/13 ,  Y02P30/20

摘要： The present invention relates to a biofuel composition comprising distilled technical cashew nut shell liquid (DT-CNSL) and atleast one petroleum product optionally alongwith plant oils and fuel additive(s). It also relates to a process for preparation of biofuel composition and a method of fueling biofuel composition.

摘要翻译： 本发明涉及一种生物燃料组合物，其包含蒸馏的技术腰果壳液（DT-CNSL）和至少一种石油产品，任选地与植物油和燃料添加剂一起。 它还涉及生物燃料组合物的制备方法和燃料生物燃料组合物的方法。

公开(公告)号：US20120045761A1

公开(公告)日：2012-02-23

申请号：US13203286

申请日：2010-02-23

申请人： Manjula Jagannath ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Manjula Jagannath ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/68

CPC分类号： C12Q1/701 ,  C12Q2561/101 ,  C12Q2561/113 ,  Y02A50/51

摘要： The present disclosure gives a detailed description of methods for determining the presence of Chikungunya viral nucleic acids in blood/serum/plasma samples by employing “Oligonucleotide” probes. The designed “Oligonucleotide” probes can be used for qualitative or quantitative detection of Chikungunya virus in an infected sample by employing Real time PCR.

摘要翻译： 本公开详细描述了通过使用“寡核苷酸”探针来测定血液/血清/血浆样品中基孔肯雅病毒核酸的存在的方法。 设计的“寡核苷酸”探针可用于通过采用实时PCR定性或定量检测感染样品中基孔肯雅病毒。

公开(公告)号：US20110207114A1

公开(公告)日：2011-08-25

申请号：US13126204

申请日：2009-10-29

申请人： Anil Kumar ,  Jasmine Sinha ,  Phani Kumar Pullela

发明人： Anil Kumar ,  Jasmine Sinha ,  Phani Kumar Pullela

IPC分类号： C12Q1/00 ,  G01N33/00 ,  G01N33/50 ,  G01N33/92 ,  C08G75/12 ,  C08F228/06 ,  C08F238/00

CPC分类号： C08G61/02 ,  C08G2261/148 ,  C08G2261/312 ,  C08G2261/3422 ,  C09K11/06 ,  C09K2211/1416 ,  G01N2021/7786 ,  Y10T436/143333 ,  Y10T436/163333 ,  Y10T436/177692

摘要： The present disclosure is related to amplified fluorescence polymers (AFPs) with pendant functional groups, their derivatives and their synthesis. The amplified fluorescence polymers can be used in various biological and chemical sensors.

摘要翻译： 本公开涉及具有侧链官能团，其衍生物及其合成的扩增荧光聚合物（AFP）。 扩增的荧光聚合物可用于各种生物和化学传感器。

公开(公告)号：US20110197498A1

公开(公告)日：2011-08-18

申请号：US13122962

申请日：2009-08-10

申请人： Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Phani Kumar Pullela ,  Gopalakrishna Mangalore Kini

发明人： Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Phani Kumar Pullela ,  Gopalakrishna Mangalore Kini

IPC分类号： C10L1/182

CPC分类号： C10L1/026 ,  C10L1/1802 ,  C10L1/1824 ,  C10L1/19 ,  Y02E50/13

摘要： The present invention relates to a biofuel composition comprising normal chain alcohol(s), natural oils and diesel optionally along with fuel additive(s); a process of preparation and a method of fueling said biofuel composition.

摘要翻译： 本发明涉及包含常规链醇，天然油和柴油的生物燃料组合物，任选地与燃料添加剂一起; 制备方法和加油所述生物燃料组合物的方法。

公开(公告)号：US10525461B2

公开(公告)日：2020-01-07

申请号：US15251671

申请日：2016-08-30

申请人： BIGTEC PRIVATE LIMITED

发明人： Manoj Mulakkapurath Narayanan ,  Chandrasekhar Bhaskaran Nair

IPC分类号： B01L3/00

摘要： The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.

公开(公告)号：US09228144B2

公开(公告)日：2016-01-05

申请号：US13122962

申请日：2009-08-10

申请人： Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Phani Kumar Pullela ,  Gopalakrishna Mangalore Kini

发明人： Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao ,  Phani Kumar Pullela ,  Gopalakrishna Mangalore Kini

IPC分类号： C10L1/182 ,  C10L1/02 ,  C10L1/18 ,  C10L1/19

CPC分类号： C10L1/026 ,  C10L1/1802 ,  C10L1/1824 ,  C10L1/19 ,  Y02E50/13

摘要： The present invention relates to a biofuel composition comprising normal chain alcohol(s), natural oils and diesel optionally along with fuel additive(s); a process of preparation and a method of fueling said biofuel composition.

摘要翻译： 本发明涉及包含常规链醇，天然油和柴油的生物燃料组合物，任选地与燃料添加剂一起; 制备方法和加油所述生物燃料组合物的方法。

公开(公告)号：US20140057318A1

公开(公告)日：2014-02-27

申请号：US13973130

申请日：2013-08-22

申请人： Bigtec Private Limited

发明人： Mulakkapurath Narayanan Manoj ,  Venkata Ramachandra Rao Vasamsetty ,  Madhuri Baliga ,  Kirubakaran Naveen Kumar ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07K14/62

CPC分类号： C07K1/18 ,  C07K14/62 ,  C12N2800/22

摘要： The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.