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公开(公告)号：US20220258130A1
公开(公告)日：2022-08-18
申请号：US17735882
申请日：2022-05-03
Applicant: Cytiva BioProcess R&D AB
Inventor： Ann Catharina Bergh , Mattias Algotsson , Tania Ahmad , Therese Graner
IPC: B01J20/289 , B01D15/38 , B01J20/28 , B01J20/32
Abstract: The present invention relates to a novel chromatography media, more closely a novel IMAC (Immobilized Metal Affinity Chromatography) media. The novel chromatography media comprises a pentaligand and provides high dynamic binding capacity as well as high purity of the sample proteins purified on the media of the invention.
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公开(公告)号：US11352388B2
公开(公告)日：2022-06-07
申请号：US15564224
申请日：2016-04-04
Applicant: Cytiva BioProcess R&D AB
Inventor： Martin Hall , Mikael Berg , Ida Eklind , Sandeep Kristiansson
Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of Factor VIII and von Willebrand factor from a cryoprecipitate of plasma. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.
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公开(公告)号：US11141681B2
公开(公告)日：2021-10-12
申请号：US15458667
申请日：2017-03-14
Applicant: Cytiva BioProcess R&D AB
Inventor： Klaus Gebauer
Abstract: The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.
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公开(公告)号：US20210292788A1
公开(公告)日：2021-09-23
申请号：US17047843
申请日：2019-04-24
Applicant: Cytiva BioProcess R&D AB
Inventor： Asa Hagner McWhirter , Anna Akerblom
Abstract: The present invention relates to a method for virus purification. The present invention provides downstream processes for purification of adenovirus from cell culture harvest. More closely, it relates to a method for adenovirus purification using a virus capture and a virus polishing step.
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公开(公告)号：US11124539B2
公开(公告)日：2021-09-21
申请号：US16348534
申请日：2017-11-14
Applicant: Cytiva BioProcess R&D AB
Inventor： Johan William Ohman , Mats Ander , Therese Graner , Anneli Jorsback , Jinyu Zou
IPC: C07K1/22 , B01J20/32 , B01D15/38 , B01J20/24 , B01J20/285 , B01J20/286
Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.
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Patent Agency Ranking
公开(公告)号：US20210163529A1
公开(公告)日：2021-06-03
申请号：US16955119
申请日：2018-12-13
Applicant: Cytiva Bioprocess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Ronnie Palmgren , Tomas Bjorkman
Abstract: A method for preparation of a separation matrix, comprising the steps of: a) providing a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein; b) reacting said alkali-stable ligand with said solid support to form a separation matrix having covalently coupled alkali-stable ligands; and c) washing said separation matrix having covalently coupled alkali-stable ligands with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
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公开(公告)号：US20250066757A1
公开(公告)日：2025-02-27
申请号：US18719120
申请日：2023-02-20
Applicant: Cytiva BioProcess R&D AB
Inventor： Simon Aberg , Linda Hagman , Mikael Berg , Hans Blom , Albin Larsson , Joakim Bagge , Elin Monie , Markus Pitkanen
IPC: C12N15/10
Abstract: The present disclosure is directed to a method for separating supercoiled plasmid DNA (pDNA) from a liquid sample. the method comprising the steps of: (a) adding a liquid sample comprising pDNA to a first chromatography material comprising (i) an anion exchange chromatography ligand for binding to pDNA and (ii) a support material allowing convective flow through the first chromatography material, wherein the liquid sample originates from a cell culture harvest and has been subjected to a step of removing RNA before step (a): (b) eluting a liquid sample. comprising a purified mixture of supercoiled pDNA and open circular pDNA, from the first chromatography material: (c) adding the liquid sample from step (b) to a second chromatography material comprising a ligand that binds to pDNA and enables selective separation of supercoiled pDNA from open circular pDNA: (d) eluting the purified supercoiled pDNA from the second chromatography material: wherein the supercoiled pDNA eluted in step (d) has a purity degree of at least 95% without use of any further chromatography material than said first and second chromatography materials. Steps (a)-(d) and any intermediate steps can be completed within 5 hours. Further disclosed are uses of supercoiled pDNA obtained by said separation method.
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公开(公告)号：US20250034593A1
公开(公告)日：2025-01-30
申请号：US18913493
申请日：2024-10-11
Applicant: Cytiva BioProcess R&D AB
Inventor： Asa Hagner McWhirter , Anna Akerblom
IPC: C12N15/86 , C12N7/00 , C12N15/861
Abstract: The present invention relates to a method for virus purification. The present invention provides downstream processes for purification of adenovirus from cell culture harvest. More closely, it relates to a method for adenovirus purification using a virus capture and a virus polishing step.
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公开(公告)号：US12146149B2
公开(公告)日：2024-11-19
申请号：US17047843
申请日：2019-04-24
Applicant: Cytiva BioProcess R&D AB
Inventor： Asa Hagner McWhirter , Anna Akerblom
IPC: C12N15/86 , C12N7/00 , C12N15/861
Abstract: The present invention relates to a method for virus purification. The present invention provides downstream processes for purification of adenovirus from cell culture harvest. More closely, it relates to a method for adenovirus purification using a virus capture and a virus polishing step.
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公开(公告)号：US12037359B2
公开(公告)日：2024-07-16
申请号：US17114773
申请日：2020-12-08
Applicant: CYTIVA BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander
IPC: C07K1/22 , B01D15/38 , B01J20/24 , B01J20/26 , B01J20/28 , B01J20/285 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/06 , C07K16/12 , C07K17/10
CPC classification number: C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/267 , B01J20/28016 , B01J20/28078 , B01J20/285 , B01J20/286 , B01J20/3212 , B01J20/3274 , C07K14/31 , C07K16/00 , C07K16/065 , C07K16/1271 , C07K17/10 , B01J2220/52
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 22, SEQ ID NO: 51 or SEQ ID NO: 52, wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO: 4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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